Metabolite Bioactive Contents of Parkia timoriana (DC) Merr Seed Extracts in Different Solvent Polarities

Abstract

Parkia roxburghii G.Don seeds have popular folkloric ethnomedicinal use in the treatment of many diseases especially in Indonesia. Methanol, distilled water, n-hexane, and ethyl acetate extracts from the seeds of Parkia roxburghii were assayed for secondary metabolism quantitative, antioxidant, antidiabetic and antibacterial and activities as well as determined the presence of phytochemical constituents. The extracts were investigated for antioxidant possession by DPPH free-radical (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’ Azinobis (3-ethylbenz-thiazoline-6-sulfonic-acid) scavenging ability, Antidiabetic potential was invitro assayed by α-amylase inhibition and α-glucosyde inhibition while antibacterially by applying the disk diffusion procedure, as well as refined for the attendance of bioavailable phytochemical components. The result showed the existence of phytochemical components in diverse extracts could attribution free scavenging, antidiabetic and antimicrobial activities. The qualitative results all extracts of Parkia roxburghii seeds have expressed the presence of alkaloid, flavonoid, steroid, terpenoids, saponins and tannin whereas, methanol, distilled water, and n-hexane extracts expressed the presence of anthraquinones, The entire phenolic contents were examined as attested by the Follin-Ciocalteu methods, which varied from 43.82 - 137.42 mg GAE/g. The entire flavonoid compounds were measured with aluminum chloride colorimetric procedure, which varied from 20.42 – 45.90 mg QEDW/g. The total phenolic compound were measure Follin Ciocalteu which varied from 43.82-137.42 mg/g. The alkaloid, saponin, tanic acid, terpenoid and cardiac glycoside quantitative were measured with spectrofotometri UV-VIS which varied from 16.34 – 48.90 mg for alkaloid. The Saponin content varied 1.76 – 16.04 mg/g. Tanic acid which varied 0.21 – 7.29 mg/g. Terpenoid which varied 50.12 – 91.02 mg/g. Cardiac glycoside which varied 7.24-36.53 mg/g. The potential antioxidant were measured with ABTS and DPPH method, the methanol extract is the potential antioxidant. Antidiabetic potential were measured with alfa amylase and alfa glucosyde inhibition, the best antidiabetic is methanol extract. The potential antibacterial and antifungal was the methanol extract for Eschericia Coli and Candida Albicans. The conclusion established the tremendous perspective of the Parkia roxburghii seeds as another option fountain of food supplement, as well as drug components.

Plants as herbal medicine at Nguter Traditional Market, Sukoharjo, Central Java, Indonesia

“…According to Suryanti et al (2022), dawung (P. timoriana) phytochemically found alkaloids, saponins, tannins, flavonoids, and triterpenoids by treating the seeds with different extracts using standard procedures. Dawung (P. timoriana) is efficacious as a medicine for flatulence, while the leaves are efficacious as a cough medicine.…”

Chemical compositions, antioxidant and antibacterial activities of kepel (Stelechocarpus burahol) fruit flesh and peel extracts

“…The antioxidant activity is affected by the number and the arrangement of the hydroxyls on the aromatic ring. Naturally occurring antioxidant compounds are β-carotene, 1ascorbic acid, alkaloids, saponins, and tannins, as well as phenolics such as flavonoids, cinnamic acid derivates, coumarin, and tocopherols (Suryanti et al 2016;Suryanti et al 2021;Suryanti et al 2022). Secondary metabolite structure modification is frequently used to improve antioxidant activity (Suryanti et al 2018).…”

Antioxidant Activity of Microalgae Extract Cosmarium sp. Using 2.2-Azinobis-(3-Ethylbenzothiazoline)-6-Sulfonic Acid (ABTS) Radical Cation Assay

“…It was calculated by a linear regression equation between the percentage of inhibition and the sample concentration. IC50 was calculated from the equation : Note: a = intercept ; b = slop (Suryanti et al, 2022).…”

“…These were characterized as low molecular weight compounds with a nitrogen atom in the heterocyclic ring and had alkaline properties. (Suryanti et al, 2022). According to Chung & Woo (2001), alkaloids, especially indole, can efficiently stop free radical chain reactions.…”

“…The protonated N atom has a strong electron capacity. Extrusion in electron density affects the antioxidant stage since the electron transfer capacity (ET) is a critical component of an antioxidant (Suryanti et al, 2022;Xie et al, 2018). The extract did not contain phenolic compounds, as indicated by negative flavonoid and tannin results.…”