Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract

Corradi, Elisabetta; Schmidt, Nadine; Räber, N; Mieri, Maria De; Hamburger, Matthias; Butterweck, Veronika; Potterat, Olivier

doi:10.1055/s-0043-109098

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…Peak 4 at 15.8 min corresponded to the known compound triandrin, in which m / z 357.1187 arises from its formate adduct and 311.1133 from its molecular ion [15]. Triandrin ( 4 ) has been recently found in the leaves and twigs of S. reticulata [16], but is better known in S. triandra [6,7], a parent of NWC1283. Compound 5 eluted at 16.3 min and showed m / z 371.1347 [M − H + HCOOH] − .…”

Section: Resultsmentioning

confidence: 99%

Phenylalkanoid Glycosides (Non-Salicinoids) from Wood Chips of Salix triandra × dasyclados Hybrid Willow

Dias

Bellisai

et al. 2019

Molecules

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Salix triandra (almond leaved willow) is an established crop, grown in coppicing regimes for basket-making materials. It is known as a source of non-salicinoid phenolic glycosides, such as triandrin and salidroside. A spontaneous natural hybrid of S. triandra and S. dasyclados was subjected to metabolite profiling by high resolution LC-MS, and 22 phenolic glycosides, including 18 that are new to the Salicaceae, were identified. Structures were determined by HPLC isolation and NMR methods. The hybridisation process has introduced novel chemistry into the Salix phenolic glycoside palette, in particular, the ability to generate disaccharide conjugates where the glycosyl group is further extended by a range of sugars, including apiose, rhamnose, xylose, and arabinose. Also of note is the appearance of chavicol derivatives, also not previously seen in Salix spp. The work demonstrates the plasticity of the phenolic glycoside biosynthetic pathway, and the potential to improve established crops such as S. triandra and S. dasyclados, via high-value metabolites, for both basketry and bioenergy markets.

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Section: Resultsmentioning

confidence: 99%

Phenylalkanoid Glycosides (Non-Salicinoids) from Wood Chips of Salix triandra × dasyclados Hybrid Willow

Dias

Bellisai

et al. 2019

Molecules

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“…Using n-butanol as a solvent resulted in the separation of picein with 93.6% purity [ 93 ]. Another study performed on immortalized human non-tumorigenic keratinocytes (HaCaT) also showed that the other compounds of Salix reticulata significantly inhibited cell proliferation, whereas such an effect was not observed for picein [ 94 ]. They also used the MeOH extract from aerial parts and showed that MeOH extract with a concentration of 100 µg/ml reduced cell viability by about 60%, but the separation of the flavonoids and compounds failed to show any effect on cell proliferation for picein [ 94 ].…”

Section: Antioxidative and Neuroprotective Properties Of Piceinmentioning

confidence: 99%

“…Another study performed on immortalized human non-tumorigenic keratinocytes (HaCaT) also showed that the other compounds of Salix reticulata significantly inhibited cell proliferation, whereas such an effect was not observed for picein [ 94 ]. They also used the MeOH extract from aerial parts and showed that MeOH extract with a concentration of 100 µg/ml reduced cell viability by about 60%, but the separation of the flavonoids and compounds failed to show any effect on cell proliferation for picein [ 94 ]. Other compounds of Salix pseudo-lasiogyne twigs not including picein were also identified to have inhibitory effects against lipopolysaccharide-induced NO production in vitro (on microglial cells) and also significant antioxidative effects on lutathione reductase and superoxide dismutase production in vivo (on scopolamine-induced mice) [ 95 ].…”

Section: Antioxidative and Neuroprotective Properties Of Piceinmentioning

confidence: 99%

The Antioxidative Effects of Picein and Its Neuroprotective Potential: A Review of the Literature

