Metabolism of diethylstilbestrol in the C3H mouse: Chromatographic systems for the quantitative analysis of DES metabolic products

A method was developed t o i d e n t i f y r a d i o l a b e l e d DES and DES m e t a b o l i t e s i n b i o l o g i c a l f l u i d s and tissues. A f t e r a r a p i d i n i t i a l clean-up step, t h e b i o l o g i c a l samples were analyzed w i t h a single-column, reversed-phase, highpressure 1 i q u i d chromatography system. The parent compound and m e t a b o l i t e s were simultaneously resolved and t e n t a t i v e l y i d e n t i f i e d by comparing t h e i r r e t e n t i o n times t o those o f known standards. P o s i t i v e i d e n t i f i c a t i o n o f some m e t a b o l i t e s was achieved by f i e l d -d e s o r p t i o n and c a p i l lary-column massspectrometric analysis. and sample recovery was >80%. The method was found t o be r a p i d and r e p r o d u c i b l e ,

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Metabolism of diethylstilbestrol in the C3H mouse: Chromatographic systems for the quantitative analysis of DES metabolic products

Cited by 4 publications

References 25 publications

Progesterone Suppression of the Estradiol Receptor in the Reproductive Tract of Macaques, Cats, and Hamsters

Progesterone Suppression of the Estradiol Receptor in the Reproductive Tract of Macaques, Cats, and Hamsters

Diethylstilboestrol: II, pharmacology, toxicology and carcinogenicity in experimental animals

Separation of Diethylstilbestrol and Derivatives in Biological Fluids and Tissues by High Pressure Liquid Chromatography

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