Metabolism of steroids in plant tissue cultures

Stohs, Sidney J.

doi:10.1007/3540098070_9

Cited by 5 publications

(6 citation statements)

References 68 publications

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“…Selective transformations in high yields have been the basis of the numerous applications (82,93,94). Both microbial (95,96) and plant sources (97,98) have been used as sources of the specific biocatalysts for particular reactions. Special emphasis presently is being placed on the application of immobilized systems (94)(95)(96)98,99).…”

Section: Steroid Transformations the Pharmaceutical Industrymentioning

confidence: 99%

“…Major attention has been paid to biocatalysts for specific reactions involving sterol side-chain cleavage, reductions, hydroxylations, isomerizations, etc. (94)(95)(96)(97). Thus, for example, cholesterol can be converted to 20-carboxy-pregna-l,3-dien-3-one, in 95% yield, by a mutant of Corynebacterium sp.…”

Section: Steroid Transformations the Pharmaceutical Industrymentioning

confidence: 99%

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Biotechnology and the fats and oils industry — An overview

Rattray

1984

J Americ Oil Chem Soc

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Biotechnology is the application of single or multicellular organisms and of associated or derived enzyme systems to the production of desirable products. Particular discussion has been made of the derivation of fats and oils from animals, plants and microorganisms. General consideration has been given to methods, primarily plant breeding and agronomic practices for the improvement of the quantity and quality of oil produced by soybean, rapeseed, palm and sunflower. The possible importance of yeasts, fungi and algae as sources of single cell oil has been examined. A particular role of these systems in the production of specialty oils has been suggested. Enzyme systems, either associated with the intact cell or in isolation, can be used to varying degrees of success in either a free or immobilized form. Particular reference has been made to application of these systems to reactions including specific hydrolysis of triacyb glycerols, acylation of glycerol, interesterification of triacylglycerols, wax ester formation and steroid transformations. Consideration has been given to particular plants and microorganisms as sources of new fats and oils. The major impact of biotechnology on the industry is believed to be associated with the production of high value specialty products including cocoa butter substitutes, biosurfactants, waxes and various prostaglandin derivatives. General consideration has been given to the possible relative importance of plant and microbial systems, engineering and scale-up problems, and overall economics of present biotechnological procedures.

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Section: Steroid Transformations the Pharmaceutical Industrymentioning

confidence: 99%

Section: Steroid Transformations the Pharmaceutical Industrymentioning

confidence: 99%

Biotechnology and the fats and oils industry — An overview

Rattray

1984

J Americ Oil Chem Soc

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“…EHRHART, et al [28]; HEITMAN [29]; STOHS and ROSENBERG [30]; OVERTON [31]; STOHS [32,33]; and LARGEAU, et al [34].…”

Section: Partial Metabolic Pathwaymentioning

confidence: 99%

“…It is promising that the dominant sterols are obtained in higher quantities from the sterile callus tissue culture cells than from the whole plant; hence, selecting cell lines for high yields of sterols may permit producing a sterol of choice in quantity. Stereospecific bioconversions of some common cheaper precursors might be utilized to produce desired sterols [33,35], which could be isolated readily. Thus, tissue culture production shows promise in providing drugs and chemicals now made by slow, costly extraction from whole plant parts [33,[35][36][37].…”

Section: Partial Metabolic Pathwaymentioning

confidence: 99%

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Sterols ofDelphinium ajacis; Production and Metabolic Relationships in Whole Plants and Callus Tissue*

Waller¹,

Mangiafico²,

Foster³

et al. 1981

Planta Med

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Sterols from whole nonsterile Delphinium ajacis plants and from sterile tissue cultures (callus) were identified and determined quantitatively. The major sterols in the whole plant tissues were sitosterol, campesterol and stigmasterol, whereas those in the callus tissue were stigmastanol, 24-ethylidenelophenol and Delta (7)-stigmastanol. Of the 21 compounds identified in callus tissue, 5 were not present in the whole plant, most notably Delta (7)-stigmastanol. For both sources of tissue, the sterol predominating in one was a minor component in the other (whole plant/tissue cultures: sitosterol 57%/5%; stigmastanol 2%/35%). On a tissue dry-weight basis, the amount of sterols isolated from callus tissue culture was ten to twenty times that obtained from the whole plant. Qualitatively the sterols from both sources fit into a metabolic scheme which proceeds from cycloartenol through 4,4-dimethylsterols and 4-methylsterols to sterols. A proposed metabolic pathway shows the differences in accumulation of sterols in the two types of tissue. The increase in sterol production in cultured cells, especially when favored by growth conditions, has promise for industrial application and in organic synthesis.

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Lipids

Weber¹,

Mangold²

1988

Phytochemicals in Plant Cell Cultures

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Metabolism of steroids in plant tissue cultures

Cited by 5 publications

References 68 publications

Biotechnology and the fats and oils industry — An overview

Biotechnology and the fats and oils industry — An overview

Sterols ofDelphinium ajacis; Production and Metabolic Relationships in Whole Plants and Callus Tissue*

Lipids

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