Metabolism and accumulation of hydroxylated fatty acids by castor (Ricinus comunis) seed microsomes

Sánchez-Álvarez, A.J.; Ruiz-López, Noemı́; Moreno‐Pérez, Antonio J.; Venegas-Calerón, Mónica; Martı́nez-Force, Enrique; Garcés, Rafael; Salas, Joaquı́n J.

doi:10.1016/j.plaphy.2021.12.010

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…Es un ácido graso hidroxilado con una estructura única que lo distingue de otros ácidos grasos. El aceite de ricino se utiliza en diversas aplicaciones industriales, como lubricantes, productos químicos y cosméticos debido a sus propiedades especiales o otros procesos industriales, (Dimian, 2017), (Baque, et al 2022), (Sánchez-Álvarez et al, 2022), (Xu et al, 2023).…”

Section: Introductionunclassified

Evaluación en el proceso químico del aceite y biodiesel del Ricinus communis L. de la variedad INIAP 402. Lodana-Manabí.

Mendoza Zambrano,

García Muentes,

Saltos Giler

et al. 2023

Rev. Cient. Multi. G-nerando

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El presente trabajo de investigación se realizó en los laboratorios de química de la UTM e INIAP, con la utilización de semillas higuerilla en la determinación de la composición química del aceite y biodiesel del Ricinus communis L. variedad INIAP 402. La metodología implementada es investigativa-practica en el área química, la determinación de los perfiles de los ácidos grasos, se realizó por comparación de los tiempos de retención de los picos con sus respectivos estándares y la cuantificación por el método Villavicencio, 2001 y AOAC 996.06, el extracto orgánico que contiene los esteres de ácidos grasos se concentra a sequedad con corriente de nitrógeno, la separación se realiza en una columna capilar SUPELCO SP TM 2560 de 100 m*0,25mm*0,2μm, utilizando una programación de temperatura del horno de columna. Los resultados se expresan como gramos de cada ácido graso por cada 100 g de grasa de la higuerilla variedad INIAP 402, el contenido de aceite es 52,2%, índice de acides 2,76 mg KOH*g-1, Viscosidad Cinemática 203,2 mm2*s-1, Densidad Relativa 957,6Kg*m-3, Índice de Peróxidos 0.5≤Meq de peróxido*Kg-1 muestra, Índice de Iodo 87,98g IODO*100g-1, Humedad 646,5 ppm, Índice de Saponificación 171,83 mmg KOH*g-1, la determinación ácidos grasos del Ricinus communis L. variedad INIAP 402 fueron; Palmítico 1,52%, Esteárico 1,57%, Elaídico 31,29%, Oleico 3,7%, Linoleico 3,05%, Ricinoleico 84,1%, Palmitoleico 0,11%, Heptadecanoico 0,17%, Linolelaídico 0,67%, Araquídico 0,62%, linoleico 1,9%, Heneicosanoico 1,36%, Eicosadienoico 0,74%, Eruci 0,25%, Tricosanoico 0,24% y para el biodiesel no hay diferencias significativas en los porcentajes.

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Section: Introductionunclassified

Evaluación en el proceso químico del aceite y biodiesel del Ricinus communis L. de la variedad INIAP 402. Lodana-Manabí.

Mendoza Zambrano,

García Muentes,

Saltos Giler

et al. 2023

Rev. Cient. Multi. G-nerando

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“…Castor (Ricinus communis L.) is an annual plant in the genus Ricinus family Euphorbiaceae and is principally found in tropical or subtropical regions. Castor seeds contains high oil content (about 46-56%) and are a raw material for making excellent industrial oils (Sánchez-Álvarez et al, 2019). Castor oil has high temperature resistance and high viscosity and is difficult to solidify (Sbihi, Nehdi, Mokbli, Romdhani-Younes,…”

Section: Introductionmentioning

confidence: 99%

In vitro grafting procedure for facilitating growth of separated shoots in castor

Liu

et al. 2021

Agronomy Journal

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Regenerated adventitious buds in an Agrobacterium-mediated genetic transformation system of castor (Ricinus communis L.) are difficult to develop in intact plants.Therefore, an in vitro grafting system in castor was established in this study. The stocks and scions were obtained from castor seedlings, and complete castor plants could be gained by in vitro grafting. To promote growth of grafted seedlings, the effects of indole 3-butyric acid (IBA), 6-benzyl aminopurine (6-BA), kinetin (KT), sodium nitrophenolate (SN), and diethyl aminoethyl hexanoate (DA-6) on the development of grafted seedlings were explored. The experimental results indicated that when adding IBA, KT, and SN to the media at concentrations of 0.1, 0.2, and 0.8 mg L −1 , respectively, the growth of castor grafted seedlings could be promoted strongly; however, when 6-BA or DA-6 was added to media, the growth and development of castor grafted plantlet were inhibited significantly. Moreover, the survival rate of transplantation of grafted seedlings was up to 74.5%. The method of in vitro grafting in castor could be used to obtain robust and complete castor plants quickly and might provide a solution to the problems of adventitious bud growing and rooting difficulty in the genetic transformation of castor.

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ACAD10 and ACAD11 allow entry of 4-hydroxy fatty acids into β-oxidation

Paquay,

Duraffourd,

Bury

et al. 2024

Cell. Mol. Life Sci.

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Hydroxylated fatty acids are important intermediates in lipid metabolism and signaling. Surprisingly, the metabolism of 4-hydroxy fatty acids remains largely unexplored. We found that both ACAD10 and ACAD11 unite two enzymatic activities to introduce these metabolites into mitochondrial and peroxisomal β-oxidation, respectively. First, they phosphorylate 4-hydroxyacyl-CoAs via a kinase domain, followed by an elimination of the phosphate to form enoyl-CoAs catalyzed by an acyl-CoA dehydrogenase (ACAD) domain. Studies in knockout cell lines revealed that ACAD10 preferentially metabolizes shorter chain 4-hydroxy fatty acids than ACAD11 (i.e. 6 carbons versus 10 carbons). Yet, recombinant proteins showed comparable activity on the corresponding 4-hydroxyacyl-CoAs. This suggests that the localization of ACAD10 and ACAD11 to mitochondria and peroxisomes, respectively, might influence their physiological substrate spectrum. Interestingly, we observed that ACAD10 is cleaved internally during its maturation generating a C-terminal part consisting of the ACAD domain, and an N-terminal part comprising the kinase domain and a haloacid dehalogenase (HAD) domain. HAD domains often exhibit phosphatase activity, but negligible activity was observed in the case of ACAD10. Yet, inactivation of a presumptive key residue in this domain significantly increased the kinase activity, suggesting that this domain might have acquired a regulatory function to prevent accumulation of the phospho-hydroxyacyl-CoA intermediate. Taken together, our work reveals that 4-hydroxy fatty acids enter mitochondrial and peroxisomal fatty acid β-oxidation via two enzymes with an overlapping substrate repertoire.

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Metabolism and accumulation of hydroxylated fatty acids by castor (Ricinus comunis) seed microsomes

Cited by 5 publications

References 35 publications

Evaluación en el proceso químico del aceite y biodiesel del Ricinus communis L. de la variedad INIAP 402. Lodana-Manabí.

Evaluación en el proceso químico del aceite y biodiesel del Ricinus communis L. de la variedad INIAP 402. Lodana-Manabí.

In vitro grafting procedure for facilitating growth of separated shoots in castor

ACAD10 and ACAD11 allow entry of 4-hydroxy fatty acids into β-oxidation

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