Metabolite profile of marine-derived endophytic Streptomyces sundarbansensis WR1L1S8 by liquid chromatography-mass spectrometry and evaluation of culture conditions on antibacterial activity and mycelial growth

Abstract

Aims: This study was designed to investigate whether culture conditions (media, seawater concentration and pH) could lead Streptomyces sundarbansensis strain (isolated from marine brown algae Fucus sp. collected from Algerian coastline) to produce bioactive secondary metabolites. The most favourable condition for the production of anti-MRSA compound 1 [2-hydroxy-5-((6-hydroxy-4-oxo-4H-pyran-2-yl)methyl)-2-propylchroman-4-one] was determined. Methods and Results: The profile of metabolites present in the crude extracts was carried out by HPLC analysis equipped with a diode array detector evaporative light scattering detection (DAD-ELSD) or online coupled to electrospray ionization-mass spectrometry (ESI-MS). Compound 1 was the most abundant secondary metabolite by culturing the strains on starch casein agar (SCA) medium in freshwater or 50% seawater at pH 7 or 9 using agar-state fermentation method. Conclusions:The study has shown the efficiency of HPLC/ESI-MS technique in the analysis of polyketides produced by the strain under investigation. It was possible to establish the best culture conditions for obtaining the most bioactive compound 1, previously isolated by the same strain. Significance and Impact of the Study: Marine algae-actinobacteria associations are a particularly promising renewable system for the production of new antibacterial metabolites. Based on the promising bioactivity of the chemically characterized compound 1, the analytical methodology here applied has resulted as an effective approach for establishing its optimized production.

Biological Significance of Marine Actinobacteria of East Coast of Andhra Pradesh, India

“…Earlier findings also illustrated ISP-2 (Rateb et al, 2014) and starch casein as suitable media for the production of secondary metabolites by Streptomyces spp. (Djinni et al, 2013). …”

Antifungal Molecules Produced by a New Salt-Tolerant and Alkaliphilic Streptomyces sp. BS30 Isolated from an Arid Soil

“…Thus, the molecules obtained by the authors are natural, non-polyenic and antifungal. The HPLC/ESI-MS technique is important and reliable in the analysis of antimicrobial metabolites produced by the Streptomyces strains as demonstrated recently by Djinni et al [35].…”

Characterization of Culturable Actinomycetes Associated with Halophytic Rhizosphere as Potential Source of Antibiotics

“…The shapes of the spore morphology of the identified streptomycete isolates were studied using a Field Emission Scanning Electron Microscopy (FESEM-SUPRA 55, Carl Zeiss, Germany). The cultural characteristics were studied by the standard methods of International Streptomyces Project (ISP) [31]. NaCl tolerance, carbon source utilization, growth at different pH and temperatures were tested on AIM medium with 14 days incubations.…”