Metabolite-Mediated Responses of Phyllosphere Microbiota to Rust Infection in Two
            <i>Malus</i>
            Species

Zhang, Yunxia; Cao, Bin; Pan, Yumei; Tao, Siqi; Zhang, Nai‐Li

doi:10.1128/spectrum.03831-22

Cited by 8 publications

(5 citation statements)

References 100 publications

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“…The current study found that symptomatic leaves tend to be colonized by a higher number of fungal species, as evidenced by the significantly higher values obtained for fungal richness in symptomatic plants than in asymptomatic plants. A recent study also indicated that the alpha-diversity of fungal communities associated with crabapple species increases with the infection process by the rust fungus Gymnosporangium yamadae (Zhang et al, 2023). The increased fungal richness associated with the disease can be due to the fact that the pathogen interferes with the plant immune system, facilitating the entry of numerous microbes into the plant to compete for the available resources (Hu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Metagenomic insight to apprehend the fungal communities associated with leaf blight of Welsh onion in Taiwan

Jayasinghe,

Chang,

Knobloch

et al. 2024

Front. Plant Sci.

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Plants are associated with a large diversity of microbes, and these complex plant-associated microbial communities are critical for plant health. Welsh onion (Allium fistulosum L.) is one of the key and oldest vegetable crops cultivated in Taiwan. The leaf of the Welsh onion is one of the famous spices in Taiwanese cuisine, thus, it is crucial to control foliar diseases. In recent years, Welsh onion cultivation in Taiwan has been severely threatened by the occurrence of leaf blight disease, greatly affecting their yield and quality. However, the overall picture of microbiota associated with the Welsh onion plant is still not clear as most of the recent etiological investigations were heavily based on the isolation of microorganisms from diseased plants. Therefore, studying the diversity of fungal communities associated with the leaf blight symptoms of Welsh onion may provide information regarding key taxa possibly involved in the disease. Therefore, this investigation was mainly designed to understand the major fungal communities associated with leaf blight to identify key taxa potentially involved in the disease and further evaluate any shifts in both phyllosphere and rhizosphere mycobiome assembly due to foliar pathogen infection by amplicon sequencing targeting the Internal Transcribed Spacer (ITS) 1 region of the rRNA. The alpha and beta-diversity analyses were used to compare the fungal communities and significant fungal groups were recognized based on linear discriminant analyses. Based on the results of relative abundance data and co-occurrence networks in symptomatic plants we revealed that the leaf blight of Welsh onion in Sanxing, is a disease complex mainly involving Stemphylium and Colletotrichum taxa. In addition, genera such as Aspergillus, Athelia and Colletotrichum were abundantly found associated with the symptomatic rhizosphere. Alpha-diversity in some fields indicated a significant increase in species richness in the symptomatic phyllosphere compared to the asymptomatic phyllosphere. These results will broaden our knowledge of pathogens of Welsh onion associated with leaf blight symptoms and will assist in developing effective disease management strategies to control the progress of the disease.

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Section: Discussionmentioning

confidence: 99%

Metagenomic insight to apprehend the fungal communities associated with leaf blight of Welsh onion in Taiwan

Jayasinghe,

Chang,

Knobloch

et al. 2024

Front. Plant Sci.

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“…In other words, the diseased leaves experienced a depletion of their otherwise beneficial flora, which could result in reduced resistance to pathogenic bacteria. Microbial ecological networks play a crucial role in characterizing the relationships among microorganisms within a community, and recent evidence suggests that the characteristics of ecological networks can influence the community's response to pathogen invasion [16,22]. Modulization within the network enhances its stability when faced with disturbances [78].…”

Section: Small Changes In Host Microbiome Composition Predict Disease...mentioning

confidence: 99%

“…However, this study overlooked the changing patterns of microbial communities as disease processes. Another study analyzed the phyllosphere microbial communities in G. yamadae-infected crabapple leaves from field sampling at six developmental stages using amplicon-based methods and revealed that the pattern of microbial communities varied between Malus varieties [22]. However, it is important to note that natural diseases in the field exhibit randomness, making it difficult to ensure consistent sources of pathogens within samples, consistent levels of pathogen invasion, and the identification of disease progression stages.…”

Section: Introductionmentioning

confidence: 99%

Time-Course Responses of Apple Leaf Endophytes to the Infection of Gymnosporangium yamadae

Li,

Tao,

Liang

2024

JoF

Self Cite

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Apple rust, caused by Gymnosporangium yamadae, poses a significant challenge to apple production. Prior studies have underscored the pivotal role played by endophytic microbial communities, intimately linked with the host, in influencing plant diseases and their pathogenic outcomes. The objective of this study is to scrutinize alternations in endophytic microbial communities within apple leaves at different stages of apple rust using high-throughput sequencing technology. The findings revealed a discernible pattern characterized by an initial increase and subsequent decrease in the alpha diversity of microbial communities in diseased leaves. A microbial co-occurrence network analysis revealed that the complexity of the bacterial community in diseased leaves diminished initially and then rebounded during the progression of the disease. Additionally, employing the PICRUSt2 platform, this study provided preliminary insights into the functions of microbial communities at specific disease timepoints. During the spermogonial stage, endophytic bacteria particularly exhibited heightened activity in genetic information processing, metabolism, and environmental information processing pathways. Endophytic fungi also significantly enriched a large number of metabolic pathways during the spermogonial stage and aecial stage, exhibiting abnormally active life activities. These findings establish a foundation for comprehending the role of host endophytes in the interaction between pathogens and hosts. Furthermore, they offer valuable insights for the development and exploitation of plant endophytic resources, thereby contributing to enhanced strategies for managing apple rust.

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“…Moreover, certain metabolites such as coumarins, flavonoids, lignin precursors, quaternary ammonium salts, and terpenoids were enriched with the expansion of the lesion and had positive regulatory effects on Rhynchogastremataceae, Golubeviaceae, and Actinomycetales, playing a significant role in the assembly of phyllosphere microbial communities [31]. Plant hormones can also act as a selective force in the microbiome assembly process.…”

Section: Leaf Exudatesmentioning

confidence: 99%

Phyllosphere Microbiome in Plant Health and Disease

De Mandal,

Jeon

2023

Plants

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The phyllosphere refers to the aboveground surface of plants colonized by diverse microorganisms. Microbes inhabiting this environment play an important role in enhancing the host’s genomic and metabolic capabilities, including defense against pathogens. Compared to the large volume of studies on rhizosphere microbiome for plant health and defense, our understanding of phyllosphere microbiome remains in its infancy. In this review, we aim to explore the mechanisms that govern the phyllosphere assembly and their function in host defence, as well as highlight the knowledge gaps. These efforts will help develop strategies to harness the phyllosphere microbiome toward sustainable crop production.

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Metabolite-Mediated Responses of Phyllosphere Microbiota to Rust Infection in Two Malus Species

Cited by 8 publications

References 100 publications

Metagenomic insight to apprehend the fungal communities associated with leaf blight of Welsh onion in Taiwan

Metagenomic insight to apprehend the fungal communities associated with leaf blight of Welsh onion in Taiwan

Time-Course Responses of Apple Leaf Endophytes to the Infection of Gymnosporangium yamadae

Phyllosphere Microbiome in Plant Health and Disease

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