Metabolism-Based Herbicide Resistance and Cross-Resistance in Crop Weeds: A Threat to Herbicide Sustainability and Global Crop Production

Yu, Qin; Powles, Stephen B.

doi:10.1104/pp.114.242750

Cited by 385 publications

(461 citation statements)

References 115 publications

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“…However, for B 13 biotype, the application of iodosulfuron herbicide after the use of malathion as a P 450 inhibitor resulted in 21% control, in contrast to the other treatments (Table 3). The use of the cytochrome P 450 inhibitor in Lolium rigidum (Gaudin) biotypes before clorosulfuron herbicide showed a synergistic effect compared to the isolated application of the herbicide (Yu & Powles, 2014). However, spraying imazethapyr herbicide either alone or in combination with PBO or malathion resulted in less than 2% control, suggesting that there is no differential metabolism by cytochrome P 450 monooxygenase in these biotypes.…”

Section: Metabolizationmentioning

confidence: 94%

<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

et al. 2017

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ABSTRACT. Acetolactate synthase inhibitors are the main group of herbicides used in winter crops in Southern Brazil where their intensive use has selected for herbicide-resistant biotypes of radish. The resistance affects the efficacy of herbicides, and identifying the resistance mechanism involved is important for defining management strategies. The aim of this study was to elucidate the resistance mechanism of radish biotypes by quantifying the enzyme activity, ALS gene sequencing and evaluating the response of biotypes to iodosulfuron and imazethapyr herbicide application after treatment with a cytochrome P 450 monooxygenase inhibitor. The susceptible (B 1 ) and resistant (B 4 and B 13 ) biotypes were from wheat fields in the Northwest of Rio Grande do Sul State. The results demonstrated that the enzyme affinity for the substrate (K M ) was not affected in biotypes B 4 and B 13 but that the V max of the resistant biotypes was higher than that of biotype B 1 . The resistant biotypes showed no differential metabolic response to iodosulfuron and imazethapyr herbicides when inhibited by malathion and piperonyl butoxide. However, gene sequencing of ALS showed a mutation at position 574, with an amino acid substitution of tryptophan for leucine (Trp-574-Leu) in resistant biotypes.Keywords: Raphanus sativus, mechanism of resistence, ALS enzyme activity, gene mutation, metabolism.Mutação da Trp-574-Leu do gene ALS confere resistência de biótipos de nabo ao herbicida iodosulfurom e imazetapir RESUMO. Os inibidores da acetolactato sintase são o principal grupo de herbicidas usados em culturas de inverno do Sul do Brasil onde seu uso intenso selecionou biótipos resistentes de nabo. A resistência afeta a eficácia dos herbicidas, e a identificação do mecanismo de resistência envolvido é importante na definição de estratégias de manejo. O objetivo deste estudo foi elucidar o mecanismo de resistência em biótipos de nabo através da quantificação da atividade da enzima, sequenciamento do gene ALS e avaliar a resposta dos biótipos à aplicação do herbicida iodosulfurom e imazetapir após tratamento com inibidores do metabolismo da citocromo P 450 monooxigenase. Os biótipos suscetível (B 1 ) e resistentes (B 4 e B 13 ) eram de lavouras de trigo da região Noroeste do Estado do Rio Grande do Sul. Os resultados demonstraram que a afinidade da enzima pelo substrato (K M ) não foi afetada nos biótipos B 4 e B 13 , porém o V max dos biótipos resistentes foi superior quando comparado ao biótipo B 1 . Os biótipos resistentes não apresentaram resposta metabólica diferencial ao herbicida iodosulfurom e imazetapir quando inibidos pelo malathion e butóxido de piperolina. Entranto, o sequenciamento do gene ALS evidenciou mutação na posição 574 com uma substituição do aminoácido triptofano por leucina (Trp-574-Leu) nos biótipos resistentes. Palavras-chave:Raphanus sativus, mecanismo de resistência, atividade da enzima ALS, mutação gênica, metabolismo.

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Section: Metabolizationmentioning

confidence: 94%

<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

et al. 2017

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“…The use of half the maximum registered dose is justified by the fact that the process of resistance selection may be influenced by the use of sub doses of herbicides and because there is differential susceptibility of biotypes to the application of the herbicide (Neve & Powles, 2005;Yu et al, 2013;Yu & Powles, 2014).…”

Section: Resultsmentioning

confidence: 99%

Geographic Distribution of Ryegrass Resistent to the Clethodim Herbicide in Rio Grande do Sul1

et al. 2016

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-Ryegrass is a weed of annual cycle that is present in winter crops, in orchards and vineyards of the South region of Brazil. The species is normally controlled by the glyphosate herbicide, but the continuous use of this product caused the selection of resistant biotypes. The use of ACCase inhibitor herbicides is the main alternative for the control of this species, but it has not been satisfactory in some places, thus causing suspicion of resistance to this action mechanism. Thus, the objective of this paper was to evaluate the occurrence and geographic distribution of ryegrass biotypes that are resistant to the clethodim herbicide in the state of Rio Grande do Sul. For that, seeds of ryegrass plants that survived the application of clethodim were collected in crops from the north region of RS, summing up to a total of 152 samples from 72 cities. The biotypes were submitted to the application of 120 g i.a. ha -1 (maximum registered dose) and 60 g i.a. ha -1 de clethodim (half the maximum registered dose). According to the results, among the samples of ryegrass seeds collected, there were no biotypes resistant to the clethodim herbicide when the maximum registered dose was applied and in the stage of three to four leaves. However, there were biotypes with lower susceptibility that survived half the maximum registered dose.

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“…Following widespread applications of ACCase-inhibiting herbicides, resistance to these graminicides developed [4]. In cereal and leguminous grain crop areas of South Australia, a large number of populations of annual ryegrass (Lolium rigidum) have developed resistance to diclofop-methyl following selection with this herbicide.…”

Section: Metabolism and Selectivity Of Accase Inhibitor Aryloxyphenoxmentioning

confidence: 99%

“…On the other hand, repeated use of herbicides with similar chemistry may lead to the selection of herbicide-resistant biotypes with an enhanced capacity to degrade herbicides. Weed species that have evolved resistance to herbicides due to enhanced metabolic capacity have been a major issue [4]. Target-site resistance develops by mutation within a gene coding for an herbicide target-site enzyme or by overproduction of the target enzyme.…”

Section: Introductionmentioning

confidence: 99%

Herbicide Metabolism in Weeds — Selectivity and Herbicide Resistance

Jablonkai¹

2015

Herbicides, Physiology of Action, and Safety

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The metabolic detoxication/bioactivation pathways, the levels and activity of enzymes, and endogenous cofactors mediating these reactions in crops have been well documented; however, much less evidence has been accumulated in weed species. The herbicide metabolism as a selectivity factor is summarized with special attention to acetyl-CoA carboxylases (ACCase)-inhibiting aryloxyphenoxypropionate, protoporphyrinogen IX oxidase (PPO) inhibitor, carotenoid biosynthesis inhibitor clomazone, and acetolactate synthase (ALS) inhibitor imidazolinone and sulfonylurea herbicides in various weed species. The metabolism-based herbicide resistance related to these herbicide classes is also discussed along with the role and level of metabolizing enzymes and cofactors in weed species.

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Metabolism-Based Herbicide Resistance and Cross-Resistance in Crop Weeds: A Threat to Herbicide Sustainability and Global Crop Production

Cited by 385 publications

References 115 publications

<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

Geographic Distribution of Ryegrass Resistent to the Clethodim Herbicide in Rio Grande do Sul1

Herbicide Metabolism in Weeds — Selectivity and Herbicide Resistance

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