Metabolism ofD- andL-[13C]alanine in rat liver detected by1H and13C NMR spectroscopyin vivo andin vitro

Dölle, Andreas

doi:10.1002/(sici)1099-1492(200004)13:2<72::aid-nbm610>3.0.co;2-7

Cited by 8 publications

(3 citation statements)

References 45 publications

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“…In the last few decades magnetic resonance (MR) techniques have greatly expanded our understanding of metabolism and metabolic flux [1–4]. A disadvantage of MR, however, has been its lack of sensitivity compared with other imaging modalities like optical, positron emission tomography (PET), and single-photon emission computed tomography (SPECT).…”

Section: Introductionmentioning

confidence: 99%

Nuclear spin hyperpolarization of the solvent using signal amplification by reversible exchange (SABRE)

Moreno

Nasr

Milne

et al. 2015

Journal of Magnetic Resonance

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Here we report the polarization of the solvent OH protons by SABRE using standard iridium-based catalysts under slightly acidic conditions. Solvent polarization was observed in the presence of a variety of structurally similar N-donor substrates while no solvent enhancement was observed in the absence of substrate or para-hydrogen (p-H2). Solvent polarization was sensitive to the polarizing field and catalyst:substrate ratio in a manner similar to that of substrate protons. SABRE experiments with pyridine-d5 suggest a mechanism where hyperpolarization is transferred from the free substrate to the solvent by chemical exchange while measured hyperpolaization decay times suggest a complimentary mechanism which occurs by direct coordination of the solvent to the catalytic complex. We found the solvent hyperpolarization to decay nearly 3 times more slowly than its characteristic spin-lattice relaxation time suggesting that the hyperpolarized state of the solvent may be sufficiently long lived (~20 s) to hyperpolarize biomolecules having exchangeable protons. This route may offer future opportunities for SABRE to impact metabolic imaging.

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Section: Introductionmentioning

confidence: 99%

Nuclear spin hyperpolarization of the solvent using signal amplification by reversible exchange (SABRE)

Moreno

Nasr

Milne

et al. 2015

Journal of Magnetic Resonance

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“…This reaction together with the reversed transformation of alanine to pyruvate is catalyzed by the alanine transaminase also known as alanine aminotransferase (Dolle, 2000; Yang et al, 2009). The activity of this enzyme combined with the protein degradation and membrane decomposition, together with the presence of some free alanine inside the cells give likely explanation for the moderate increase in the alanine signal in the spectra of rat liver homogenates during their long-term observation.…”

Section: Resultsmentioning

confidence: 99%

Looking at microbial metabolism by high-resolution²H-NMR spectroscopy

Kutyshenko

Beskaravayny

Молчанов

et al. 2013

PeerJ

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We analyzed the applicability of high-resolution 2H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D2O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.

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“…Similar concepts may be applied to the physiological pathways regulating the higher preferences for L-alanine and L-serine over their D-isomers. 1 H and 13 C NMR spectroscopic studies upon infusion of L- and D-alanine labeled with 13 C in fasted rats show that D-alanine is metabolized significantly more slowly than the correspondingly labeled L-enantiomer (Dolle 2000). In addition, the immediate product of D-serine metabolism is likely to be hydroxypyruvate, rather than pyruvate, since hydroxypyruvate was identified as a metabolite of D,L-serine, but not of L-serine, in rat liver (Sprinson & Chargaff 1946; Nadkarni et al 1960).…”

Section: Discussionmentioning

confidence: 99%

Enantiomer-specific selection of amino acids

2013

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Dietary intake of L-amino acids impacts on several physiological functions, including the control of gastrointestinal motility, pancreatic secretion, and appetite. However, the biological mechanisms regulating behavioral predilections for certain amino acid types remain poorly understood. We tested the hypothesis that, in mice, the potency with which a given glucogenic amino acid increases glucose utilization reflects its rewarding properties. We have found that: 1. During long-, but not short-, term preference tests, L-alanine and L-serine were preferred over their D-enantiomer counterparts, while no such effect was observed for L-threonine vs. D-threonine; 2. These behavioral patterns were closely associated with the ability of L-amino acids to promote increases in respiratory exchange ratios such that those, and only those, L-amino acids able to promote increases in respiratory exchange ratios were preferred over their D-isomers; 3. These behavioral preferences were independent of gustatory influences, since taste-deficient Trpm5 knockout mice displayed ingestive responses very similar to those of their wild-type counterparts. We conclude that the ability to promote increases in respiratory exchange ratios enhances the reward value of nutritionally relevant amino acids, and suggest a mechanistic link between substrate utilization and amino acid preferences.

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Metabolism ofD- andL-[13C]alanine in rat liver detected by1H and13C NMR spectroscopyin vivo andin vitro

Cited by 8 publications

References 45 publications

Nuclear spin hyperpolarization of the solvent using signal amplification by reversible exchange (SABRE)

Nuclear spin hyperpolarization of the solvent using signal amplification by reversible exchange (SABRE)

Looking at microbial metabolism by high-resolution²H-NMR spectroscopy

Enantiomer-specific selection of amino acids

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Metabolism ofD- andL-[13C]alanine in rat liver detected by1H and13C NMR spectroscopyin vivo andin vitro

Cited by 8 publications

References 45 publications

Nuclear spin hyperpolarization of the solvent using signal amplification by reversible exchange (SABRE)

Nuclear spin hyperpolarization of the solvent using signal amplification by reversible exchange (SABRE)

Looking at microbial metabolism by high-resolution2H-NMR spectroscopy

Enantiomer-specific selection of amino acids

Contact Info

Product

Resources

About

Looking at microbial metabolism by high-resolution²H-NMR spectroscopy