Metabolism of non-growing bacteria

Lempp, Martin; Lubrano, Paul; Bange, Gert; Link, Hannes

doi:10.1515/hsz-2020-0201

Cited by 40 publications

(39 citation statements)

References 44 publications

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“…Meanwhile, it showed higher activity against the Gram-negative P. aeruginosa compared to Gram-positive MRSA ( Figure 2 C). These different reactions may be ascribed to the differences of microbial membrane structure and component between the microbial strains [ 32 , 33 ]. The negative control group (co-solvent) had no inhibitory effect on the tested strains.…”

Section: Resultsmentioning

confidence: 99%

UPLC-ESI-MS/MS Based Characterization of Active Flavonoids from Apocynum spp. and Anti-Bacteria Assay

Gao

Ning

et al. 2021

Antioxidants

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In the current study, the active flavonoids from Apocynum venetum and Apocynum hendersonii leaf were efficiently characterized using UPLC-ESI-MS/MS, and yielding the highest content of 15.35 mg/g (A. venetum) and 13.28 mg/g (A. hendersonii) respectively. The antioxidant assay in vitro showed that the isolated flavonoid ingredient groups exhibited free radical scavenging activities to DPPH, ABTS and linoleic acid. The antimicrobial assay revealed the isolated flavonoid ingredient from both A. venetum and A. hendorsonii have exerted anti-MRSA and anti-P. aeruginosa effect through disrupting cell integrity and declining ATP. In vivo assay demonstrated that these flavonoid ingredients effectively accelerated MRSA-infected and P. aeruginosa-infected Balb/c mice wound healing. In summary, these results showed that the characterized flavonoid ingredients exhibited great potential as natural antioxidant and antimicrobial agents, and shed light into future potential applications of Apocynum spp.

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Section: Resultsmentioning

confidence: 99%

UPLC-ESI-MS/MS Based Characterization of Active Flavonoids from Apocynum spp. and Anti-Bacteria Assay

Gao

Ning

et al. 2021

Antioxidants

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“…Environmental bacteria do not divide actively in the natural niches they colonize, yet the cells are exposed to stressful conditions under these near-zero growth state—as mimicked in our current experimental design. Moreover, exploring nongrowing conditions has a significant impact on the design of industrial biocatalysts and bioprocesses [ 64 ], where stressful conditions are prevalent—and P . putida KT2440 is naturally primed to endow such circumstances [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

et al. 2021

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As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous oxidative stress. Yet, the ultimate reason of such phenotypic property remains largely unknown. To shed light on this question, metabolic network-wide routes for NADPH generation—the metabolic currency that fuels redox-stress quenching mechanisms—were inspected when P. putida KT2440 was challenged with a sub-lethal H2O2 dose as a proxy of oxidative conditions. 13C-tracer experiments, metabolomics, and flux analysis, together with the assessment of physiological parameters and measurement of enzymatic activities, revealed a substantial flux reconfiguration in oxidative environments. In particular, periplasmic glucose processing was rerouted to cytoplasmic oxidation, and the cyclic operation of the pentose phosphate pathway led to significant NADPH-forming fluxes, exceeding biosynthetic demands by ~50%. The resulting NADPH surplus, in turn, fueled the glutathione system for H2O2 reduction. These properties not only account for the tolerance of P. putida to environmental insults—some of which end up in the formation of reactive oxygen species—but they also highlight the value of this bacterial host as a platform for environmental bioremediation and metabolic engineering.

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“…( Mouton et al., 2018 ). However, MIC’s reliance on growth inhibition can be time-consuming (frequently exceeding 24 h for common pathogens ( Berkow et al., 2020 ), and also results in the inability to detect NGMA cells of C. albicans whose metabolic activity may cause reinfections after clinical latency ( Manina and McKinney, 2013 ; Manina et al., 2015 ; Lempp et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Assessing Efficacy of Clinical Disinfectants for Pathogenic Fungi by Single-Cell Raman Microspectroscopy

Фан

Ren

Chen

et al. 2022

Front. Cell. Infect. Microbiol.

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Disinfectants are crucial for root canal therapy (RCT), as metabolism of canal-inhabiting microbes can cause refractory infections. To develop effective yet patient- and environment-friendly disinfectant formulations, we quantitatively assessed the metabolism-inhibiting effects of intracanal disinfectants via D2O-probed Single-Cell Raman Spectra (SCRS), using Candida albicans (C. albicans) as a pathogen model. For chlorhexidine gluconate (CHX), sodium hypochlorite (NaClO), and hydrogen peroxide (H2O2), at their MIC of 4, 168, and 60 μg/ml, respectively, despite the complete growth halt, metabolic activity of individual fungal cells was reduced on average by 0.4%, 93.9%, and 94.1% at 8 h, revealing a “nongrowing but metabolically active” (NGMA) state that may underlie potential refractory infections, particularly for CHX. In contrast, at their Metabolic Activity-based Minimum Inhibitory Concentrations (MIC-MA) of 8, 336, and 120 μg/ml, respectively, metabolic activity of all cells was completely halted throughout 8 h exposure. Moreover, combined use of NaClO+H2O2 (combination at 0.5× MIC-MA each) outperforms solo uses of CHX, NaClO, H2O2, or other binary combinations. Furthermore, dynamics of SCRS revealed distinct fungicidal mechanisms of CHX, NaClO, H2O2, and their pairwise combinations. MIC-MA is advantageous in critically assessing antifungal efficacy, and NaClO+H2O2 can potentially serve as a more efficient disinfectant formula for fungal pathogens.

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Metabolism of non-growing bacteria

Cited by 40 publications

References 44 publications

UPLC-ESI-MS/MS Based Characterization of Active Flavonoids from Apocynum spp. and Anti-Bacteria Assay

UPLC-ESI-MS/MS Based Characterization of Active Flavonoids from Apocynum spp. and Anti-Bacteria Assay

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

Assessing Efficacy of Clinical Disinfectants for Pathogenic Fungi by Single-Cell Raman Microspectroscopy

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