Metabolites of nitric oxide as a marker cardiometabolic in blacks

Maurer, Patricia; Bruno, Jamila Benvegnú; Güllich, Angélica Aparecida da Costa; Oliveira, Patrícia Martinez; Pilar, Bruna Cocco; Coelho, Ritiéle Pinto; Nunes, Vinícius Tejada; Manfredini, Vanusa; Piccoli, Jacqueline da Costa Escobar

doi:10.1186/1758-5996-7-s1-a157

Cited by 3 publications

(1 citation statement)

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“…Serum nitric oxide concentrations were indirectly determined according to the protocol described by Maurer et al (2015). Deproteinization process was performed by adding 20 μL of 30% zinc sulphate (diluted in 1N hydrochloric acid) to 400 μL of serum.…”

Section: Analysis Of Serum Nitric Oxide (No)mentioning

confidence: 99%

Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect

Filho

Brito

Faustino

et al. 2021

Reproduction and Fertility

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Effects of conservative treatment on uterine blood flow and morphometric findings are still unknown in bitches. Thus, this study aimed to compare uterine changes of pyometra bitches subjected to distinct modes of treatment. Pyometra bitches were assigned to: OHE (ovariohysterectomy immediately after diagnosis), Aglepristone (days 1, 2 and 8) and Associative (aglepristone treatment coupled with cloprostenol for 7 days) groups. After 9 days, bitches were ovariohysterectomized. Before surgery, uterine area was measured ultrasonographically, and uterine artery Doppler velocimetry analyzed blood flow velocity and indexes. Uterine horns were classified according to resistance index (RI) as more compromised and less compromised. Endometrial vasculature was quantitatively evaluated by color flow Doppler. Blood samples were collected to determine nitric oxide (NO) concentrations. Histological uterine structures were quantified by stereology and VEGF-A (vascular endothelial growth factor) and eNOS (endothelial nitric oxide synthase) immunohistochemically analyzed. Aglepristone and Associative groups had lower uterine area and vascularization, and higher blood flow velocity and indexes compared to OHE group. Less compromised horn of Associative group had higher blood flow velocity compared to OHE group. Aglepristone group presented lower inflammatory infiltrate and larger uterine stroma. Associative group had lower volume density and absolute surface of endometrial cysts and lower VEGF-A expression for glandular epithelium and stromal cells. Blood NO and e-NOS immunostaining were not different among groups. In conclusion, association between aglepristone and prostaglandin is more effective in decreasing uterine vascularization and modulating uterine blood flow. Moreover, associative therapy promotes marked morphological changes.

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Section: Analysis Of Serum Nitric Oxide (No)mentioning

confidence: 99%

Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect

Filho

Brito

Faustino

et al. 2021

Reproduction and Fertility

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In Situ Uterine Artery Prostaglandin E₂ and Nitric Oxide in Open‐Cervix Pyometra and Medically Treated Bitches

Faustino,

da Rosa Filho,

Francischini

et al. 2024

Vet Pharm & Therapeutics

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Uterine vascular alterations take place in pyometra bitches speculatively influenced by prostaglandin and nitric oxide pathways. However, no causative effect of nitric oxide on endometrial vascularization was proved elsewhere for medically treated pyometra bitches. This study aimed to identify the main in situ uterine artery vasodilation pathway in pyometra bitches medically treated with antigestagen solely or coupled with prostaglandin. Pyometra bitches were enrolled into groups: Ovariohysterectomy at diagnosis (Control‐OHE; n = 7), Antigestagen (10 mg/kg aglepristone on Days 1, 2, and 8 after diagnosis; n = 5), and Antigestagen + luteolytic (aglepristone plus 1 μg/kg of cloprostenol from Days 1–7; n = 5). Treated bitches were ovariohysterectomized after 8 days of treatment. Uterine artery fragments from all bitches were collected for tissue nitric oxide and prostaglandin E2 assays. Control‐OHE group had lower uterine artery concentration of nitric oxide compared to treated bitches (Antigestagen and Antigestagen + luteolytic groups). No significant difference was verified between the medical treated groups. Uterine artery concentration of prostaglandin E2 was not different between control and treated bitches, as well as between both treated groups. In conclusion, nitric oxide and prostaglandin E2 are not directly involved in vascular modulation of the uterine artery, albeit pyometra medical treatment influences nitric oxide concentration in the uterine artery.

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Clinical Changes and Uterine Hemodynamic in Pyometra Medically Treated Bitches

Filho

Brito

Faustino

et al. 2020

Animals

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Cystic endometrial hyperplasia–pyometra complex is a canine endometrial disorder, considered the most common and important among uterine diseases. The treatment of choice is ovariohysterectomy, but medical treatments have become an alternative. However, no studies have been performed in order to evaluate uterine hemodynamic changes during medical treatment for pyometra bitches. Therefore, the aim of this study was to compare the effectiveness of two medical protocols by means of clinical (heart rate, respiratory rate and body temperature), medullar response (complete blood count (CBC)) and uterine hemodynamic evaluation in pyometra bitches. For such purpose, 10 bitches with pyometra were assigned to two groups: Aglepristone Group (n = 5) and Associative Group (aglepristone + prostaglandin; n = 5). The Associative Group had altered respiratory rate and decreased body temperature. The Aglepristone Group had decreased plasma protein, however, leukocyte count reduced over time for both therapeutic protocols. Uterine area and vascularization score decreased throughout treatment. There was a significant reduction in uterine perimeter, area and vascularization score. The Associative Group had lower final diastolic velocity and higher systole:diastole ratio (S/D), pulsatility index (PI) and resistance index (RI). In conclusion, both medical treatments are effective in reversing clinical and CBC changes of pyometra, especially aglepristone, however they cannot fully restore local uterine changes in a short-term evaluation. Conversely, associative therapy was more effective in decreasing uterine vascularization and modulating uterine blood flow.

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Metabolites of nitric oxide as a marker cardiometabolic in blacks

Cited by 3 publications

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Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect

Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect

In Situ Uterine Artery Prostaglandin E₂ and Nitric Oxide in Open‐Cervix Pyometra and Medically Treated Bitches

Clinical Changes and Uterine Hemodynamic in Pyometra Medically Treated Bitches

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Metabolites of nitric oxide as a marker cardiometabolic in blacks

Cited by 3 publications

References 0 publications

Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect

Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect

In Situ Uterine Artery Prostaglandin E2 and Nitric Oxide in Open‐Cervix Pyometra and Medically Treated Bitches

Clinical Changes and Uterine Hemodynamic in Pyometra Medically Treated Bitches

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Product

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In Situ Uterine Artery Prostaglandin E₂ and Nitric Oxide in Open‐Cervix Pyometra and Medically Treated Bitches