2014
DOI: 10.1016/j.bcp.2014.09.004
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Metabolism of gamma hydroxybutyrate in human hepatoma HepG2 cells by the aldo-keto reductase AKR1A1

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Cited by 13 publications
(9 citation statements)
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References 45 publications
(54 reference statements)
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“…63 Other results from experiments performed on multiple cell types (including human brain and hepatoma cell lines) support the transformation of accumulated GHB by a dehydrogenase identified as AKR1A1 using short interfering RNA. 64,65 This would lead to the synthesis of SSA, which may serve as precursor for GABA or may be the substrate for SSADH.…”
Section: The Endogenous Ghb System In Brainmentioning
confidence: 99%
“…63 Other results from experiments performed on multiple cell types (including human brain and hepatoma cell lines) support the transformation of accumulated GHB by a dehydrogenase identified as AKR1A1 using short interfering RNA. 64,65 This would lead to the synthesis of SSA, which may serve as precursor for GABA or may be the substrate for SSADH.…”
Section: The Endogenous Ghb System In Brainmentioning
confidence: 99%
“…It is a hepatoblastoma cell line derived from a 15-year-old male of European ancestry (4,5). Representing the human endodermal lineage, HepG2 cells are widely used as models for human toxicology studies (6–10), including toxicogenomic screens using CRISPR-Cas9 (11), in addition to studies on drug metabolism (12), cancer (13), liver disease (14), gene regulatory mechanisms (15) and biomarker discovery (16). As one of the main cell lines of the ENCyclopedia Of DNA Elements Project (ENCODE), HepG2 has been used to generate close to 1000 datasets for ENCODE (17).…”
Section: Introductionmentioning
confidence: 99%
“…Expression of these enzymes has been demonstrated in the brain, liver, and kidney (5052). Enzyme kinetic estimates for AKR1A1-mediated GHB metabolism have been evaluated in hepatocytes and suggest that AKR1A1 has a low affinity and high capacity for GHB metabolism (53). Partitioning into metabolic tissues must be corrected for the intrinsic clearance of a drug within the tissue.…”
Section: Discussionmentioning
confidence: 99%