Metabolite Profiling of Hydroxycinnamate Derivatives in Plasma and Urine after the Ingestion of Coffee by Humans: Identification of Biomarkers of Coffee Consumption

Abstract

ABSTRACT:Human subjects drank coffee containing 412 mol of chlorogenic acids, and plasma and urine were collected 0 to 24 h after ingestion and were analyzed by high-performance liquid chromatographymass spectrometry. Within 1 h, some of the components in the coffee reached nanomole peak plasma concentrations (C max ), whereas chlorogenic acid metabolites, including caffeic acid

Impact of dose on the bioavailability of coffee chlorogenic acids in humans

“…in healthy subjects and volunteers with an ileostomy, and allowed distinguishing between events occurring in the small and large intestine. 7,8 The low nM levels of CQAs and FQAs in plasma is also supported by other studies which have involved enzymic deglucuronidation and desulfation of the plasma samples. 9 The aim of the current study was to expand the earlier work of Stalmach et al 7,8 and to investigate the impact on the plasma pharmacokinetic profiles and urinary excretion of chlorogenic acid-derived metabolites after the acute ingestion by human volunteers of three instant coffees containing 412, 635 and 795 mol of chlorogenic acids per 200 mL serving.…”

Phytochemical uptake following human consumption of Montmorency tart cherry (L. Prunus cerasus) and influence of phenolic acids on vascular smooth muscle cells in vitro

“…participants a single serving of a coffee beverage fortified with CHL, the serving consisted of low (412 μmol), CHL that was found the MC concentrate that represents a sensible portion to consume, rather than an artificially 309 derived concentration used previously [34,35]. In support of this, a previous study using comparable…”

Effect of edible oils on quercetin, kaempferol and galangin transport and conjugation in the intestinal Caco-2/HT29-MTX co-culture model

“…The system has been used previously to examine metabolism of phenolics, where the metabolism of ferulic acid is mainly by sulphation both in vivo 22 and in the co-cultured cellular system 17 . These conjugation properties are due to the distribution and expression of conjugating enzyme isoforms.…”

“…Prior to the experiments, the culture medium was replaced by fresh medium for at least [12][13][14][15][16][17][18][19][20][21][22][23][24] hours. Transport experiments were carried out with transport buffer solution consisting of HBSS supplemented with 1.8 mM CaCl 2 (to maintain integrity of the tight junction) and 100 µM ascorbic acid (to minimise degradation of flavonols).…”