Metabolism of MDCK cells during cell growth and influenza virus production in large-scale microcarrier culture

High yields of influenza A virus in Madin-Darby canine kidney cells are promoted by an insufficient interferon-induced antiviral state

“…Experiments with adherent MDCK cells (ECACC 84121903) and virus infections were performed in six-or 24-well plates. Culture and infection conditions were as described previously (Genzel et al, 2004). Stocks of influenza viruses PR8-NIBSC, PR8-RKI and WSN33 (a gift from Georg Kochs, University of Freiberg, Germany) were prepared in MDCK cells.…”

Downstream processing of MDCK cell-derived equine influenza virus

“…MDCK cells were grown in roller bottles (Greiner, Esslingen, Germany, 850 cm 2 ) containing 250 mL cell growth medium (Sigma-Aldrich, Germany) for inoculum preparation. The detailed procedure is described elsewhere [17].…”

Overcoming nutrient limitations for cell-based production of influenza vaccine

“…To address this issue, , current strategies include both simple and straightforward processes of fresh medium addition 12 and medium exchanging, and the relatively complex processes like fedbatch and perfusion. [13][14][15][16][17][18][19] In particular, one reported approach utilizes an alternating tangential flow (ATF) system. 18 With eight successful high cell-density ATF perfusion runs for the production of H1N1 virus, it was demonstrated that cell-specific virus yields could be kept constant and cell-specific infectious virus titers were even higher than those of batch cultures (Fig.…”