et al. 2022

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Neurodegenerative diseases (NDDs) are the main cause of dementia in the elderly, having no cure to date, as the currently available therapies focus on symptom remission. Most NDDs will progress despite treatment and eventually result in the death of the patient after several years of a burden on both the patient and the caregivers. Therefore, it is necessary to investigate agents that tackle the disease pathogenesis and can efficiently slow down or halt disease progression, with the hope of curing the patients and preventing further burden and mortality. Accordingly, recent research has focused on disease-modifying treatments with neuroregenerative or neuroprotective effects. For this purpose, it is necessary to understand the pathogenesis of NDDs. It has been shown that oxidative stress plays an important role in the damage to the central nervous system and the progression of neurodegenerative disorders. Furthermore, mitochondrial dysfunction and the accumulation of unfolded proteins, including beta-amyloid (Aβ), tau proteins, and α-synuclein, have been suggested. Accordingly, cellular and molecular studies have investigated the efficacy of several natural compounds (herbs and nutritional agents) for their neuroprotective and antioxidative properties. The most popular herbs suggested for the treatment and/or prevention of NDDs include Withania somnifera (ashwagandha), ginseng, curcumin, resveratrol, Baccopa monnieri, and Ginkgo biloba. In some herbs, such as ginseng, preclinical and clinical evidence are available for supporting its effectiveness; however, in some others, only cellular and animal studies are available. In line with the scant literature in terms of the effectiveness of herbal compounds on NDDs, there are also other herbal agents that have been disregarded. Picein is one of the herbal agents that has been investigated in only a few studies. Picein is the active ingredient of several herbs and can be thus extracted from different types of herbs, which makes it more available. It has shown to have anti-inflammatory properties in cellular and plant studies; however, to date, only one study has suggested its neuroprotective properties. Furthermore, some cellular studies have shown no anti-inflammatory effect of picein. Therefore, a review of the available literature is required to summarize the results of studies on picein. To date, no review study seems to have addressed this issue. Thus, in the present study, we gather the available information about the antioxidative and potential neuroprotective properties of picein and its possible effectiveness in treating NDDs. We also summarize the plants from which picein can be extracted in order to guide researchers for future investigations.

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“…The protocol was similar to that described previously with some modifications [11,13,27]. The inserts for cell culture were positioned in 12-well plates.…”

Section: Wound-scratch Assay (Cell Migration Assay)mentioning

confidence: 99%

Antiproliferative Effects of Pterodon pubescens Extract and Isolated Diterpenes in HaCaT Cells

et al. 2020

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Pterodon pubescens fruits are popularly used because of their analgesic and anti-inflammatory actions, which are attributed to the isolated compounds with a vouacapan skeleton. This work aimed to evaluate the antiproliferative and anti-inflammatory effects of a P. pubescens fruit dichloromethane extract and the vouacapan diterpene furan isomerʼs mixture (1 : 1) (6α-hydroxy-7β-acetoxy-vouacapan-17β-oate methyl ester and 6α-acetoxy-7β-hydroxy-vouacapan-17β-oate methyl ester isomers) in HaCaT cells using the cell migration and the BrDU incorporation assay. Levels of IL-8 were measured by ELISA after TNF-α stimulation. HPLC/DAD analysis of the extract revealed the expressive presence of vouacapan diterpene furan isomerʼs mixture. P. pubescens extract (1.5625 – 25 µg/mL) and vouacapan diterpene furan isomerʼs mixture (3.125 – 50 µM) inhibited cell proliferation as indicated by a decreased BrdU-incorporation. For the evaluation of cell migration, time-lapse microscopy was used. P. pubescens presented inhibition on cell migration at all concentrations tested (3.125 – 12.5 µg/mL), whereas for the VDFI mixture, the inhibition was only observed at the highest concentrations (12.5 and 25 µM) tested. Furthermore P. pubescens extract and vouacapan diterpene furan isomerʼs mixture significantly decreased IL-8 levels. Our results showed antiproliferative and anti-inflammatory effects on HaCaT cells treated with the extract and the vouacapan isomerʼs mixture, without affecting cell viability. These activities could be attributed to the voucapan molecular structures. In conclusion, topical products developed of P. pubescens extract or the voucapan isomerʼs mixture should be further studied as a potential product for local treatment against hyperproliferative lesions as in psoriasis vulgaris, representing an alternative treatment approach.

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Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract

Cited by 3 publications

References 31 publications

Phenylalkanoid Glycosides (Non-Salicinoids) from Wood Chips of Salix triandra × dasyclados Hybrid Willow

Phenylalkanoid Glycosides (Non-Salicinoids) from Wood Chips of Salix triandra × dasyclados Hybrid Willow

The Antioxidative Effects of Picein and Its Neuroprotective Potential: A Review of the Literature

Antiproliferative Effects of Pterodon pubescens Extract and Isolated Diterpenes in HaCaT Cells

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