Metabolism of Antarctic micronektonic crustacea across a summer ice-edge bloom: respiration, composition, and enzymatic activity

Donnelly, Joseph; Kawall, Helena G.; Geiger, Stephen P.; Torres, Joseph J.

doi:10.1016/j.dsr2.2004.07.003

Cited by 20 publications

(15 citation statements)

References 40 publications

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“…3). The activity of enzymes malate dehydrogenase (MDH) and lactate dehydrogenase (LDH), proxies for overall metabolism and respiration [85]–[87], were consistently higher and protein content was consistently lower in the high CO 2 treatment compared to ambient (Fig. 4).…”

Section: Resultsmentioning

confidence: 99%

Increased Feeding and Nutrient Excretion of Adult Antarctic Krill, Euphausia superba, Exposed to Enhanced Carbon Dioxide (CO2)

et al. 2012

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Ocean acidification has a wide-ranging potential for impacting the physiology and metabolism of zooplankton. Sufficiently elevated CO2 concentrations can alter internal acid-base balance, compromising homeostatic regulation and disrupting internal systems ranging from oxygen transport to ion balance. We assessed feeding and nutrient excretion rates in natural populations of the keystone species Euphausia superba (Antarctic krill) by conducting a CO2 perturbation experiment at ambient and elevated atmospheric CO2 levels in January 2011 along the West Antarctic Peninsula (WAP). Under elevated CO2 conditions (∼672 ppm), ingestion rates of krill averaged 78 µg C individual−1 d−1 and were 3.5 times higher than krill ingestion rates at ambient, present day CO2 concentrations. Additionally, rates of ammonium, phosphate, and dissolved organic carbon (DOC) excretion by krill were 1.5, 1.5, and 3.0 times higher, respectively, in the high CO2 treatment than at ambient CO2 concentrations. Excretion of urea, however, was ∼17% lower in the high CO2 treatment, suggesting differences in catabolic processes of krill between treatments. Activities of key metabolic enzymes, malate dehydrogenase (MDH) and lactate dehydrogenase (LDH), were consistently higher in the high CO2 treatment. The observed shifts in metabolism are consistent with increased physiological costs associated with regulating internal acid-base equilibria. This represents an additional stress that may hamper growth and reproduction, which would negatively impact an already declining krill population along the WAP.

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Section: Resultsmentioning

confidence: 99%

Increased Feeding and Nutrient Excretion of Adult Antarctic Krill, Euphausia superba, Exposed to Enhanced Carbon Dioxide (CO2)

et al. 2012

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“…In fact, however, large among-species variations in water content (% of WM), C and N (both % of DM) have been reported. These values (water content, C and N) range from 74.0 to 92.8, from 31.6 to 57.8 and from 6.4 to 14.0, respectively, for 15 pelagic cephalopods (all squids; Clarke et al, 1985;Ikeda and Bruce, 1986;Donnelly et al, 2004;Ikeda, unpublished data) and from 63.6 to 93.9, from 18.4 to 55.6 and from 3.5 to 16.0, respectively, for 58 pelagic fishes (Whitledge and Packard, 1971;Torres et al, 1979;Torres and Somero, 1988;Donnelly et al, 1999;Bishop et al, 2000;Ikeda et al, 2011). In the present analyses, body mass was expressed as WM, DM, C or N to examine the effects of body mass units on the results and for meaningful between-taxon metabolic comparison (Zeuthen, 1947;Schneider, 1990;Ikeda, 2008;Acuña et al, 2011).…”

Section: The Metabolic Datamentioning

confidence: 99%

Routine metabolic rates of pelagic marine fishes and cephalopods as a function of body mass, habitat temperature and habitat depth

