Metabolic variability within individual fibres of the cat tibialis posterior and diaphragm muscles

Blanco, Cesar E.; Fournier, Mario; Sieck, Gary C.

doi:10.1007/bf01042182

Cited by 21 publications

(12 citation statements)

References 34 publications

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“…The between-day mean differences for measurements increased by ,-,0.1% for a sample of ,-~50 fibres and by ,--0.5% for a single fibre. The between-trials difference in SDH activity determined within-day and between-day is likely to be due, in part, to the possibility that SDH activity may vary along the length of muscle fibres (Nemeth et al, 1981;Blanco et al, 1988Blanco et al, , 1991. The estimated distance between the groups of sections for the within-and between-day measurements was 70-100 ~m and 200-250 ~m, respectively.…”

Section: Reliability Of the Histochemical Sdh Measurementmentioning

confidence: 91%

Biochemical verification of quantitative histochemical analysis of succinate dehydrogenase activity in skeletal muscle fibres

et al. 1993

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The purpose of this investigation was to examine critically the validity of a computerized quantitative microphotometric histochemical technique for the determination of succinate dehydrogenase (SDH) activity in skeletal muscle fibres. Sections from the anterior costal diaphragm were removed from Fischer-344 rats (n = 12) and assayed histochemically to determine SDH activity. The SDH activity in individual muscle fibres was computed using a computerized microphotometric histochemical technique which involves measurement of the optical density of deposited diformazan derived from nitroblue tetrazolium within the fibres. To validate the histochemical technique, whole muscle SDH activities were calculated from the histochemical procedure and were compared to SDH activities determined from whole muscle homogenates via a standard quantitative biochemical assay. The mean within-day variability of the computerized microphotometric histochemical technique of determining SDH activity was 6% (range = 0.5-10.9%) for an area containing approximately 50 fibres and 6.1% (range = 1.05-14.9%) for an individual muscle fibre. Similarly, the mean between-day variability of the microphotometric histochemical technique of determining SDH activity was 5.9% (range = 2.6-13.9%) for an area containing approximately 50 fibres and 6.6% (range = 2.2-13.9%) for an individual muscle fibre. The inter-class correlation coefficient between biochemically determined SDH activity and histochemically determined SDH activity was r = 0.83 (p < 0.05). Collectively, these data demonstrate that the quantitative histochemical technique of Blanco et al. (1988) is both valid and reliable in the determination of SDH activity in skeletal muscle fibres.

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Section: Reliability Of the Histochemical Sdh Measurementmentioning

confidence: 91%

Biochemical verification of quantitative histochemical analysis of succinate dehydrogenase activity in skeletal muscle fibres

et al. 1993

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“…In this regard, methods were developed to quantify both actomyosin ATPase and succinate dehydrogenase activities (35, 37, 275). Using these quantitative techniques, a number of studies have shown that the metabolic properties of diaphragm muscle fibers are continuous and highly plastic under a variety of conditions (36, 204, 237, 377, 381, 389, 390, 466). …”

Section: Respiratory Muscle Structurementioning

confidence: 99%

Mechanical Properties of Respiratory Muscles

Sieck

Ferreira

Reid

et al. 2013

Comprehensive Physiology

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Striated respiratory muscles are necessary for lung ventilation and to maintain the patency of the upper airway. The basic structural and functional properties of respiratory muscles are similar to those of other striated muscles (both skeletal and cardiac). The sarcomere is the fundamental organizational unit of striated muscles and sarcomeric proteins underlie the passive and active mechanical properties of muscle fibers. In this respect, the functional categorization of different fiber types provides a conceptual framework to understand the physiological properties of respiratory muscles. Within the sarcomere, the interaction between the thick and thin filaments at the level of cross-bridges provides the elementary unit of force generation and contraction. Key to an understanding of the unique functional differences across muscle fiber types are differences in cross-bridge recruitment and cycling that relate to the expression of different myosin heavy chain isoforms in the thick filament. The active mechanical properties of muscle fibers are characterized by the relationship between myoplasmic Ca2+ and cross-bridge recruitment, force generation and sarcomere length (also cross-bridge recruitment), external load and shortening velocity (cross-bridge cycling rate), and cross-bridge cycling rate and ATP consumption. Passive mechanical properties are also important reflecting viscoelastic elements within sarcomeres as well as the extracellular matrix. Conditions that affect respiratory muscle performance may have a range of underlying pathophysiological causes, but their manifestations will depend on their impact on these basic elemental structures.

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“…Five muscle samples each of YC and YT group and four muscle samples each of MC and MT group were used. The staining protocol used has been described previously (Martin et al 1985, Blanco et al 1991. Sections 10-µm-thick (four sections per muscle) were incubated in a medium containing 100 m phosphate buffer, pH 7.6 ; 0.75 m sodium azide ; 1 m 1methoxyphenazine methylsulfate (mPMS) ; 1.5 m nitroblue tetrazolium (NBT) ; 5 m EDTA (disodium salt) ; and 48 m succinate (disodium salt).…”

Section: Histochemical Procedures For Succinate Dehydrogenase (Sdh) Amentioning

confidence: 99%

Effects of treadmill training on the arteriolar and venular portions of capillary in soleus muscle of young and middle‐aged rats

Suzuki

Gao

Batra

et al. 1997

Acta Physiologica Scandinavica

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The effects of a 6-week programme of endurance training on soleus muscle capillarity were examined, in terms particularly of the proportions of arteriolar and venular capillaries and their capillary domain area, in young (3-week-old) and middle-aged (54-week-old) Wistar rats. Exercise protocols for the young training group were: 10-22.5 m min-1 60 min day-1 for 6 days a week, with a gradient of 7 degrees during the final 2 weeks; for the middle-aged training group, the protocols were: 10-20 m min-1. 50 min day-1 for 6 days a week. In both young and middle-aged training groups, the density of arteriolar capillaries was significantly increased (P < 0.05), but that of venular and intermediate capillaries was decreased slightly. The proportion of arteriolar capillaries therefore was significantly (P < 0.05) increased, from 63.9 to 73.1% in young rats and from 33.0 to 48.4%, in middle-aged rats after training. The increase in the proportion of arteriolar capillaries is an advantageous adaptation to exercise-induced increases in oxygen demand. In both young and middle-aged rats, capillary domain area and Krogh's tissue cylinder radii in all capillary portions decreased after training. These results suggest that adaptive changes in oxygen transport system, identified as an increase in the arteriolar capillary and a reduction in diffusion distance for oxygen, were observed in middle-aged as well as in young rats. However, capillary angiogenesis induced by exercise appeared to be greater in young than in middle-aged rats.

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Metabolic variability within individual fibres of the cat tibialis posterior and diaphragm muscles

Cited by 21 publications

References 34 publications

Biochemical verification of quantitative histochemical analysis of succinate dehydrogenase activity in skeletal muscle fibres

Biochemical verification of quantitative histochemical analysis of succinate dehydrogenase activity in skeletal muscle fibres

Mechanical Properties of Respiratory Muscles

Effects of treadmill training on the arteriolar and venular portions of capillary in soleus muscle of young and middle‐aged rats

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