Metabolism and aging in the filamentous fungus Podospora anserina

Osiewacz, Heinz D.; Stumpferl, Stefan W.

doi:10.1016/s0167-4943(01)00096-6

Cited by 31 publications

(11 citation statements)

References 46 publications

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“…Observation of similar results also for F. acuminatum indicates that SOD and CAT were sufficient to protect the Fusarium strain cells against the potentially deleterious effects of ROS [39]. After the 12th day, LPO levels besides intracellular iron and manganese levels significantly increased while SOD and CAT activities decreased.…”

Section: Discussionsupporting

confidence: 61%

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Variations in metal uptake, antioxidant enzyme response and membrane lipid peroxidation level in Fusarium equiseti and F. acuminatum

Kayalı

Tarhan

2005

Process Biochemistry

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Section: Discussionsupporting

confidence: 61%

“…After the 12th day, LPO levels besides intracellular iron and manganese levels significantly increased while SOD and CAT activities decreased. These results may indicate that permeability of membrane becomes more damaged and therefore higher amounts of ROS are generated as a result of aging [39].…”

Section: Discussionmentioning

confidence: 98%

Variations in metal uptake, antioxidant enzyme response and membrane lipid peroxidation level in Fusarium equiseti and F. acuminatum

Kayalı

Tarhan

2005

Process Biochemistry

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“…Respiratory function is also a key factor in controlling the life-span of the filamentous fungus Podospora anserina. Several mutants of this organism, all related to the cytochrome pathway, displayed a strikingly increased longevity and have been described thoroughly (Borghouts et al, 2001(Borghouts et al, , 2002aDufour et al, 2000;Lorin et al, 2006Lorin et al, , 2001Osiewacz, 2002;Osiewacz & Stumpferl, 2001;Sainsard-Chanet & Begel, 1990;Schulte et al, 1989). The phenotypic effects were traced back to almost exclusive respiration via the alternative pathway, and elevated levels of the alternative oxidase (PaAox) protein were found.…”

Section: Discussionmentioning

confidence: 98%

Pichia pastoris ‘just in time’ alternative respiration

et al. 2007

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Alternative oxidases (Aox or Aod) are present in the mitochondria of plants, fungi and many types of yeast. These enzymes transfer electrons from the ubiquinol pool directly to oxygen without contributing to the proton transfer across the mitochondrial membrane. Alternative oxidases are involved in stress responses, programmed cell death and maintenance of the cellular redox balance. The alternative oxidase gene of the methylotrophic yeast Pichia pastoris was isolated and cloned to study its regulation and the effects of deregulation of the alternative respiration by overexpression or disruption of the gene. Both disruption and overexpression had negative effects on the biomass yield; however, the growth rate and substrate uptake rate of the strain overexpressing the alternative oxidase were slightly increased. These effects were even more pronounced when higher glucose concentrations were used. The occurrence of free intracellular radicals and cell death phenomena was investigated using dihydrorhodamine 123 and the TUNEL test. The results suggest a major contribution of the alternative oxidase to P. pastoris cell viability. The negative effects of deregulated alternative respiration clearly indicated the importance of precise regulation of the alternative oxidase in this yeast.

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“…Moreover, the incidence of mismatches could be high in the mtDNAs of cytochrome-deWcient mutants (stp-ruv, cyt-5, cyt-23, cyt-24, etc. ) due to oxidative damage to pyrimidines and purines resulting from the presumptively enhanced production of reactive oxygen species in their mitochondria (reviewed by Osiewacz and Stumpferl, 2001). In this context, it is relevant to note that a review of the folded structures of the subset of GC-rich imperfect palindromes that were involved in the generation of large deletions in the mtDNA of the E35 mitochondrial stopper mutant (De Vries et al, 1986;Almasan and Mishra, 1988, 1991Mishra, 1991) revealed that all the pertinent mtDNA breaks also could have occurred at points immediately adjacent to unpaired nucleotides (data not shown).…”

Section: Generation Of Plmesmentioning

confidence: 99%