Metabolically Engineered Yeasts: ‘Potential’ Industrial Applications

Branduardi, Paola; Smeraldi, Carla; Porro, Danilo

doi:10.1159/000111990

Cited by 27 publications

(21 citation statements)

References 94 publications

(43 reference statements)

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“…Saccharomyces cerevisiae is a unicellular yeast and one of the most explored organism in terms of industrial applications and genetic studies [8]. Several previous studies showed that members of Saccharomyces genus can possess anti-bacterial and probiotic properties [9].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Fakruddin

Hossain

Ahmed

2017

BMC Complement Altern Med

147

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BackgroundProbiotic yeast has become a field of interest to scientists in recent years.MethodsConventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties.ResultsThis study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme. It can produce organic acid and showed resistance against tetracycline, ampicillin, gentamycin, penicillin, polymixin B and nalidixic acid. It can assimilate cholesterol, can produce killer toxin, vitamin B12, glutathione, siderophore and strong biofilm. It showed moderate auto-aggregation ability and cell surface hydrophobicity. The isolate can produce enzymes such as amylase, protease, lipase, cellulose, but unable to produce galactosidase. The isolate can’t produce gelatinase and DNase. The isolate showed moderate anti-microbial activity against bacteria and fungi and cell lysate showed better antimicrobial activity than whole cell and culture supernatant. Again, the isolate showed better anti-bacterial activity against gram negative bacteria than gram positive. The isolate showed strong antioxidant activity, reducing power, nitric oxide and hydroxyl radical scavenging activity, significant brine shrimp cytotoxicity and acute toxicity and metal ion chelating activity. The isolate did not induce any detectable change in general health of mice upon oral toxicity testing and found to be safe in mouse model. The isolate improve lymphocyte proliferation and cytokine production in treated mice.ConclusionsSuch isolate could be potential as probiotic to be used therapeutically.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Fakruddin

Hossain

Ahmed

2017

BMC Complement Altern Med

147

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“…The yeast Saccharomyces cerevisiae is an organism that is able to grow at low pH values (38), although it does not naturally produce fumaric acid. Additional advantages of this organism are that its genome has been fully sequenced and that it is considered by the American Food and Drug Administration (FDA) as an organism generally regarded as safe (GRAS) (6). In addition, in several recent studies, the yeast S. cerevisiae has been proposed as a cell factory for the production of bulk chemicals, such as malic, lactic, and citric acid from renewable substrates (1,30,36,42,43).…”

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confidence: 99%

pH-Dependent Uptake of Fumaric Acid in Saccharomyces cerevisiae under Anaerobic Conditions

Jamalzadeh

Verheijen

Heijnen

et al. 2012

Appl Environ Microbiol

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Microbial production of C 4 dicarboxylic acids from renewable resources has gained renewed interest. The yeast Saccharomyces cerevisiae is known as a robust microorganism and is able to grow at low pH, which makes it a suitable candidate for biological production of organic acids. However, a successful metabolic engineering approach for overproduction of organic acids requires an incorporation of a proper exporter to increase the productivity. Moreover, low-pH fermentations, which are desirable for facilitating the downstream processing, may cause back diffusion of the undissociated acid into the cells with simultaneous active export, thereby creating an ATP-dissipating futile cycle. In this work, we have studied the uptake of fumaric acid in S. cerevisiae in carbon-limited chemostat cultures under anaerobic conditions. The effect of the presence of fumaric acid at different pH values (3 to 5) has been investigated in order to obtain more knowledge about possible uptake mechanisms. The experimental results showed that at a cultivation pH of 5.0 and an external fumaric acid concentration of approximately 0.8 mmol · liter ؊1 , the fumaric acid uptake rate was unexpectedly high and could not be explained by diffusion of the undissociated form across the plasma membrane alone. This could indicate the presence of protein-mediated import. At decreasing pH levels, the fumaric acid uptake rate was found to increase asymptotically to a maximum level. Although this observation is in accordance with proteinmediated import, the presence of a metabolic bottleneck for fumaric acid conversion under anaerobic conditions could not be excluded.

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“…Jüngste Aufmerksamkeit gilt der L-Milchsäure als Ausgangsmonomer für den biologisch abbaubaren Kunststoff Polylactid (PLA). Aufgrund des wachsenden Marktes für Polymilchsäure und der Erschließung neuer Einsatzmöglichkeiten wird die derzeitige Produktion von etwa 250 000 Jahrestonnen Milchsäure deutlich steigen [4]. Seit 1895 wird MS überwiegend biotechnologisch hergestellt, da die MS annähernd optisch rein produziert werden kann, was für die Dilactidsynthese zur Produktion von PLA von Bedeutung ist.…”

Section: Einleitung Und Problemstellungunclassified

Fermentative Herstellung von L‐Lysin‐L‐lactat mittels Fraktionierungssäften aus der Grünen Bioraffinerie

Leiß

Kamm

Venus

2010

Chemie Ingenieur Technik

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Metabolically Engineered Yeasts: ‘Potential’ Industrial Applications

Abstract: Industrial biotechnology and metabolic engineering can offer an innovative approach to solving energy and pollution problems. The potential industrial applications of yeast are reviewed here.

Cited by 27 publications

References 94 publications

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

pH-Dependent Uptake of Fumaric Acid in Saccharomyces cerevisiae under Anaerobic Conditions

Fermentative Herstellung von L‐Lysin‐L‐lactat mittels Fraktionierungssäften aus der Grünen Bioraffinerie

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