Ikeda¹

2016

Journal of Experimental Marine Biology and Ecology

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Routine metabolic rates of pelagic marine fishes and cephalopods as a function of body mass, habitat temperature and habitat depth Tsutomu Ikeda 16-3-1001 Toyokawa-cho, Hakodate 040-0065, Japan a b s t r a c t Cephalopods and fishes are major components of marine micronekton and nekton, so an understanding of their physiology and roles in ocean biogeochemistry is important. I compiled the routine respiration rates (50 datasets on 41 cephalopod species; 102 datasets on 90 fish species) from various depth horizons (b1300 m) of the world's oceans and analyzed these rates as a function of body mass [wet mass (WM), dry mass (DM), carbon (C) or nitrogen (N)], habitat temperature and habitat depth using multiple regression. Stepwise-regression analyses revealed that body mass was the most important parameter, followed by habitat temperature and habitat depth, and these variables explained 89.7-93.8% and 94.7-95.8%, respectively, of the variance in the respiration data of fishes and cephalopods. The addition of a taxon category (order or family) as a fourth variable improved these correlations only slightly (95.6-95.7% and 95.7-96.2%, respectively). The resultant regression equation showed higher respiration rates in cephalopods than fishes relative to the DM, C or N body mass (by a factor 1.5-to 1.7-fold), but not to the WM body mass. The O:N ratios (respiration/ammonia excretion, by atoms) reported for 6 cephalopods (median: 13.2) and 35 fishes (24.2) suggested the predominance of protein as a metabolite in the former and carbohydrate or lipid in the latter. The present results are discussed in light of the methodological constraints and standing hypothesis for the relationship between the metabolic rate and temperature. The empirical models established in the present study can be used to assess the roles of cephalopods and fishes in C and N cycles in pelagic ecosystems based on the organisms' body mass spectra, ambient temperatures and depth distributions.

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“…The lack of significance in all correlations for respiration rate does not imply that biochemical indices cannot be used for assessing respiration. For example, Donnelly et al (2004) investigated several biochemical indices in a variety of crustaceans and found that protein, RNA and DNA contents, and their ratio were significantly related to respiration. In their study, however, a large variability in the predictive capacity of these indices was observed among different species, ranging from nonsignificant to highly significant.…”

Section: Discussionmentioning

confidence: 99%

Biochemical proxies for growth and metabolism in Acartia bifilosa (Copepoda, Calanoida)

Holmborn

Dahlgren

Holeton

et al. 2009

Limnology & Ocean Methods

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Biochemical proxies are becoming increasingly common for growth assessment in zooplankton. Their suitability is often unknown, however, and proper calibration is lacking. We investigated correlations between physiological variables (ingestion, egg production, and respiration rates) and biochemical indices related to protein synthesis (RNA content, RNA:DNA ratio, RNA:protein ratio, and protein specific aminoacyl-tRNA synthetases [spAARS] activity) in copepods Acartia bifilosa exposed to different algal concentrations (0-1200 µg C L -1). All variables assayed increased with increasing food concentration either linearly (spAARS) or nonlinearly (all other variables). Egg production and ingestion rates were significantly and positively correlated with RNA content and RNA:protein ratio, whereas correlations with spAARS and RNA:DNA ratio were weaker or nonsignificant. However, when RNA:DNA ratio and spAARS activity were used as predictors of ingestion, together they had higher explanatory value than did either variable separately. As there were substantial differences in saturating food concentrations among the assayed variables, applicability of biomarkers as proxies of physiological rates will be more reliable if restricted to the nonsaturated phase of the functional response of either variable, unless both variables saturate simultaneously. These findings contribute to methodology of zooplankton growth assessment and to our understanding of biochemical processes underlying growth and metabolism in copepods.

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Metabolism of Antarctic micronektonic crustacea across a summer ice-edge bloom: respiration, composition, and enzymatic activity

Cited by 20 publications

References 40 publications

Increased Feeding and Nutrient Excretion of Adult Antarctic Krill, Euphausia superba, Exposed to Enhanced Carbon Dioxide (CO2)

Increased Feeding and Nutrient Excretion of Adult Antarctic Krill, Euphausia superba, Exposed to Enhanced Carbon Dioxide (CO2)

Routine metabolic rates of pelagic marine fishes and cephalopods as a function of body mass, habitat temperature and habitat depth

Biochemical proxies for growth and metabolism in Acartia bifilosa (Copepoda, Calanoida)

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