Metabolism of Fenitrothion in Goats

Abstract

The lactating Japanese Sannen goats were treated orally with 14C-ring labeled fenitrothion [O, O-dimethyl O-(3-methyl-4-nitrophenyl)phosphorothioate, Sumithion(R)] at 0.5 mg/kg/day for 7 days. At 1 day after treatment, no residues of intact fenitrothion were found in the organs and tissues, and a little amount of aminofenitrothion was detected in digestive tracts (rumen, omasus and large intestine). The radiocarbon disappeared rapidly thereafter. The administered radiocarbon was essentially quantitatively excreted during a week post-treatment period; 50% of the dose were recovered in urine, 44% in feces, and 0.1 % in milk with a maximum of 0.011 ppm equivalent to the parent compound. The remaining radioactivity was further excreted, although gradually. The major metabolites in urine, feces and milk were O, O-dimethyl O-(3-methyl-4-aminophenyl)phosphorothioate (aminofenitrothion) and O-methyl-O-hydrogen O-(3-methyl-4-acetylaminophenyl)phosphate, and O, O-dimethyl O-(3-methyl-4-sulfo aminophenyl)phosphorothioate (Nsulfo aminofenitrothion), respectively. No intact fenitrothion nor fenitrooxon was found in milk, urine and feces.

Metabolism of an Insecticide Fenitrothion by Cunninghamella elegans ATCC36112

“…22,23,36 Cytochrome P450 was considered to be responsible for those metabolisms (phase I metabolism). 21,45 The two major degradation pathways of FNT by C. elegans in the current study were also found in other animals, such as rats, 46 female goats, 47 and birds. 48 Furthermore, aromatic hydroxylation during the metabolism of FNT in birds, 48 which has not reported in plants and soil, was also detected in C. elegans.…”

“…elegans can metabolize a wide variety of xenobiotics as those reported in human and animal studies through N-oxidation and N-deethylation, , demethylation, and hydroxylation. ,, Cytochrome P450 was considered to be responsible for those metabolisms (phase I metabolism). , The two major degradation pathways of FNT by C. elegans in the current study were also found in other animals, such as rats, female goats, and birds . Furthermore, aromatic hydroxylation during the metabolism of FNT in birds, which has not reported in plants and soil, was also detected in C.…”

Metabolism of Fenitrothion and Conjugation of 3-Methyl-4-nitrophenol in Tomato Plant (Lycopersicon esculentum)

“…) is an organophosphorus insecticide that has been used worldwide for forest protection and control of various plant and household insects (1,2). Although the various metabolic studies of 1 have been extensively studied in mammals (3), soil (4), and fish as well as abiotic degradation (4,5), its metabolic profiles in plants have not been fully clarified (6)(7)(8). In apple (Malus sp.)…”

“…The extract was applied to a Sep-pak cartridge and successively eluted with 10 mL of distilled water and 10 mL of a mixture of methanol and distilled water (2/8, v/v) and finally with 10 mL of methanol. The eluates from the mixture solvent were combined and concentrated and then subjected to the repeated purification using by HPLC (methods [3][4][5][6].…”

Metabolism of Fenitrothion in Birds

“…14C -Fenitrothion (5 .54 mCi/mmol, uniformly labeled in the phenyl ring) was prepared by Yoshitake et al13) of Institute for Biological Science, Sumitomo Chemical Co., Ltd. Radiochemical purity of the preparation as determined by radio-thin layer chromatography was above 99%. The following non-active authentic compounds were synthesized in this labora-tory4, 11,12); fenitrothion, fenitrooxon [0, 0dimethyl 0 - 3-methyl-4-nitrophenol, 3methyl-4-nitrophenyl sulfate, 3-hydroxymethyl-4-nitrophenol, 3-hydroxymethyl-4-nitrophenyl sulfate, 3-methyl-4-acetylaminophenol and 3-methyl-4-acetylaminophenyl sulfate. Calf liver /3-glucuronidase and limpet arylsulfatase type III were purchased from Sigma Chemical Co. (St. Louis, Mo., USA).…”