Metabolite Measurements

Roessner, Ute; Beckles, Diane M.

doi:10.1007/978-0-387-78745-9_3

Cited by 31 publications

(15 citation statements)

References 105 publications

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“…Hundreds and hundreds of compounds with different physical and chemical properties such as molecular weight, molecular size, polarity, stability, volatility, solubility, and many more, require the development of comprehensive and/or complementary extraction, separation, detection, and quantification techniques (Beckles and Roessner, 2012). A number of analytical technologies, including chromatographic separation techniques, such as liquid and gas chromatography, coupled to mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy have been successfully used to analyze metabolites in many different organisms, tissues and biofluids (Roessner and Beckles, 2009). …”

Section: Advancements In Functional Genomics Techniques For Spatial Rmentioning

confidence: 99%

Advances in functional genomics for investigating salinity stress tolerance mechanisms in cereals

Shelden¹,

Roessner²

2013

Front. Plant Sci.

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Abiotic stresses such as low water availability and high salinity are major causes of cereal crop yield losses and significantly impact on sustainability. Wheat and barley are two of the most important cereal crops (after maize and rice) and are grown in increasingly hostile environments with soil salinity and drought both expected to increase this century, reducing the availability of arable land. Barley and wheat are classified as glycophytes (salt-sensitive), yet they are more salt-tolerant than other cereal crops such as rice and so are good models for studying salt tolerance in cereals. The exploitation of genetic variation of phenotypic traits through plant breeding could significantly improve growth of cereals in salinity-affected regions, thus leading to improved crop yields. Genetic variation in phenotypic traits for abiotic stress tolerance have been identified in land races and wild germplasm but the molecular basis of these differences is often difficult to determine due to the complex genetic nature of these species. High-throughput functional genomics technologies, such as transcriptomics, metabolomics, proteomics, and ionomics are powerful tools for investigating the molecular responses of plants to abiotic stress. The advancement of these technologies has allowed for the identification and quantification of transcript/metabolites in specific cell types and/or tissues. Using these new technologies on plants will provide a powerful tool to uncovering genetic traits in more complex species such as wheat and barley and provide novel insights into the molecular mechanisms of salinity stress tolerance.

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Section: Advancements In Functional Genomics Techniques For Spatial Rmentioning

confidence: 99%

Advances in functional genomics for investigating salinity stress tolerance mechanisms in cereals

Shelden¹,

Roessner²

2013

Front. Plant Sci.

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“…A range of analytical technologies has been employed to analyze metabolites in different organisms, tissues, or fluids (for review see Reference 3). Mass spectrometry coupled to different chromatographic separation techniques, such as liquid or gas chromatography or NMR, are the major tools to analyze a large number of metabolites simultaneously.…”

Section: What Is Metabolomics?mentioning

confidence: 99%

“…Due to the huge diversity of chemical structures and the large differences in abundance, there is no single technology available to analyze the entire metabolome. Therefore, a number of complementary approaches have to be established for extraction, detection, quantification, and identification of as many metabolites as possible (3,4). …”

Section: What Is Metabolomics?mentioning

confidence: 99%

What is metabolomics all about?

2009

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“…A detailed description of these technologies and their advantages and disadvantages are presented in [4]. When MS is applied, often metabolites in complex mixtures need to be separated prior to analysis.…”

Section: Technologies Used To Measure Metabolitesmentioning

confidence: 99%