Metabolite profiling from different parts of tender fruit endosperm of <i>Borassus flabellifer</i> L. (ice apple)

Vasudevan, Vinduja; Prabaharan, Janani; Krishnan, Nagasathiya; Abinaya, K.; Gopinath, Subash C.B.; Pachaiappan, Raman

doi:10.1039/d3ay00704a

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…By using LC-MS/MS-based peptide mass fingerprinting, the hydrolysates were analyzed, and proteins were identified by the Mascot Matrix Science. 25,26…”

Section: Lc-ms/ms-based Peptide Mass Fingerprintingmentioning

confidence: 99%

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HPLC purification of antioxidant and antibacterial peptides from a lichen “Parmotrema perlatum (Huds.) M. Choisy”: Identification by LC‐MS/MS peptide mass fingerprinting

Radhakrishnan,

Kanakaraja,

Krishnan

et al. 2024

Biotech and App Biochem

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Parmotrema perlatum, a lichen belonging to the family Parmeliaceae, is well known for its culinary benefits and aroma used as a condiment in Indian homes is also known as the “black stone flower” or “kalpasi” in India. This research intends to analyze the antioxidant power of the crude extracts using four pH‐based buffers solubilized proteins/peptides and RP‐HPLC fractions of P. perlatum obtained by purification. The proteins that were extracted from the four different buffers were examined using LC‐MS/MS‐based peptide mass fingerprinting. When compared to the other buffers, the 0.1 M of Tris‐HCl buffer pH 8.0 solubilized proteins/peptides had the strongest antioxidant capacity. The sequential purification of the peptide was carried out by using a 3‐kDa cut‐off membrane filter and semipreparative RP‐HPLC. Additionally, the purified fractions of the peptide's antioxidant activity were assessed, and effects were compared with those of the crude and 3 kDa cut‐‐off membrane filtrates. The peptide fractions were sequenced by LC‐MS/MS, which reveals that fraction 2 from RP‐HPLC with the sequence LSWFMVVAP has shown the highest antioxidant potential in comparison with other fractions which can serve as the potential natural antioxidant drug. Further, fraction 2 also showed antibacterial activity against the selected microorganisms.

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“…By using LC-MS/MS-based peptide mass fingerprinting, the hydrolysates were analyzed, and proteins were identified by the Mascot Matrix Science. 25,26…”

Section: Lc-ms/ms-based Peptide Mass Fingerprintingmentioning

confidence: 99%

“…The free amino acids histidine and arginine are reported to have potential anti-bacterial and anti-quorum-sensing activity. 26,34…”

Section: Quantification Of the Total Amino Acid From P Perlatummentioning

confidence: 99%

HPLC purification of antioxidant and antibacterial peptides from a lichen “Parmotrema perlatum (Huds.) M. Choisy”: Identification by LC‐MS/MS peptide mass fingerprinting

Radhakrishnan,

Kanakaraja,

Krishnan

et al. 2024

Biotech and App Biochem

Self Cite

View full text Add to dashboard Cite

show abstract

Characterization of phytoconstituents of vital herbal oils by GC–MS and LC–MS/MS and their bioactivities

Gopalakrishnan,

Singh,

Krishnan

et al. 2024

J Food Sci Technol

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Purification and characterization of 3-O-methyl-D-glucose from the seed coat of Vigna mungo (L.) Hepper

Singh,

Kannan,

Gopinath

et al. 2024

Process Biochemistry

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Metabolite profiling from different parts of tender fruit endosperm of Borassus flabellifer L. (ice apple)

Abstract: Borassus flabellifer L., commonly known as Asian palmyra, is native to the countries of South and Southeast Asia. The endosperms of B. flabellifer (known as nungu in the Dravidian culture)...

Cited by 3 publications

References 29 publications

HPLC purification of antioxidant and antibacterial peptides from a lichen “Parmotrema perlatum (Huds.) M. Choisy”: Identification by LC‐MS/MS peptide mass fingerprinting

HPLC purification of antioxidant and antibacterial peptides from a lichen “Parmotrema perlatum (Huds.) M. Choisy”: Identification by LC‐MS/MS peptide mass fingerprinting

Characterization of phytoconstituents of vital herbal oils by GC–MS and LC–MS/MS and their bioactivities

Purification and characterization of 3-O-methyl-D-glucose from the seed coat of Vigna mungo (L.) Hepper

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