Metabolite Profiling to Characterize Disease-related Bacteria

Behrends, Volker; Bell, Thomas; Liebeke, Manuel; Cordes-Blauert, Anne; Ashraf, Syedah N.; Nair, Chandrika Goh; Zlosnik, James E. A.; Williams, Huw D.; Bundy, Jacob G.

doi:10.1074/jbc.m112.442814

Cited by 43 publications

(25 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation is consistent with a previous finding that the number of P. aeruginosa persister cells increases in response to QS and virulence factors (6). Additionally, it has been reported that the loss of rpoN through the deregulation of glucose metabolism increases gluconate production, which correlates with reduced susceptibility to several antibiotics (56).…”

Section: Discussionsupporting

confidence: 82%

RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE

Viducic

Murakami

Amoh

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

bThe ability of Pseudomonas aeruginosa to rapidly modulate its response to antibiotic stress and persist in the presence of antibiotics is closely associated with the process of cell-to-cell signaling. The alternative sigma factor RpoN ( 54 ) is involved in the regulation of quorum sensing (QS) and plays an important role in the survival of stationary-phase cells in the presence of carbapenems. Here, we demonstrate that a ⌬rpoN mutant grown in nutrient-rich medium has increased expression of pqsA, pqsH, and pqsR throughout growth, resulting in the increased production of the Pseudomonas quinolone signal (PQS). The link between pqsA and its role in carbapenem tolerance was studied using a ⌬rpoN ⌬pqsA mutant, in which the carbapenem-tolerant phenotype of the ⌬rpoN mutant was abolished. In addition, we demonstrate that another mechanism leading to carbapenem tolerance in the ⌬rpoN mutant is mediated through pqsE. Exogenously supplied PQS abolished the biapenem-sensitive phenotype of the ⌬rpoN ⌬pqsA mutant, and overexpression of pqsE failed to alter the susceptibility of the ⌬rpoN ⌬pqsA mutant to biapenem. The mutations in the ⌬rpoN ⌬rhlR mutant and the ⌬rpoN ⌬pqsH mutant led to susceptibility to biapenem. Comparison of the changes in the expression of the genes involved in QS in wild-type PAO1 with their expression in the ⌬rpoN mutant and the ⌬rpoN mutant-derived strains demonstrated the regulatory effect of RpoN on the transcript levels of rhlR, vqsR, and rpoS. The findings of this study demonstrate that RpoN negatively regulates the expression of PQS in nutrient-rich medium and provide evidence that RpoN interacts with pqsA, pqsE, pqsH, and rhlR in response to antibiotic stress.

show abstract

Section: Discussionsupporting

confidence: 82%

RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE

Viducic

Murakami

Amoh

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…This adaptation has been termed “metabolic adaptation” [24, 25]. The metabolic changes provide a mechanism to diminish oxidant production and to enhance the production of NADPH, which promotes the formation of antioxidants, as opposed to NADH, which is prooxidant.…”

Section: Biofilms and Oxidant Stressmentioning

confidence: 99%

Pseudomonas aeruginosa and Klebsiella pneumoniae Adaptation to Innate Immune Clearance Mechanisms in the Lung

Riquelme¹,

Ahn²,

Prince³

2018

J Innate Immun

View full text Add to dashboard Cite

Many different species of gram-negative bacteria are associated with infection in the lung, causing exacerbations of chronic obstructive pulmonary disease, cystic fibrosis (CF), and ventilator-associated pneumonias. These airway pathogens must adapt to common host clearance mechanisms that include killing by antimicrobial peptides, antibiotics, oxidative stress, and phagocytosis by leukocytes. Bacterial adaptation to the host is often evident phenotypically, with increased extracellular polysaccharide production characteristic of some biofilm-associated organisms. Given the relatively limited repertoire of bacterial strategies to elude airway defenses, it seems likely that organisms sharing the same ecological niche might also share common strategies to persistently infect the lung. In this review, we will highlight some of the major factors responsible for the adaptation of Pseudomonas aeruginosa to the lung, addressing how growth in biofilms enables persistent infection, relevant to, but not limited to, the pathogenesis of infection in CF. In contrast, we will discuss how carbapenem-resistant Klebsiella pneumoniae evade immune clearance, an organism often associated with ventilator-associated pneumonia and health-care-acquired pneumonias, but not a typical pathogen in CF.

show abstract

“…The detected resonances in NMR spectra were assigned by means of the well established NMR Suite Professional version 7.1 (Chenomx, Alberta, Canada)2627, 2D homonuclear NMR experiments and most reliable online NMR databases based on experimentally acquired spectra: the online Human Metabolome Database28 and Biological Magnetic Resonance Data Bank29 (see Methods for details).…”

Section: Resultsmentioning

confidence: 99%

Molecular degradation of ancient documents revealed by 1H HR-MAS NMR spectroscopy

Corsaro

Mallamace

Łojewska

et al. 2013

Sci Rep

View full text Add to dashboard Cite

For centuries mankind has stored its knowledge on paper, a remarkable biomaterial made of natural cellulose fibers. However, spontaneous cellulose degradation phenomena weaken and discolorate paper over time. The detailed knowledge of products arising from cellulose degradation is essential in understanding deterioration pathways and in improving durability of cultural heritage. In this study, for the first time, products of cellulose degradation were individually detected in solid paper samples by means of an extremely powerful proton HR-MAS NMR set-up, in combination to a wise use of both ancient and, as reference, artificially aged paper samples. Carboxylic acids, in addition to more complex dicarboxylic and hydroxy-carboxylic acids, were found in all samples studied. Since these products can catalyze further degradation, their knowledge is fundamental to improve conservation strategies of historical documents. Furthermore, the identification of compounds used in ancient production techniques, also suggests for artifacts dating, authentication and provenance.

show abstract

Metabolite Profiling to Characterize Disease-related Bacteria

Cited by 43 publications

References 67 publications

RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE

RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE

<b><i>Pseudomonas aeruginosa</i></b> and <b><i>Klebsiella</i></b> <b><i>pneumoniae</i></b> Adaptation to Innate Immune Clearance Mechanisms in the Lung

Molecular degradation of ancient documents revealed by 1H HR-MAS NMR spectroscopy

Contact Info

Product

Resources

About