Metabolism ofL-sorbose by enzymes fromGluconobacter melanogenus IFO 3293

Kitamura, Iwao; Perlman, D.

doi:10.1007/bf01385440

Cited by 8 publications

(2 citation statements)

References 9 publications

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“…Kitamura et al 2 ) reported that L-sorbose and L-sorbosone dehydrogenases of Gluconobacter melanogenus IFO 3293 were mainly located in the membrane fraction. In our ob- servation, L-sorbose dehydrogenase is located in the membrane fraction, but L-sorbosone dehydrogenase is mainly located in the cytosol fraction.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Pathway for 2-Keto-l-gulonic Acid Formation inGluconohacter melanogenusIFO 3293

Hoshino¹,

Sugisawa²,

Tazoe³

et al. 1990

Agricultural and Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

Metabolic Pathway for 2-Keto-l-gulonic Acid Formation inGluconohacter melanogenusIFO 3293

Hoshino¹,

Sugisawa²,

Tazoe³

et al. 1990

Agricultural and Biological Chemistry

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“…2-KGA is the direct precursor of L-ascorbic acid. It can be produced by oxidation of D-sorbitol or L-sorbose via Lsorbosone (Kitamura and Perlman, 1975a;1975b), or directly from glucose by oxidation at carbon 5.…”

Section: Simplification Of the Reichstein Proceduresmentioning

confidence: 99%

The GenusGluconobacterand Its Applications in Biotechnology

Macauley

McNeil

Harvey

2001

Critical Reviews in Biotechnology

106

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Organisms of the genus Gluconobacter have been widely utilized within the biotechnology industry for many decades, due to their unique metabolic characteristics. The metabolic features that render Gluconobacter so useful in biotransformation processes, vitamin synthesis, and, as the biological element in sensor systems, are critically evaluated, and the relevance of recent biochemical genetic studies to current and future industrial Gluconobacter processes is discussed. The impact of recombinant gene technology on the status of Gluconobacter processes and the potential use of such techniques in clarifying aspects of the physiology of Gluconobacter is reviewed.

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Industrial Production of l-Ascorbic Acid (Vitamin C) and d-Isoascorbic Acid

Pappenberger¹,

Hohmann²

2013

Advances in Biochemical Engineering/Biotechnology

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L-ascorbic acid (vitamin C) was first isolated in 1928 and subsequently identified as the long-sought antiscorbutic factor. Industrially produced L-ascorbic acid is widely used in the feed, food, and pharmaceutical sector as nutritional supplement and preservative, making use of its antioxidative properties. Until recently, the Reichstein-Grüssner process, designed in 1933, was the main industrial route. Here, D-sorbitol is converted to L-ascorbic acid via 2-keto-L-gulonic acid (2KGA) as key intermediate, using a bio-oxidation with Gluconobacter oxydans and several chemical steps. Today, industrial production processes use additional bio-oxidation steps with Ketogulonicigenium vulgare as biocatalyst to convert D-sorbitol to the intermediate 2KGA without chemical steps. The enzymes involved are characterized by a broad substrate range, but remarkable regiospecificity. This puzzling specificity pattern can be understood from the preferences of these enyzmes for certain of the many isomeric structures which the carbohydrate substrates adopt in aqueous solution. Recently, novel enzymes were identified that generate L-ascorbic acid directly via oxidation of L-sorbosone, an intermediate of the bio-oxidation of D-sorbitol to 2KGA. This opens the possibility for a direct route from D-sorbitol to L-ascorbic acid, obviating the need for chemical rearrangement of 2KGA. Similar concepts for industrial processes apply for the production of D-isoascorbic acid, the C5 epimer of L-ascorbic acid. D-isoascorbic acid has the same conformation at C5 as D-glucose and can be derived more directly than L-ascorbic acid from this common carbohydrate feed stock.

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Metabolism ofL-sorbose by enzymes fromGluconobacter melanogenus IFO 3293

Cited by 8 publications

References 9 publications

Metabolic Pathway for 2-Keto-l-gulonic Acid Formation inGluconohacter melanogenusIFO 3293

Metabolic Pathway for 2-Keto-l-gulonic Acid Formation inGluconohacter melanogenusIFO 3293

The GenusGluconobacterand Its Applications in Biotechnology

Industrial Production of l-Ascorbic Acid (Vitamin C) and d-Isoascorbic Acid

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