Metabolism of S-Benzyl O, O-Diisopropyl Phosphorothioate (Kitazin P®) by Mycelial Cells of Pyricularia oryzae

Tomizawa, Chōjirō; Uesugi, Yasuhiko

doi:10.1271/bbb1961.36.294

Cited by 19 publications

(6 citation statements)

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“…Metabolism of PTL fungicides by wildtype and PTL-resistant strains of P. oryzae has been investigated and similar results have been reported Tomizawa, 1971, 1972;Tomizawa and Uesugi, 1972). IBP metabolism involves detoxification because its main metabolite, O,O-diisopropyl hydrogen phosphorothioate, is by no means fungicidal.…”

Section: Discussionsupporting

confidence: 54%

Back-mutation of an organophosphorus thiolate-resistant mutant of Pyricularia oryzae

Uesugi

Katagiri

1977

Neth. J. Pl. Path.

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From a large number of conidia of a mutant of Pyricularia oryzae resistant to organophosphorus thiolate (PTL) fungicides (mutant I), a new mutant was selected on agar plates containing a phosphoramidate (PA). The latter compound was found to be more fungicidal to mutant I than to the wild-type strain. The PA-resistant mutant obtained by this selection (mutant II) was as sensitive as the wild-type strain to PTL fungicides. A synergistic joint action of PTL and PA, as shown against the wild-type strain but not against mutant I, was also shown against mutant II. The wild-type strain and mutant I and II were compared regarding their metabolism of PTL and PA compounds. Marked differences were hardly found among the three strains when they were incubated with low dosages of either PTL or PA. At higher dosages of either PTL or PA which affected the three strains differentially, the wild-type strain as well as mutant II metabolized PTL and PA far more rapidly than mutant I did. Therefore, in the present experiments mutant II was similar to the wild-type strain with regard to sensitivity to PTL and PA and to their joint action, and with respect to ability to metabolize these compounds.

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Section: Discussionsupporting

confidence: 54%

Back-mutation of an organophosphorus thiolate-resistant mutant of Pyricularia oryzae

Uesugi

Katagiri

1977

Neth. J. Pl. Path.

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“…Strains ofP. oryzae with a high degree of resistance tO IBP metabolize the fungicide at a normal rate (Tomizawa and Uesugi, 1972). In that case the tentative explanation for resistance to IBP, coupled with collateral sensitivity to BPA as given by Uesugi and Sisler (1978) might apply.…”

Section: Discussionmentioning

confidence: 99%

“…Inability to break down EBP or edifenphos would enhance their fungitoxicity because metabolites of these fungicides are not fungitoxic (Uesugi and Tomizawa, 1971 ;Tomizawa and Uesugi, 1972). This does not hold since no negative crossresistance with pyrazophos was found.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of resistance to pyrazophos in Pyricularia oryzae

Waard

Nistelrooy

1980

Netherlands Journal of Plant Pathology

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Pyrazophos-resistant strains of Pyricularia oryzae were isolated from sectors in colonies of the fungus on a pyrazophos-containing agar medium. In contrast to the wild-type strain, resistant strains did not metabolize pyrazophos (Afugan, Curamil) into its phosphate analogue and 2-hydroxy-5-methyl-6-ethoxycarbonylpyrazolo(l,5-a)pyrimidine (PP), which is regarded as the fungitoxic principle of pyrazophos. Resistant strains possessed a slightly increased sensitivity to PP. These data indicate that the mechanism of resistance to pyrazophos is not related to a change of the target sites of PP, but rather with inability to convert pyrazophos into PP. The pyrazophos-resistant strains displayed cross-resistance to the organophosphorus fungicides EBP (Kitazin) and edifenphos (Hinosan). The mechanism of cross-resistance involved is not understood.

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“…The role of non-oxidative metabolism of organophosphates is largely unknown, except for the reductive reactions that yield such products as aminoparathion. Oxidative desulfuration of a thionate in procaryotes has never been shown, although the absence of the oxon, during metabolism of a thionate, has been noted in nearly all investigations of bacterial (2,5,41,43,44) and fungal (41,67) transformations of phosphorothionates.…”

Section: Discussionmentioning

confidence: 99%

Parathion utilization by bacterial symbionts in a chemostat

Daughton¹,

Hsieh²

1977

Appl Environ Microbiol

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A continuous-culture device was used to select and enrich for microorganisms, from sewage and agricultural runoff, that were capable of using the organophosphorus insecticide parathion as a sole growth substrate. Parathion was dissimilated by the highly acclimated symbiotic activities of Pseudomonas stutzeri, which non-oxidatively and cometabolically hydrolyzed the parathion to ionic diethyl thiophosphate and p-nitrophenol, and P. aeruginosa, which utilized the p-nitrophenol as a sole carbon and energy source. Ionic diethyl thiophosphate was found to be inert to any transformations. Methyl parathion was dissimilated in an analogous way. The device functioned as a chemostat with parathion as the growth-limiting nutrient, and extraordinarily high dissimilation rates were attained for parathion (8 g/liter per day) and for p-nitrophenol (7 g/liter per day). This is the first report of parathion utilization by a defined microbial culture and by symbiotic microbial attack and of dissimilation of an organophosphorus pesticide in a chemostat. Continuous-cultivation techniques have rarely been used for studying the interactions of microorganisms and xenobiotics, e.g., microbial degradation of pesticides and toxic wastes.

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Metabolism of S-Benzyl O, O-Diisopropyl Phosphorothioate (Kitazin P®) by Mycelial Cells of Pyricularia oryzae

Cited by 19 publications

References 0 publications

Back-mutation of an organophosphorus thiolate-resistant mutant of Pyricularia oryzae

Back-mutation of an organophosphorus thiolate-resistant mutant of Pyricularia oryzae

Mechanism of resistance to pyrazophos in Pyricularia oryzae

Parathion utilization by bacterial symbionts in a chemostat

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