Metabolism studies of benzbromarone in rats by high performance liquid chromatography–quadrupole time of flight mass spectrometry

Wang, Haiqing; Peng, Ying; Wang, Shaojie; Wang, Kai; Zhao, Xunchen; Jiang, Fan

doi:10.1016/j.jchromb.2012.10.035

Cited by 9 publications

(2 citation statements)

References 18 publications

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“…The supernatants were harvested, and the ACN was evaporated under a stream of nitrogen gas at 40°C. The resulting urine samples were extracted with ethyl acetate (Wu et al, 2012). The organic layer was collected and evaporated to dryness under a stream of nitrogen gas at 40°C.…”

Section: Introductionmentioning

confidence: 99%

Identification of Epoxide-Derived Metabolite(s) of Benzbromarone

Wang

Peng

et al. 2016

Drug Metabolism and Disposition

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Benzbromarone (BBR) is a benzofuran derivative that has been quite useful for the treatment of gout; however, it was withdrawn from European markets in 2003 because of reported serious incidents of drug-induced liver injury. BBR-induced hepatotoxicity has been suggested to be associated with the formation of a quinone intermediate. The present study reported epoxide-derived intermediate(s) of BBR. An N-acetylcysteine (NAC) conjugate derived from epoxide metabolite(s) was detected in both microsomal incubations of BBR and urine samples of mice treated with BBR. The NAC conjugate was identified as 6-NAC BBR. Ketoconazole suppressed the bioactivation of BBR to the epoxide intermediate(s), and the CYP3A subfamily was the primary enzyme responsible for the formation of the epoxide(s). The present study provided new information on metabolic activation of BBR.

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Section: Introductionmentioning

confidence: 99%

Identification of Epoxide-Derived Metabolite(s) of Benzbromarone

Wang

Peng

et al. 2016

Drug Metabolism and Disposition

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“…Since these compounds are proposed for the management of HLB, the detection and evaluation of their concentration is critical. Previous studies have reported the quantification of benzbromarone in rats and human tissues using gas chromatography-mass spectrometry (GC-MS) and highperformance liquid chromatography-quadruple time of flight mass spectrometry (HPLC-Q-TOF), respectively (Beyer et al, 2005;Wu et al, 2012). Beyer et al (2005) used GC-MS analysis methods for the detection of benzbromarone with an estimated value of 0.05 mg/L for LOD in human urine.…”

Section: Discussionmentioning

confidence: 99%

Method Optimization: Analysis of Benzbromarone and Tolfenamic Acid in Citrus Tissues and Soil Using Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry

et al. 2020

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Herein, an analytical method was developed for extraction and quantification of benzbromarone and tolfenamic acid in citrus and soil matrices using liquidliquid extraction followed by liquid chromatography-triple quadrupole-tandem mass spectrometry analysis. The compounds were extracted using 0.1% formic acid in 6:4 ethyl acetate and n-hexane solution, and the analytes were separated using a mixture of 0.1% formic acid in ultrapure water and 0.1% formic acid in acetonitrile as mobile phase. A six-point in-matrix calibration curve was constructed providing good linearity with coefficients of determination R 2 ≥ 0.98. The limits of detection and quantification for benzbromarone and tolfenamic acid were 3.0 and 10.0 µg/kg in roots, peel, juice, and soil, and 4.0 and 12.0 µg/kg for leaves samples, respectively. The method yielded excellent recoveries between 81.3 and 101.2%, with relative standard deviation ≤9.5% in the matrices. The developed technique provides a simple and sensitive method for the determination of the chemicals and can be applied to agricultural practices.

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In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS

Xue

et al. 2014

Chromatographia

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Metabolism studies of benzbromarone in rats by high performance liquid chromatography–quadrupole time of flight mass spectrometry

Cited by 9 publications

References 18 publications

Identification of Epoxide-Derived Metabolite(s) of Benzbromarone

Identification of Epoxide-Derived Metabolite(s) of Benzbromarone

Method Optimization: Analysis of Benzbromarone and Tolfenamic Acid in Citrus Tissues and Soil Using Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry

In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS

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