Metabolism of Biotin and Oxybiotin by
            <i>Lactobacillus pentosus</i>
            124-2

Krueger, Keatha K.; Peterson, W. H.

doi:10.1128/jb.55.5.693-703.1948

Cited by 34 publications

(8 citation statements)

References 21 publications

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“…For M. echinata and S. atra desthiobiotin is inactive, but combined with biotin it has the same growth-promoting activity as an amount of biotin equal to the total weight of the mixture (Perlman, 1948a). The biotin activity of 0-heterobiotin has been demonstrated for Lactobacillus arabinosus, L. casei, Saccharomyces cerevisae, R. trifolii (Winnick et al, 1945;Pilgrim et al, 1945;Rubin, Flower, et al, 1945;Duschinsky et al, 1945), for clostridia (Perlman, 1948b;Shull and Peterson, 1948), for M. echinata and S. atra (Perlman, 1948a), and for L. pentosus (Krueger and Peterson, 1948).…”

Section: Discussionmentioning

confidence: 94%

“…This analog has been shown to act as an inhibitor to both biotin and 0-heterobiotin for L. casei (Dittmer and du Vigneaud, 1944; Lilly and Leonian, 1944;Tatum, 1945;Rubin, Drekter, and Moyer, 1945;Rubin, Flower, et al, 1945). However, desthiobiotin is inactive for Lactobacillus arabino8us, Lactobacillus pentosus, and Rhizobium trifolii either when alone or when biotin is present (Lilly and Leonian, 1944;Krueger and Peterson, 1948). For M. echinata and S. atra desthiobiotin is inactive, but combined with biotin it has the same growth-promoting activity as an amount of biotin equal to the total weight of the mixture (Perlman, 1948a).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Studies on the Vitamin Nutrition of Allescheria Boydii Shear

Villela

Cury

1950

J Bacteriol

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Studies on the Vitamin Nutrition of Allescheria Boydii Shear

Villela

Cury

1950

J Bacteriol

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“…Other investigators have shown that bacteria and yeast remove biotin from various growth media (9,15,18), but in such studies the mechanism of biotin transport was not determined. The present work was initiated to characterize the biotin transport process in Saccharomyces cerevisiae.…”

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confidence: 99%

Characterization of the Biotin Transport System in Saccharomyces cerevisiae

Rogers

Lichstein

1969

J Bacteriol

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The characteristics of the biotin transport mechanism of Saccharomyces cerevisiae were investigated in nonproliferating cells. Microbiological and radioisotope assays were employed to measure biotin uptake. The vitamin existed intracellularly in both free and bound forms. Free biotin was extracted by boiling water. Chromatography of the free extract showed it to consist entirely of d-biotin. Cellular bound biotin was released by treating cells with 6 N H2SO4. The rate of biotin uptake was linear with time for 10 min, reaching a maximum at about 20 min followed by a gradual loss of accumulated free vitamin from the cells. Biotin was not degraded or converted to vitamers during uptake. Transport was temperature-and pH-dependent, optimum conditions for uptake being 30 C and pH 4.0. Glucose markedly stimulated biotin transport. In its presence, large intracellular free-biotin concentration gradients were established. Iodoacetate inhibited the glucose stimulation of biotin uptake. The rate of vitamin transport increased in a linear fashion with increasing cell mass. The transport system was saturated with increasing concentrations of the vitamin. The apparent Km for uptake was 3.23 X 10-7 M. Uptake of radioactive biotin was inhibited by unlabeled biotin and a number of analogues including homobiotin, desthiobiotin, oxybiotin, norbiotin, and biotin sulfone. Proline, hydroxyproline, and 7,8-diaminopelargonic acid did not inhibit uptake. Unlabeled biotin and desthiobiotin exchanged with accumulated intracellular "4C-biotin, whereas hydroxyproline did not. 1 Presented in part at the 68th Annual Meeting of the American Society for Microbiology, Detroit, Mich., 5-10 May 1968. Taken from a dissertation submitted by the senior author in partial fulfillment of the requirements for the Ph.D. degree in Microbiology. measuring exchange reactions and analogue inhibition patterns. In the experiments reported, vitamin uptake was measured in nonproliferating yeast cells. MATERIALS AND METHODS Chemicals. Crystalline d-biotin was purchased from the Sigma Chemical Co., St. Louis, Mo. Carbonyl-labeled 14C-biotin (57.5 mc/mmole) was obtained from Amersham-Searle Co., Des Plaines, Ill. The purity of the labeled biotin was checked by bioand radioautography. dl-Desthiobiotin was purchased from Nutritional Biochemicals Corp., Cleveland, Ohio. The biotin analogues d-homobiotin, dl-oxybiotin, and d-norbiotin were gifts from Hoffmann

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“…Oxybiotin, an analogue of biotin in which the sulfur is replaced by an oxygen atom, has been shown to be as effective as biotin in promoting the growth of lactobacilli (5,8,10). Unlike other biotin analogues, oxybiotin functions without being converted to biotin (1).…”

Section: Resultsmentioning

confidence: 99%

“…We reported that Lactobacillus plantarum converts biotin sequentially to an avidin-combinable and -uncombinable vitamer, the latter being impermeable to the organism (1). Krueger and Peterson (8) have reported that a similar organism, L. pentosus, degrades biotin to products not utilizable by Saccharomyces cerevisiae, which suggests that no vitamers accumulated. They demonstrated, also, that oxybiotin undergoes the same degradative process.…”

mentioning

confidence: 99%

Metabolism of Biotin and Analogues of Biotin by Microorganisms III. Degradation of Oxybiotin and Desthiobiotin by Lactobacillus plantarum

Birnbaum

Lichstein

1966

Journal of Bacteriology

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, AND HERMAN C. LICHSTEIN. Metabolism of biotin and analogues of biotin by microorganisms. III. Degradation of oxybiotin and desthiobiotin by Lactobacillus plantarum. J. Bacteriol 92:920-924. 1966.-Lactobacillus plantarum growing in excess oxybiotin degraded a portion to products not utilizable by Saccharomyces cerevisiae. The loss of activity for the yeast suggested that no vitamers of oxybiotin accumulated during the degradation. The initiation of degrading activity was controlled by the pH of the growth medium and appeared during early stationary phase. Only cells grown in excess oxybiotin could degrade this biotin analogue. Nonproliferating cells grown previously in excess oxybiotin were able to convert biotin to vitamers (active for the yeast) as well as to degrade oxybiotin. Those grown in excess biotin also developed the ability to degrade oxybiotin as well as to convert biotin; however, in this case, the enzymes degenerated more rapidly. Cells grown with excessive amounts of either material were able to degrade desthiobiotin to products not available for the yeast. Both biotin conversion and oxybiotin degradation were found to have the same requirements for Mg and Mn ions. It was concluded that conversion of biotin to vitamers, and the degradation of oxybiotin or desthiobiotin are functions of the same on closely related enzyme systems.

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Metabolism of Biotin and Oxybiotin by Lactobacillus pentosus 124-2

Abstract: The demonstration of biotin activity for microorganisms of such analogs of biotin as oxybiotin (

Cited by 34 publications

References 21 publications

Studies on the Vitamin Nutrition of Allescheria Boydii Shear

Studies on the Vitamin Nutrition of Allescheria Boydii Shear

Characterization of the Biotin Transport System in Saccharomyces cerevisiae

Metabolism of Biotin and Analogues of Biotin by Microorganisms III. Degradation of Oxybiotin and Desthiobiotin by Lactobacillus plantarum

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