Metabolism of 6-benzyladenine by leaf explants of Lachenalia during adventitious bud formation

Niederwieser, J. G.; Staden, J. Van; Upfold, S. J.; Drewes, F. E.

doi:10.1016/s0254-6299(16)30840-7

Cited by 10 publications

(4 citation statements)

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“…Highest number of buds (20.6) in Lachenalia cultures were recorded on lower BA concentration (0.5 and 1.0 mg L −1 ) [15]. Additionally, 1.0 mg L −1 BA with 0.1 mg L −1 NAA occurred to be the best combination for bulbing in Lachenalia 'Romaud' culture [28]. High level of BA or NAA inhibits formation of bulbs in Lilium cultures as well [53,54], however, slight differences were observed on Lilium regale scale explants, between 5 µM and 10 µM BA treatment in bulbing, which amounted to 1.7 and 2.4 bulbs, respectively [27].…”

Section: Resultsmentioning

confidence: 86%

“…Most Lachenalia species form not enough daughter bulbs [4], thus high regeneration potential of leaf tissue within this genus [14] is used by growers in commercial lachenalia propagation via leaf cuttings [49]. Leaf explants were also most often applied during in vitro organogenesis within this genus [11,15,28]. In the present experiment, bulb explants (the counterpart of a modified leaf) were also used with sufficient results, whereas callus explant, probably due to its structure and characteristics, turned out to be a worse plant material, although it was used successfully in an experiment with others geophytes [50,51].…”

Section: Resultsmentioning

confidence: 99%

“…Proper type and concentration of PGRs are essential to develop efficient clonal regeneration protocol for Lachenalia viridiflora. PGRs like: BA (cytokinin) and NAA (auxin) are routinely applied to induce various geophytes' organogenesis [20,[22][23][24][25][26][27], also in lachenalia propagation [9,15,18,28]. According to Aremu et al (2017) [11], BA belongs to the best cytokinin for propagation of Lachenalia montana.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Organogenesis of Critically Endangered Lachenalia viridiflora

2022

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Micropropagation via organogenesis of Lachenalia viridiflora W. F. Barker, a native endangered plant from South Africa, is presented in this study. Leaves, collected from in vivo plants, were a source for culture initiation to obtain three types of explants (leaf, bulb, callus). They were grown on Murashige and Skoog (MS) medium supplemented with two concentrations of sucrose (3 or 6%, marked as S3 and S6) and different concentrations of benzylaminopurine (BA) (0.0–5.0 μM) and naphthalene acetic acid (NAA) (0.0–0.5 μM). The results demonstrated that bulbing was more efficient on media with 3% sucrose and this process was closely related to explant type—irrespective of media type twice more adventitious bulbs (5.1–5.6) were regenerated on leaf and bulb explants than on callus (2.6). The highest value of adventitious bulb number was recorded for interaction: leaf × S3/2.5 BA/0.5 NAA (9.3 bulbs per explant). Irrespective of media type, the higher the number of adventitious bulbs (obtained from leaf and bulb explants), the lower their diameter. Moreover, adventitious bulbs regenerated from bulb explants formed the longest leaves (18.9 mm) and in the greatest percentage (52.3%). The highest biomass weight increase indicator (50 times) was recorded for interaction: leaf × S6/2.5 BA/0.25 NAA. Various combinations of plant growth regulators favor biomass increase.

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Organogenesis of Critically Endangered Lachenalia viridiflora

2022

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“…The importance of metabolites within leaf tissue on the growth of buds once formed, has been demonstrated by the observation that the number of buds on explants is independent of explant size, whereas the growth of these buds is better on large than on small explants (Niederwieser a nd Vcelar, 1990). Van Staden (1990a and1992b) did some preliminary studies on cytokinin levels and bud formation. No relationship was found between the level of endogenous cytokinin and the regeneration potential as determined by the genotype.…”

Section: Origin Of Adventitious Budsmentioning

confidence: 99%

Review on Adventitious Bud Formation in Lachenalia

Niederwieser¹,

Ndou²

2002

Acta Hortic.

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Lachenalia varieties are propagated vegetatively through adventitious bud formation. In this report, published and unpublished results and observations are reviewed. Adventitious buds are formed on leaves, peduncles, bulb-scales and axillary meristems. In leaf tissue, buds form from epidermal cells and more than 90% originate from single cells. The regeneration potential of species and varieties varies amongst species and this factor thus became an important evaluation criterion in the breeding programme. Tissue age affects both the number of buds formed, as well as the size of bud/bulblet. Young tissue proved to be the best source of tissue in several studies. The physiological stage of the mother plant affected bud formation on bud forming tissue. Once plants enter the post anthesis stage, the regeneration potential decreases markedly. This effect is less evident when plant growth regulators (PGR's) are used in vitro. PGR's stimulate bud formation markedly and an interaction between the regeneration potential, tissue age and the optimal PGR concentration was shown. Propagation through adventitious bud formation will continue to play an important role in the production of the crop as lachenalias are not resistant to virus diseases. Growers therefore have to adhere to a propagation scheme with different phases and limited generations in each phase.

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The chemical microenvironment

Williams

1995

Automation and Environmental Control in Plant Tissue Culture

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Metabolism of 6-benzyladenine by leaf explants of Lachenalia during adventitious bud formation

Cited by 10 publications

References 10 publications

In Vitro Organogenesis of Critically Endangered Lachenalia viridiflora

In Vitro Organogenesis of Critically Endangered Lachenalia viridiflora

Review on Adventitious Bud Formation in Lachenalia

The chemical microenvironment

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