Metabolites of 3H-Oestradiol-17β in the Cerebrospinal Fluid of the Rhesus Monkey

Tc, Kumar; Gh, Thomas

doi:10.1038/219628a0

Cited by 17 publications

(3 citation statements)

References 7 publications

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“…Among other substances, cortisol is transferred rapidly from CSF to the anterior pituitary gland (Knigge & Scott, 1970). A similar arrangement of paraventricular receptors for CSF oestradiol has been described (Kumar & Thomas, 1968). Other hormones which are present in CSF include adrenocorticotrophic hormone (ACTH) (Allen et al, 1974), antidiuretic hormone (ADH) (Vorherr et al, 1968), melatonin (Anton-Tay & Wurtman, 1969), growth hormone (Linfoot et al, 1970), thyroxine (Hansen & Siersbaek-Nielsen, 1969), insulin (Hunzicker & Hazelwood, 1970), progesterone (Lurie & Weiss, 1967), testosterone (David & Kumar, 1974), and thyrotrophin-releasing hormone (TRH) (Oliver et al, 1974).…”

Section: Discussionmentioning

confidence: 71%

Cerebrospinal fluid and plasma free cortisol concentrations in depression

1976

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SynopsisCerebrospinal fluid (CSF) cortisol levels were examined in a total group of 65 patients. Those who were not depressed (ND), and those suffering from depressive neuroses (DN) had marginally elevated values. Patients with unipolar depression (UD) and bipolar depression (BD) had levels twice as high as the ND and DN patients. Psychotic UD and BD patients had the highest values, three to four times as high as the ND and DN subjects. A significant reduction of CSF cortisol levels was observed following treatment and recovery. Manic patients had moderately elevated CSF cortisol values. The CSF results were in good agreement with plasma total cortisol levels and with urinary free cortisol excretion. Age and sex effects were not responsible for the observed differences; similar results were found in patient subgroups studied in Australia and in the United States. Preliminary equilibrium dialysis data are presented for plasma and CSF cortisol binding. CSF cortisol was 20% bound and 80% free. Plasma free cortisol levels were in good agreement with CSF free cortisol values. Depressed patients have increased tissue and central nervous system (CNS) exposure to free, physiologically active glucocorticoids. The appearance of severe depressive symptoms which manifest a diurnal rhythm may be determined in part by excessive CNS exposure to glucocorticoids.

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Section: Discussionmentioning

confidence: 71%

Cerebrospinal fluid and plasma free cortisol concentrations in depression

1976

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“…CSF samples from dogs injected with oestrogen preparations, produced a cornification of the vaginal epithelium when injected into mice (Whitehead & Huddleston, 1931), indicating that oestrogen diffuses into the CSF. Injection of tritiated oestradiol-17j3 into ovariectomized rhesus monkeys was followed by the appearance of oestrone and oestradiol in CSF (Kumar & Thomas, 1968). Progesterone has been identified in CSF samples from two out of four pregnant patients Ependymal formations in white represent transporting ependyma, i.e.…”

Section: Other Hormonesmentioning

confidence: 99%

The Cerebrospinal Fluid as a Pathway in Neuroendocrine Integration

Rodríguez¹

1976

Journal of Endocrinology

190

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“…Several findings such as the rapid entry into the CSF of gonadal steroids administered systemically [Anand Kumar and Thomas, 1968;Anand Kumar et al, 1974;Marynick et al, 1976Marynick et al, , 1977, the autoradiographic localization of tritiated estradiol on the luminal surface of the ependyma [Anand Kumar and Knowles, 1967] and the occurrence of morphological changes, related to variations in circulating levels of gonadal hormones, also observed on the luminal surface of the tanycyte ependyma [Knowles and Anand Kumar, 1969] which is bathed by the CSF, have all given reason to believe that the CSF also constitutes a humoral vehicle for the transport of gonadal hormones to the brain [Anand Kumar, 1973]. It would therefore seem that studies on the association of steroid hormones with the CSF proteins will contribute to the understanding of the endocrine milieu of the brain and also the interactions between steroid hormones and brain tissue.…”

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confidence: 99%

Association of Tritium-Labelled Estradiol, Progesterone and 17<i>α</i>-Hydroxyprogesterone with the Proteins of the Serum and Cerebrospinal Fluid in the Rhesus Monkey

Umberkoman-Wiita¹,

Kumar²

1979

Neuroendocrinology

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The electrophoretic profiles of the proteins in the serum and cerebrospinal fluid (CSF) of the rhesus monkey were studied by polyacrylamide gel electrophoresis. The association of tritium labelled estradiol (³H-E), progesterone (³H-P) and l7α-hydroxyprogesterone (³H-170HP) with these proteins was studied under in vitro and in vivo conditions. The CSF was found to contain, besides albumin, at least 4 globulins with similar electrophoretic mobilities as those found in the serum. The electrophoretic mobilities of these globulins were Raib 0.4, 0.6, 0.8 and 0.9. The electrophoretic profiles of the monkey serum and CSF proteins were found to be similar to those described for human serum and CSF. ³H-E and ³H-P were respectively associated with serum globulins of Raib 0.4 and 0.8 both in the in vitro and in vivo studies. The respective Raib of these 2 globulins were similar to the testosterone-estradiol binding globulin and cortisol binding globulin in human serum. ³H-170HP was found to be associated with several globulins under both in vitro and in vivo conditions. In marked contrast to the in vitro studies, radioactive products were found to be associated with albumin in vivo. It is presumed that these products may be steroidal conjugates derived from the systemic metabolism of the administered steroids. No radioactivity was associated with the CSF protein after in vitro incubation with any of the three ³H-steroids. In the in vivo studies, however, radioactivity was associated with such of the CSF proteins that were similar to those found in the serum and with which the administered steroids were associated.

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Metabolites of 3H-Oestradiol-17β in the Cerebrospinal Fluid of the Rhesus Monkey

Cited by 17 publications

References 7 publications

Cerebrospinal fluid and plasma free cortisol concentrations in depression

Cerebrospinal fluid and plasma free cortisol concentrations in depression

The Cerebrospinal Fluid as a Pathway in Neuroendocrine Integration

Association of Tritium-Labelled Estradiol, Progesterone and 17<i>α</i>-Hydroxyprogesterone with the Proteins of the Serum and Cerebrospinal Fluid in the Rhesus Monkey

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