Métabolisme rénal du palmitate au cours de la période périnatale chez le rat

Delaval, Evelyne; Freund, Nicole; Andriamanantsara, S.; Geloso, J. P.

doi:10.1051/rnd:19850225

Cited by 2 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This enhanced enzyme activity is not related to changes in kinetics in our assay system, and could reflect a synthesis of f,hydroxyacyl-CoA dehydrogenase. Therefore, in addition to carnitine palmitoyltransferase, whose activity is very low at 21 days post coitum [17], the activity of other fl-oxidation-pathway enzymes could limit palmitate oxidation capacities in the developing rat kidney. The evolution of fumarase activity suggests a significant increase in the tricarboxylic acid-cycle activity between the fetal stage and 1 day postpartum: this could, in part, be responsible for the maturation ofmitochondrial respiratory functions.…”

Section: Discussionmentioning

confidence: 99%

Effects of birth on energy metabolism in the rat kidney

Bastin¹,

Delaval²,

Freund³

et al. 1988

Biochemical Journal

View full text Add to dashboard Cite

The oxygen-consumption rates and the activities of fumarase and beta-hydroxyacyl-CoA dehydrogenase were compared in mitochondria isolated from fetal- and neonatal-rat kidney. Whole-organ ATP, phosphocreatine and creatine contents were determined in parallel. Kidney mitochondrial respiratory rates in the presence of succinate, glutamate/malate and palmitoyl-L-carnitine increased between 21 days post coitum and 1 day post partum, together with activities of oxidative enzymes. However, this postnatal maturation of oxidative metabolism was not yet initiated in mitochondria isolated from kidney 1 h post partum. An increase in ATP and phosphocreatine was observed immediately after delivery; newborn-rat kidney ATP content then remained high, whereas phosphocreatine reserves decreased considerably between 6 h and 1 day post partum. It is concluded that the increase in high-energy phosphate compounds observed at birth is not initially related to an activation of oxidative phosphorylation, and probably involves a transient stimulation of anaerobic glycolysis, while a progressive mitochondrial maturation takes place in the rat kidney during the first day of newborn life.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of birth on energy metabolism in the rat kidney

Bastin¹,

Delaval²,

Freund³

et al. 1988

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…After birth, the use of fatty acids as energy fuel by the immature kidney depends on the development of key enzymes of the mitochondrial β-oxidation pathway. Indeed, marked increases in the activities of carnitine palmitoyltransferase [12] and β-hydroxyacyl-CoA dehydrogenase [13] occur in the rat kidney during the postnatal period. However, the molecular mechanisms and signals involved in the postnatal induction of β-oxidation enzyme expression in the kidney and in other organs have not yet been delineated.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation by glucocorticoids of mitochondrial oxidative enzyme genes in the developing rat kidney

Djouadi

Bastin

Kelly

et al. 1996

Biochemical Journal

View full text Add to dashboard Cite

Mitochondrial fatty acid beta-oxidation plays a major role in providing the ATP required for reabsorptive processes in the adult rat kidney. However, the molecular mechanisms and signals involved in induction of the enzymes of fatty acid oxidation during development in this and other organs are unknown. We therefore studied the changes in the steady-state levels of mRNA encoding medium-chain acyl-CoA dehydrogenase (MCAD), which catalyses the initial step in mitochondrial fatty acid beta-oxidation, in the rat kidney cortex and medulla between postnatal days 10 and 30. Furthermore, we investigated whether the expression of MCAD and of mitochondrial malate dehydrogenase (mMDH), a key enzyme in the tricarboxylic acid cycle, might be co-ordinately regulated by circulating glucocorticoids in the immature kidney during development. In the cortex, the levels of MCAD mRNA rose 4-fold between day 10 and day 21, and then decreased from day 21 to day 30. In the medulla a postnatal increase in the concentration of MCAD mRNA (8-fold) was observed during the same period. Adrenalectomy prevented the 16-21-day developmental increases in MCAD and mMDH mRNA levels in the cortex and medulla; these could be restored by dexamethasone treatment. A single injection of dexamethasone into 10-day-old rats led to a rise in MCAD and mMDH mRNA levels in the renal cortex due to stimulation of gene transcription, as shown by nuclear run-on assays. Therefore MCAD and mMDH gene expression is tightly regulated at the transcriptional level by developmental changes in circulating glucocorticoid levels. These hormones might thus represent a good candidate as a co-ordinating factor in the expression of nuclear genes encoding mitochondrial enzymes in the kidney during postnatal development.

show abstract

Métabolisme rénal du palmitate au cours de la période périnatale chez le rat

Cited by 2 publications

References 1 publication

Effects of birth on energy metabolism in the rat kidney

Effects of birth on energy metabolism in the rat kidney

Transcriptional regulation by glucocorticoids of mitochondrial oxidative enzyme genes in the developing rat kidney

Contact Info

Product

Resources

About