Metabolome and microbiome analyses of the anti-fatigue mechanism of<i>Acanthopanax senticosus</i>leaves

Shi, Chao; Liang, Zehua; Li, Ting; Hao, Qi; Xiang, Hongyu; Xie, Qiuhong

doi:10.1039/d3fo05311c

Food Funct.

2024

DOI: 10.1039/d3fo05311c

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Metabolome and microbiome analyses of the anti-fatigue mechanism ofAcanthopanax senticosusleaves

Chao Shi,

Zehua Liang,

Ting Li

et al.

Abstract: Acanthopanax senticosus leaves, widely used as a vegetable and tea, have been proven to be beneficial for neurological disorders. At present, its anti-fatigue effect remains to be established. In this...

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Optimization of Liquid Fermentation of Acanthopanax senticosus Leaves and Its Non-Targeted Metabolomics Analysis

Zhang,

Wang,

Xue

et al. 2024

Molecules

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To enhance the nutritional value of Acanthopanax senticosus leaves (AL), a fermentation process was conducted using a probiotic Bacillus mixture, and the changes in chemical constituents and biological activities before and after fermentation were compared. A response surface methodology was employed to optimize the liquid fermentation conditions of AL based on their influence on polyphenol content. Non-targeted metabolomics analysis was performed using LC-MS/MS to reveal the differing profiles of compounds before and after fermentation. The results indicated that Bacillus subtilis LK and Bacillus amyloliquefaciens M2 significantly influenced polyphenol content during fermentation. The optimal fermentation conditions were determined to be a fermentation time of 54 h, a temperature of 39.6 °C, and an inoculum size of 2.5% (v/v). In comparison to unfermented AL, the total polyphenol and flavonoid contents, as well as the free radical scavenging capacities measured by DPPH and ABTS assays, and the activities of β-glucosidase and endo-glucanase, were significantly increased. The non-targeted metabolomics analysis identified 1348 metabolites, of which 829 were classified as differential metabolites. A correlation analysis between the differential metabolites of polyphenols, flavonoids, and antioxidant activity revealed that 13 differential metabolites were positively correlated with antioxidant activity. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis of the differential metabolites identified 82 pathways, with two of the top 25 metabolic pathways related to flavonoids. This study explores the potential for enhancing the active ingredients and biological effects of AL through probiotic fermentation using Bacillus strains.

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Optimization of Liquid Fermentation of Acanthopanax senticosus Leaves and Its Non-Targeted Metabolomics Analysis

Zhang,

Wang,

Xue

et al. 2024

Molecules

View full text Add to dashboard Cite

show abstract

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Metabolome and microbiome analyses of the anti-fatigue mechanism ofAcanthopanax senticosusleaves

Abstract: Acanthopanax senticosus leaves, widely used as a vegetable and tea, have been proven to be beneficial for neurological disorders. At present, its anti-fatigue effect remains to be established. In this...

Cited by 1 publication

References 74 publications

Optimization of Liquid Fermentation of Acanthopanax senticosus Leaves and Its Non-Targeted Metabolomics Analysis

Optimization of Liquid Fermentation of Acanthopanax senticosus Leaves and Its Non-Targeted Metabolomics Analysis

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