Metabolic Stress Indicators in Ewes (Ovis aries) under Post-parturient and High Protein Diet Conditions

Farman, M; Tripathi, Shiv; Tej, Nikhil Kumar; Nandi, S.; Gupta, P. S. P.; Mondal, Sukanta; Venkatesh, G

doi:10.3923/ajava.2018.360.368

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…During the post parturient condition or in a high protein diet feeding ewes were mainly encountered with elevated concentration of metabolic load (Farman et al, 2018). Elevated metabolic stressors led to reduced viability, oocyte maturation and growth rate.…”

Section: Discussionmentioning

confidence: 99%

“…To test whether the reprogramming event was susceptible to the elevated concentration of metabolic stressors, ovine oocytes were exposed to elevated concentration of metabolic stressors (ammonia, NEFA, BHB and urea) which were found to cause a significant decline in oocytes development in earlier studies in our laboratory (Nandi et al, 2017(Nandi et al, , 2018. These metabolic stressors (ammonia, NEFA, BHB and urea) were commonly present in follicular fluid and serum of ewes during the post-parturient and the high protein diet feeding conditions (Farman et al, 2018). We then quantified global DNA methylation and the expression of DNA methyltransferase transcript (DNMT1, DNMT3A and DNMT3B) in matured oocytes and embryos produced.…”

Section: Introductionmentioning

confidence: 99%

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Global DNA methylation, DNA methyltransferase and stress‐related gene expression in ovine oocytes and embryos after exposure to metabolic stressors

Nandi

Tripathi

Singh

et al. 2023

Reprod Domestic Animals

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DNA methylation, considered the most prominent epigenetic mark was important for the gene regulation in embryonic development. The present study aimed at evaluating the effects of metabolic stressors [Non‐esterified fatty acid (NEFA), β‐hydroxy‐butyric acid (BHB), ammonia and urea] exposure during the in vitro ovine oocyte maturation, global DNA methylation, DNA methyltransferase and stress‐related gene expression. Colorimetric analysis of global DNA methylation and the expression of the DNA methyltransferase genes (DNMT1, DNMT3A, and DNMT3B) were assessed in the matured oocytes, 2‐cell embryos and blastocysts produced in vitro from oocytes exposed with the metabolic stressors during 24 h of the in vitro maturation (IVM). Further, the mRNA expression of the stress‐related genes (SOD1, SOD2) in the matured oocytes, 2‐cell embryos and blastocysts produced was assessed. Significant difference in global DNA methylation levels between all the treatments tested was observed when compared with control in oocytes, two‐cell embryos and blastocysts. Elevated concentration of metabolic stressors resulted in increased expressions of several stress‐related genes, i.e., SOD1, SOD2 and in mRNA expression of DNA methyltransferase genes. The present study is the first to report that the DNA methylation was sensitive to the effects of the metabolic stressors in ovine oocytes/embryos. The aberrant expressions of genes during oocyte development targeted in the present study can provide evidence for the early embryo developmental arrest and blastocysts quality. These results highlighted the sensitivity of the early embryogenesis and more precisely of the reprogramming period to metabolites challenges, in a realistic situation of elevated concentration of metabolic stressors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Global DNA methylation, DNA methyltransferase and stress‐related gene expression in ovine oocytes and embryos after exposure to metabolic stressors

Nandi

Tripathi

Singh

et al. 2023

Reprod Domestic Animals

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“…After treatment, the serum concentration of Free T4 was 25.17 pmol/L. Few studies have recorded cases of goiter in sheep in which they have quantified thyroid hormones in their free form, as it is common to verify records with Total T4 and T3 (Kallfez and Erali, 1973;Reap et al, 1978;Bowers et al, 1993;Starling et al, 2005;Todini, 2007;Iveta et al, 2011;Hassan et al, 2013 andFarman et al, 2018); whereas Anderson et al (1988) and Oznem et al (2005), quantified Total and Free T4 and T3. Alterations in serum concentrations of Free T4 and Free T3 generally followed those of Total T4 and T3, Research, Society and Development, v. 9, n. 9, e203996887, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i9.6887 10 respectively, as observed by Fazio et al (2007), since this assumption was confirmed by the existence of positive and significant correlations between the concentrations of Total T3 with Free T3 and Total T4 with Free T4.…”

Section: Quantification Of Free T3 and T4mentioning

confidence: 99%

Hyperplastic goiter in sheep in the semi-arid region of Paraiba, Brazil: case report

Tolentino

Rocha

Filho

et al. 2020

RSD

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The aim of this study was to describe the clinical and anatomopathological findings and serum concentrations of free triiodothyronine (T3) and thyroxine (T4) from an outbreak of iodine (I) deficiency in sheep in the semi-arid region of Brazil. The outbreak was observed in a property located in the municipality of Sousa, West of the State of Paraiba, a semi-arid zone of Northeast Brazil. The studied lot was composed of 14 animals, between 12 and 18 months old, male, and mixed breed. The animals presented an increase in volume in the proximal portion of the neck, ventral to the mandibular arch, of firm consistency, suggesting a goiter. Thyroid samples were collected from animals that presented gland hyperplasia. In addition, blood samples were collected for serum analysis of thyroid hormones. The clinical findings, macroscopic characteristics, and histological findings observed in these cases are identical to those described in the literature for acquired goiter and constitute evidence that allows the definitive diagnosis of this condition. The mean concentrations observed in animals were: Free T3 (0.5±0.08 pmol/L) and Free T4 (10.93±2.88 pmol/L). Based on the epidemiological findings, clinical signs, anatomopathological lesions, and serum analysis of thyroid hormones, we can affirm the outbreak of acquired goiter occurred due to inadequacies in feeding and mineral supplementation of the animals. Thus, in order to prevent the appearance of this nutritional disorder, it is of utmost importance that animals are provided with a balanced mineral diet and supplementation, taking into account the needs of the species and antithyroid feed substances.

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Molecular Insights of Compromised Female Reproduction in Ruminants Under Metabolic and Nutritional Stress

Nandi

Tripathi

Gupta

et al. 2022

Sustainable Agriculture Reviews

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Metabolic Stress Indicators in Ewes (Ovis aries) under Post-parturient and High Protein Diet Conditions

Cited by 3 publications

References 19 publications

Global DNA methylation, DNA methyltransferase and stress‐related gene expression in ovine oocytes and embryos after exposure to metabolic stressors

Global DNA methylation, DNA methyltransferase and stress‐related gene expression in ovine oocytes and embryos after exposure to metabolic stressors

Hyperplastic goiter in sheep in the semi-arid region of Paraiba, Brazil: case report

Molecular Insights of Compromised Female Reproduction in Ruminants Under Metabolic and Nutritional Stress

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