Metabolically engineered methylotrophic yeast cells and enzymes as sensor biorecognition elements

Гончар, М. В.; Maidan, Mykola; Korpan, Yaroslav I.; Sibirny, Vladimir; Kotylak, Zbigniew; Sibirny, Andriy А.

doi:10.1111/j.1567-1364.2002.tb00099.x

Cited by 13 publications

(14 citation statements)

References 9 publications

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“…The drawback of this method is the requirement for expensive apparatus as well as the necessity to remove oxygen traces by sparging samples with pure nitrogen. Amperometric sensors (Winter and Cammann, 1989;Hall et al, 1998;Vastarella and Nicastri, 2005), potentiometric detection schemes (Vianello et al, 1996;Korpan et al, 1993Korpan et al, , 1997Korpan et al, , 2000Gonchar et al, 2002) and optical sensors (Rindt and Scholtissek, 1989;Kawamura et al, 2005) biosensors have been suggested for the determination of formaldehyde concentration.…”

Section: Introductionmentioning

confidence: 98%

Formaldehyde-sensitive sensor based on recombinant formaldehyde dehydrogenase using capacitance versus voltage measurements

Ali

Гончар

Gayda

et al. 2007

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 98%

Formaldehyde-sensitive sensor based on recombinant formaldehyde dehydrogenase using capacitance versus voltage measurements

Ali

Гончар

Gayda

et al. 2007

Biosensors and Bioelectronics

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“…The drawback of this method is that it requires an expensive apparatus and the removing of oxygen traces by purging samples with pure nitrogen. Amperometric (Winter and Cammann, 1989;Hall et al, 1998;Vastarella and Nicastri, 2005), potentiometric (Vianello et al, 1996;Korpan et al, 1993Korpan et al, , 1997Korpan et al, , 2000Gonchar et al, 2002), and optical (Rindt and Scholtissek, 1989;Kawamura et al, 2005) biosensors have been suggested for the determination of formaldehyde concentration. In spite of these developments, some serious problems remain unsolved, for example: (1) the necessity to regenerate NAD (for formaldehyde dehydrogenase (FDH)-based sensors; (2) low sensitivity (for potentiometric ones); (3) low stability; and (4) rather short linear range.…”

Section: Introductionmentioning

confidence: 98%

Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane

Ali¹,

Korpan

Гончар

et al. 2006

Biosensors and Bioelectronics

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“…For example, oxidative stress responses and cellular reorganization associated with vanadate tolerance in H. polymorpha have been investigated (31,32). Metabolically engineered H. polymorpha strains were also developed as the sensing bioelements in a whole-cell biosensor to detect alcohols and formaldehyde (12,26).…”

mentioning

confidence: 99%

Identification of the Cadmium-Inducible Hansenula polymorpha SEO1 Gene Promoter by Transcriptome Analysis and Its Application to Whole-Cell Heavy-Metal Detection Systems

Park

Sohn

et al. 2007

Appl Environ Microbiol

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The genomewide gene expression profiling of the methylotrophic yeast Hansenula polymorpha exposed to cadmium (Cd) allowed us to identify novel genes responsive to Cd treatment. To select genes whose promoters can be useful for construction of a cellular Cd biosensor, we further analyzed a set of H. polymorpha genes that exhibited >6-fold induction upon treatment with 300 M Cd for 2 h. The putative promoters, about 1,000-bp upstream fragments, of these genes were fused with the yeast-enhanced green fluorescence protein (GFP) gene. The resultant reporter cassettes were introduced into H. polymorpha to evaluate promoter strength and specificity. The promoter derived from the H. polymorpha SEO1 gene (HpSEO1) was shown to drive most strongly the expression of GFP upon Cd treatment among the tested promoters. The Cd-inducible activity was retained in the 500-bp deletion fragment of the HpSEO1 promoter but was abolished in the further truncated 250-bp fragment. The 500-bp HpSEO1 promoter directed specific expression of GFP upon exposure to Cd in a dose-dependent manner, with Cd detection ranging from 1 to 900 M. Comparative analysis of the Saccharomyces cerevisiae SEO1 (ScSEO1) promoter revealed that the ScSEO1 promoter has a broader specificity for heavy metals and is responsive to arsenic and mercury in addition to Cd. Our data demonstrate the potential use of the HpSEO1 promoter as a bioelement in whole-cell biosensors to monitor heavy metal contamination, particularly Cd.Industrial activities lead to large-scale environmental contamination with toxic heavy metals, such as cadmium (Cd) and mercury (Hg). These metals are toxic even at low levels and tend to accumulate in the body over an extended period of time, which can eventually cause serious health problems in humans. Therefore, the development of monitoring systems for these metal ions in the environment has become increasingly important in order to prevent chronic exposure to these pollutants (2). As a response to this need, numerous biological systems and nonbiological sensors based on the emerging nanotechnology have been developed to monitor heavy metal contamination (38, 43). In particular, biosensors that use unicellular microorganisms as analytical tools to monitor heavy metals have drawn attention because of several practical advantages (1): the large population size, rapid growth rate, low cost, and easy maintenance. Moreover, the feasibility of genetic manipulation makes microbial cells an attractive choice as environmental bioreporters.Many current microbial whole-cell sensors are based on genetically modified microorganisms (43,28). In general, microbial biosensors comprise the molecular fusion of two linked genetic elements: a sensing bioelement and a reporter gene. In most cases, the sensing element is a promoter that specifically responds to the presence or absence of the target molecule, and the reporter gene, which is fused to the sensing element, encodes a quantifiable molecule such as a bioluminescent or fluorescent protein (13). In creating ...

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Metabolically engineered methylotrophic yeast cells and enzymes as sensor biorecognition elements

Cited by 13 publications

References 9 publications

Formaldehyde-sensitive sensor based on recombinant formaldehyde dehydrogenase using capacitance versus voltage measurements

Formaldehyde-sensitive sensor based on recombinant formaldehyde dehydrogenase using capacitance versus voltage measurements

Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane

Identification of the Cadmium-Inducible Hansenula polymorpha SEO1 Gene Promoter by Transcriptome Analysis and Its Application to Whole-Cell Heavy-Metal Detection Systems

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