Metabolite profiling of Drynariae Rhizoma using 1H NMR and HPLC coupled with multivariate statistical analysis

The Development and Validation of Simultaneous Multi-Component Quantitative Analysis via HPLC–PDA Detection of 12 Secondary Metabolites Isolated from Drynariae Rhizoma

“…DR is a herb that has widely been used in folk medicine for thousands of years [3]. Research has demonstrated that DR is rich in phenols, such as naringin, neoeriocitrin, and 5,7-dihydroxychromone-7-O-neohesperidoside, and contains phenylpropanoids, such as caffeic acid 4-O-β-d-glucoside and coumaric acid 4-O-β-d-glucoside [4]. Naringin, the main component of DR, possesses antioxidant, anti-inflammatory, hepatoprotective, and neuroprotective properties [5].…”

“…Yang et al (2015), in an investigation of potential treatment options for Alzheimer's disease, isolated naringin, neoeriocitrin, 5,7-dihydroxychromone-7-O-neohesperidoside, caffeic acid-4-O-β-D-glucoside, and protocatechuic acid from DR and performed HPLC qualitative analysis [6]. In a metabolite profiling study of DR, Dong et al (2023) performed an HPLC-based quantitative analysis of seven phenolic compounds, including naringin, neoeriocitrin, 5,7-dihydroxychromone-7-O-neohesperidoside, caffeic acid 4-O-β-d-glucoside, and coumaric acid 4-O-β-d-glucoside, which are major secondary metabolites of D. fortunei [4]. To date, previous HPLC quantitative analyses of DR have only simultaneously analyzed five or fewer compounds and have not secured reliability through validation; moreover, some multi-component simultaneous analyses have produced incomplete chromatograms.…”