Metabolism of Urea Nitrogen by Mixed Cultures of Rumen Bacteria Grown on Cellulose

Winter, K. A.; Johnson, R. R.; Dehority, Burk A.

doi:10.3168/jds.s0022-0302(64)88766-x

Cited by 57 publications

(28 citation statements)

References 13 publications

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“…Protein nitrogen in unstrained omasal digesta was determined by the method of Winter, Johnson, and Dehority (1964), except that, initially, equal volumes (10 mI) of the tungstate and sulphuric acid solutions were added to 10 g of sample.…”

Section: (D) Chemical Analybi8mentioning

confidence: 99%

Sulphur Metabolism and Excretion Studies in Ruminants IX. Sulphur, Nitrogen, and Energy Utilization by Sheep Fed a Sulphur-Deficient and a Sulphate-Supplemented, Roughage-Based Diet

Bird¹

1972

Aust. Jnl. Of Bio. Sci.

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Four Merino wethers were each fed a sulphur-deficient, roughage-based ration containing 1'05% nitrogen (79% as urea) and supplying 135 mg sulphur/day. Four other sheep were fed similar amounts of basal ration supplemented with NasS04. This ration supplied 494 mg sulphur/day. Mter 21-day periods the treatment groups were reversed.Supplemental sulphate increased the daily flow of protein nitrogen to the omasum by 2·09 (±0·28) g/day (mean ± S.E.M.) (p<0·001) and changed the nitrogen balance from -2·38 (±0·09) g/day to 0·15 (±0·19) g/day (P

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Section: (D) Chemical Analybi8mentioning

confidence: 99%

Sulphur Metabolism and Excretion Studies in Ruminants IX. Sulphur, Nitrogen, and Energy Utilization by Sheep Fed a Sulphur-Deficient and a Sulphate-Supplemented, Roughage-Based Diet

Bird¹

1972

Aust. Jnl. Of Bio. Sci.

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“…Samples were centrifuged at 15 000 g for 15 min, and the supernatant was analyzed by spectrophotometry (Libra S21, Biochrom Technology, Cambridge, UK) for ammonia-N (Chaney and Marbach 1962). Results were used to calculate large peptides (LPep = TCA-N−TA-N) and small peptides (SPep) plus amino acids (SPep = TA-N−ammonia-N) as proposed by Winter et al (1964). Samples for lactic acid analysis were prepared as described by Jouany (1982) and analyzed by gas chromatography: 1 mL of a solution made up of a 2 g L −1 solution of mercuric chloride, 2 g L −1 of 4-methylvaleric acid as an internal standard, and 20 g L −1 orthophosphoric acid were added to 4 mL of silage juice and frozen.…”

Section: Sampling Chemical and Microbial Analysesmentioning

confidence: 99%

Effects of essential oil compounds addition on ryegrass silage protein degradation

et al. 2016

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“…Ammonia N concentrations were measured according to the method described by Rhine et al (1998). The tungstic acid and trichloroaceitic acid soluble N were determined as described by Winter et al (1964). Results were used to calculate (mg 100 mL…”

Section: Measurements In Rusitecmentioning

confidence: 99%

Effect of sainfoin hay and pomegranate peel extracts on in vitro fermentation and protein degradation using the RUSITEC technique

Refat

AneleU.

et al. 2015

Can. J. Anim. Sci.

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Effect of sainfoin hay and pomegranate peel extracts on in vitro fermentation and protein degradation using the RUSITEC technique. Can. J. Anim. Sci. 95: 417Á423. The objectives of this study were to determine the effect of supplementation of sainfoin hay extract (SHE) and pomegranate peel extract (PPE) on in vitro rumen fermentation characteristics, protein metabolism and nutrient digestibility using rumen simulation technique (RUSITEC). The experiment was a completely randomized design with three treatments and four replications. Dietary treatments were: (1) control (10% barley silage and 90% concentrate), and control supplemented (2) with SHE (4.1 g catechin equivalent kg(1 dry matter; DM) or (3) with PPE (2.8 g tannic acid equivalents kg(1 DM). The experiment consisted of 10 d of adaptation and 7 d of data collection. Concentration of total volatile fatty acid (VFA) was lower (PB0.02), but molar proportion of acetate was higher (PB0.01) with SHE or PPE than control. Branched-chain VFA decreased (PB0.01) with PPE supplementation compared with control. Concentrations of large and small peptides were not affected but ammonia nitrogen (NH 3 -N) concentration decreased (P B0.01) with PPE supplementation. Digestibility of DM, starch and crude protein decreased (PB0.02) with SHE and PPE supplementation compared with control. Bacterial nitrogen production (mg d(1 ) and bacterial efficiency (g bacterial nitrogen kg(1 digested organic matter) were not affected by SHE or PPE supplementation. Overall, there were no differences in VFA concentrations, nitrogen fractions and nutrient digestibility between SHE and PPE except for the concentrations of branched-chain VFA and NH 3 -N, which were higher (PB0.01) with SHE versus PPE supplementation. In conclusion, supplementation of high-grain diet with SHE or PPE can decrease rumen by-pass protein; however, suppression of feed fermentation may decrease the feeding value of high-grain diet.

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Metabolism of Urea Nitrogen by Mixed Cultures of Rumen Bacteria Grown on Cellulose

Cited by 57 publications

References 13 publications

Sulphur Metabolism and Excretion Studies in Ruminants IX. Sulphur, Nitrogen, and Energy Utilization by Sheep Fed a Sulphur-Deficient and a Sulphate-Supplemented, Roughage-Based Diet

Sulphur Metabolism and Excretion Studies in Ruminants IX. Sulphur, Nitrogen, and Energy Utilization by Sheep Fed a Sulphur-Deficient and a Sulphate-Supplemented, Roughage-Based Diet

Effects of essential oil compounds addition on ryegrass silage protein degradation

Effect of sainfoin hay and pomegranate peel extracts on in vitro fermentation and protein degradation using the RUSITEC technique

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