Metabolite profiling of the undifferentiated cultured cells and differentiated leaf tissues of Centella asiatica

Ncube, Efficient N.; Steenkamp, Paul A.; Madala, Ntakadzeni E.; Dubery, Ian A.

doi:10.1007/s11240-017-1189-4

Cited by 14 publications

(5 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tan et al also reported 5- O -feruloylquinic acid in I. batatas leaves. 5-Feruloylquinic acid was also quantified in a methanolic extract of C. asiatica using an ultrahigh-performance liquid chromatography (UHPLC)–mass spectrometry (MS) platform . Except for M. frondosa, T. triangulare, A. ficoidea, and C. aconitifolius, all the TLVs recorded an abundance of 5- O -feruloylquinic acid.…”

Section: Resultsmentioning

confidence: 99%

Valorization of Unconventional Traditional Leafy Vegetable Diversity for Minerals, Health-Promoting Phytochemicals, and Antioxidants

Anju,

Ahmad,

Ramchiary

et al. 2023

ACS Food Sci. Technol.

View full text Add to dashboard Cite

Traditional leafy vegetables (TLVs) are rich in beneficial bioactive compounds and mineral elements. The rural areas of Kerala, India, have a diversity of unconventional TLVs, and only limited research is available on their nutritional and biochemical composition. Therefore, this study was designed to evaluate the mineral elements, metabolites, antioxidants, antioxidant activity, and proximate composition of 14 TLVs. The results show that among the selected TLVs, Talinum triangulare exhibited the highest Zn (1214 mg/kg DW) content, whereas the highest amount of Ca (13,532 mg/kg DW) was found in Centella asiatica. GC−MS analysis of these 14 plants unveiled a total of 193 metabolites. The metabolic pathways with high impact were associated with alanine, aspartate, glutamate, galactose, and glyoxylate−dicarboxylate metabolism and aminoacyl tRNA synthesis. Several metabolites showed differential expression across the samples. Celosia argentia, Ipomoea batatas (sweetheart cultivar), and Ipomoea aquatica showed the highest expression of metabolites such as caffeic acid and xylitol. I. batatas cv. sidekick black (0.49 mg/mL) and Cnidoscolus aconitifolius (0.135 mg/mL) demonstrated the highest antioxidant activities. The 14 plants had varying levels of fiber (2.01−7.31%), fat (2.68−9.36%), protein (4.55−22.32%), and carbohydrate (34.62−69.3%). Overall, these TLVs have high nutritional potential and health-protecting properties, which can boost the well-being of consumers.

show abstract

Section: Resultsmentioning

confidence: 99%

Valorization of Unconventional Traditional Leafy Vegetable Diversity for Minerals, Health-Promoting Phytochemicals, and Antioxidants

Anju,

Ahmad,

Ramchiary

et al. 2023

ACS Food Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…13 This might be explained by the phenomenon of restricted synthesis of some secondary plant metabolites by specialized cells within tissues/organs and associated with a certain stage of development. 20 Allelopathy involves the production and release of bioactive secondary metabolites (i.e. allelochemicals) by a plant species that interfere with the growth of another plant species.…”

Section: Resultsmentioning

confidence: 99%

Lipopolysaccharides trigger synthesis of the allelochemical sorgoleone in cell cultures of Sorghum bicolor

Mareya

Tugizimana

Steenkamp

et al. 2020

Plant Signaling & Behavior

View full text Add to dashboard Cite

The use of plant cell suspension culture systems has demonstrated to be highly suitable for metabolomics investigations of inducible defense responses. Here we report on sorghum cell suspension cultures that were elicited with purified lipopolysaccharides from the sorghum pathogen Burkholderia andropogonis, to activate metabolic pathways involved in the chemical defenses of the plant. Metabolomic analysis using liquid chromatography coupled to mass spectrometry identified a resorcinol phenolic lipid, annotated as sorgoleone, as one of the biomarkers associated with the LPS-induced response. Sorgoleone is a semiochemical and an allelochemical, synthesized by specialized root hair cells and the major component of the hydrophobic root exudate of sorghum. Its detection in undifferentiated cells might indicate a previously undescribed role for this phytochemical in plant defense responses.

show abstract

“…Recent advances in plant biotechnology enable us to culture plant cells, tissues, and organs instead of complete plants to produce valuable secondary metabolites [ 11 ]. Because the low productivity and instability of plant cultures and non-synthesis of few important compounds in undifferentiated cells, mass production of important bioactive molecules is considered commercially difficult by plant cell culture [ 12 ]. Keeping these issues in consideration, to produce pharmaceutical and nutraceutical secondary metabolites, the adventitious root culture in a large-scale bioreactor is regarded as a great approach.…”

Section: Adventitious Root Culturementioning

confidence: 99%

Root Cultures, a Boon for the Production of Valuable Compounds: A Comparative Review

Hussain

Abbas

Nazli

et al. 2022

Plants

View full text Add to dashboard Cite

Medicinal plants are an inevitable source of pharmaceutical drugs and most of the world population depends on these plants for health benefits. The increasing global demand for bioactive compounds from medicinal plants has posed a great threat to their existence due to overexploitation. Adventitious root and hairy root culture systems are an alternative approach to the conventional method for mass production of valuable compounds from medicinal plants owing to their rapid growth, biosynthetic and genetic stability. The main purpose of this review is to investigate the recent scientific research published worldwide on the application of adventitious and hairy root cultures to produce valuable compounds from medicinal plants. Furthermore, a comparison of adventitious root vs. hairy root cultures to produce valuable compounds has also been discussed. Various aspects such as medium composition, carbon source, pH, amount of macronutrients, optimization strategy, scale-up cultures, and use of biotic abiotic and nano-elicitors at various concentrations are the topic of discussion in this review. Several studies on adventitious and hairy root cultures of Polygonum multiflorum¸ Withania somnifera¸ Echinacea purpurea and Ajuga bracteosa have been discussed in detail which highlights the importance of elicitation strategies and bioreactor system, presenting commercial applications.

show abstract

Metabolite profiling of the undifferentiated cultured cells and differentiated leaf tissues of Centella asiatica

Cited by 14 publications

References 59 publications

Valorization of Unconventional Traditional Leafy Vegetable Diversity for Minerals, Health-Promoting Phytochemicals, and Antioxidants

Valorization of Unconventional Traditional Leafy Vegetable Diversity for Minerals, Health-Promoting Phytochemicals, and Antioxidants

Lipopolysaccharides trigger synthesis of the allelochemical sorgoleone in cell cultures of Sorghum bicolor

Root Cultures, a Boon for the Production of Valuable Compounds: A Comparative Review

Contact Info

Product

Resources

About