Metabolism of Glycerol by an Acrolein-Forming Lacto-Bacillus

Sobolov, Murray; Smiley, K. L.

doi:10.1128/jb.79.2.261-266.1960

Cited by 84 publications

(47 citation statements)

References 14 publications

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“…Few micro-organisms have the ability to use glycerol in a reductive branch. In this degradation pathway, it is converted in a two-step process to 1,3-propanediol (1,3-PD) excreted into the extracellular medium [1]. The ¢rst reaction involves the transformation of glycerol into 3-hydroxypropionaldehyde (3-HPA) by a coenzyme B-12-dependent dehydratase enzyme.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the diol dehydratase pdu operon of Lactobacillus collinoides

Sauvageot¹

2002

FEMS Microbiology Letters

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The three genes (pduCDE) encoding the diol dehydratase of Lactobacillus collinoides were sequenced. They exhibited strong identities with the ddrABC and pduCDE genes of Klebsiella oxytoca and Salmonella enterica, respectively. These genes are part of a putative operon with at least four other genes. An eighth open reading frame was identified as homologous to the pocR gene (encoding the operon regulatory protein). Although the enzyme was detected in exponential growth phase, PduCDE activity was increased at the end of exponential phase in presence of 1,2‐propanediol.

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Section: Introductionmentioning

confidence: 99%

Characterisation of the diol dehydratase pdu operon of Lactobacillus collinoides

Sauvageot¹

2002

FEMS Microbiology Letters

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“…Lactobacillus collinoides, recently isolated from cider, degraded glycerol in the presence of sugar mainly to PDO, acetic acid and ethanol [39], whereas Pediococcus pentosaceus, which was isolated from beer, mainly produces lactic acid and 2,3-butanediol as by-products [40]. Lactobacillus reuteri [41] and related strains have attracted attention since they are able to produce an antimicrobial agent called reuterin, containing 3hydroxypropionaldehyde (3HPA), which is further degraded to the toxic acrolein [42]. There is also extensive research on the biotechnological production of 3HPA and acrolein, both promising intermediates for the chemical industry [43].…”

Section: Lactobacillimentioning

confidence: 99%

Biotransformation of glycerol into 1,3‐propanediol

Willke

Vorlop

2008

Euro J Lipid Sci & Tech

104

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Today, glycerol is mainly a by-product of fat splitting and biodiesel production. Further growth of the biodiesel market would result in a fall in the price of glycerol. Particularly glycerol-water from rapeseed oil methyl ester production, for example, would be an interesting raw material if it could be utilised in fermentation without further pretreatment. Under anaerobic conditions, bacteria can transform glycerol into 1,3-propanediol (PDO), which can be used as a monomer in the chemical industry. PDO can be produced biotechnologically from glycerol with the aid of bacteria. Another way would be the utilization of glucose instead of glycerol, which would provide independence from the fluctuating glycerol market. However, under certain conditions, the classic technique based on glycerol can be quite interesting with regard to technical and economic aspects: A concerted, extensive search for new microorganisms (screening) and improved process design (fed-batch with pH-controlled substrate dosage) allowed the product concentrations, which were relatively low at a maximum of 70-80 g/L as a result of product inhibition, to be raised to more than 100 g/L. An additional advantage of the new technique and the newly isolated strains is the utilisation of low-priced crude glycerol or glycerol-water. This is a factor which should not be underestimated and has a direct effect on the product costs. Further on, the use of immobilised cells compared to freely suspended cells enables an increase of productivity from about 2 up to 30 g /Lh.

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“…3 Furthermore, microbes of the Lactobacillus, Klebsiella and Clostridium genus have been reported to convert glycerol to mainly 1,3-PDO, butanol, ethanol, butyric acid, acetate and others. [4][5][6][7][8][9] Whereas substrate inhibition during pure glycerol fermentation is observed only in very high concentrations, cell proliferation in moderate or even low crude glycerol concentrations is challenging. [10][11][12] The latter can be attributed to impurities that are found in the crude glycerol solution and can hinder bacterial growth.…”

Section: Introductionmentioning

confidence: 99%

Determining butanol inhibition kinetics on the growth of Clostridium pasteurianum based on continuous operation and pulse substrate additions

Kalafatakis

Skiadas

Gavala

2019

J of Chemical Tech & Biotech

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BACKGROUND Clostridium pasteurianum is a well described strain for the conversion of glycerol into butanol. In general, cell growth kinetics depends on the type of glycerol used and the concentration of butanol in the fermentation broth. However, despite the numerous studies existing on the subject, there is limited information in the literature regarding growth inhibition kinetics due to the cytotoxic effect of butanol on cell growth. This can be attributed to the difficulty of growing cells at high butanol concentration which renders the determination of inhibition kinetics a rather challenging task. RESULTS During this study a new approach for the determination of butanol inhibition kinetics on the growth of C. pasteurianum was tested. Specifically, pulses of crude glycerol were applied when steady state was reached during continuous fermentation experiments with increasing butanol concentration in the feed. Combining pulse experiments with batch fermentation at low substrate concentration allowed for accurate determination of the kinetic constants for inhibited growth. This approach also minimized the correlation of the growth constants which often leads to poor identifiability. CONCLUSION The proposed experimental approach showed good identifiability of the kinetic parameters for butanol inhibition of the microbial growth and can be proven valuable for the determination of inhibitory effects of highly toxic compounds. © 2019 Society of Chemical Industry

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Metabolism of Glycerol by an Acrolein-Forming Lacto-Bacillus

Cited by 84 publications

References 14 publications

Characterisation of the diol dehydratase pdu operon of Lactobacillus collinoides

Characterisation of the diol dehydratase pdu operon of Lactobacillus collinoides

Biotransformation of glycerol into 1,3‐propanediol

Determining butanol inhibition kinetics on the growth of Clostridium pasteurianum based on continuous operation and pulse substrate additions

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