Metabolome and transcriptome analysis of postharvest peach fruit in response to fungal pathogen Monilinia fructicola infection

Chen, Cheng; Yan, Chengying; Qi, Cheng-Tian; Zhao, Xin; Liu, Lingxiao; Guo, Yaping; Leng, Peng; Sun, Jie; Ahmtijiang,; Li, Jun; Liu, Yun-Guo

doi:10.1016/j.lwt.2022.114301

Cited by 14 publications

(7 citation statements)

References 58 publications

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“…Thus, although an abundant number of pathogenicity-related genes were significantly upregulated in response to M. fructicola infection, mostly at 24 and 48 HAI, it was insufficient to limit fungal expansion. Our results are consistent with several studies where such genes were induced in peach fruit upon infection with the Monilinia species, indicating their involvement in brown rot tolerance [ 51 , 52 ].…”

Section: Discussionsupporting

confidence: 93%

“…Mainly at a late time point, DEGs associated with hampering the pathogen growth including XTH and CesA genes, which are related to cell-wall thickening and cellulose synthesis were upregulated for the MF-CT group. Such genes were also significantly induced at 48 HAI upon M. fructicola infection [ 51 ]. EXT genes involved in cell-wall reinforcement were constitutively upregulated across the three time points.…”

Section: Discussionmentioning

confidence: 99%

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Global Transcriptome Analysis of the Peach (Prunus persica) in the Interaction System of Fruit–Chitosan–Monilinia fructicola

Tsalgatidou,

Boutsika,

Papageorgiou

et al. 2024

Plants

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The peach (Prunus persica L.) is one of the most important stone-fruit crops worldwide. Nevertheless, successful peach fruit production is seriously reduced by losses due to Monilinia fructicola the causal agent of brown rot. Chitosan has a broad spectrum of antimicrobial properties and may also act as an elicitor that activate defense responses in plants. As little is known about the elicitation potential of chitosan in peach fruits and its impact at their transcriptional-level profiles, the aim of this study was to uncover using RNA-seq the induced responses regulated by the action of chitosan in fruit–chitosan–M. fructicola interaction. Samples were obtained from fruits treated with chitosan or inoculated with M. fructicola, as well from fruits pre-treated with chitosan and thereafter inoculated with the fungus. Chitosan was found to delay the postharvest decay of fruits, and expression profiles showed that its defense-priming effects were mainly evident after the pathogen challenge, driven particularly by modulations of differentially expressed genes (DEGs) related to cell-wall modifications, pathogen perception, and signal transduction, preventing the spread of fungus. In contrast, as the compatible interaction of fruits with M. fructicola was challenged, a shift towards defense responses was triggered with a delay, which was insufficient to limit fungal expansion, whereas DEGs involved in particular processes have facilitated early pathogen colonization. Physiological indicators of peach fruits were also measured. Additionally, expression profiles of particular M. fructicola genes highlight the direct antimicrobial activity of chitosan against the fungus. Overall, the results clarify the possible mechanisms of chitosan-mediated tolerance to M. fructicola and set new foundations for the potential employment of chitosan in the control of brown rot in peaches.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Global Transcriptome Analysis of the Peach (Prunus persica) in the Interaction System of Fruit–Chitosan–Monilinia fructicola

Tsalgatidou,

Boutsika,

Papageorgiou

et al. 2024

Plants

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“…Kyoto Encyclopedia of Genes and Genomes pathway analysis can further understand the biological functions and interactions of related genes ( Cheng et al, 2023 ). The results showed ( Figure 3 ) that HT vs. CK differential genes were significantly enriched in “Galactose metabolism,” “alpha-Linolenic acid metabolism,” “Photosynthesis,” and “Glucosinolate biosynthesis” pathways.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years multi-omics co-analysis methods are often used in studies on plant and pathogen infections, e.g., accumulation of total flavonoid content is induced in wheat resistance to powdery mildew ( Xu et al, 2023 ); induction of energy metabolism and nitrogen mobilization in Phaseolus vulgaris L. roots infected with Fusarium oxysporum f. sp. phaseoli ( Chen et al, 2019 ); after picking peach fruits were infested with Monilinia fructicola for 12 h, jasmonic acid responded to the infestation as a signaling molecule, and salicylic acid responded to the infestation as a signaling molecule 48 h after infestation ( Cheng et al, 2023 ). Currently, research on multiple fungal diseases of mulberry is dominated by the isolation and identification of pathogenic fungi and their control ( Maji et al, 2005 ; ; Ghosh et al, 2016 ; Bao et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Integrating transcriptomics and metabolomics to analyze the defense response of Morus notabilis to mulberry ring rot disease

Qian,

Deng,

Mureed

et al. 2024

Front. Microbiol.

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IntroductionThe mulberry industry has thrived in China for millennia, offering significant ecological and economic benefits. However, the prevalence of mulberry ring rot disease poses a serious threat to the quality and yield of mulberry leaves.MethodsIn this study, we employed a combination of transcriptomic and metabolomic analyses to elucidate the changes occurring at the transcriptional and metabolic levels in Morus notabilis in response to this disease infestation. Key metabolites identified were further validated through in vitro inhibition experiments.ResultsThe findings revealed significant enrichment in Kyoto Encyclopedia of Genes and Genomes pathways, particularly those related to flavonoid biosynthesis. Notably, naringenin, kaempferol, and quercetin emerged as pivotal players in M. notabilis’ defense mechanism against this disease pathogen. The upregulation of synthase genes, including chalcone synthase, flavanone-3-hydroxylase, and flavonol synthase, suggested their crucial roles as structural genes in this process. In vitro inhibition experiments demonstrated that kaempferol and quercetin exhibited broad inhibitory properties, while salicylic acid and methyl jasmonate demonstrated efficient inhibitory effects.DiscussionThis study underscores the significance of the flavonoid biosynthesis pathway in M. notabilis’ defense response against mulberry ring rot disease, offering a theoretical foundation for disease control measures.

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“…Examples of these interaction studies include the phytopathogens Monilinia fructicola, Botrytis cinerea, Penicillium expansum, Penicillium digitatum, and their respective hosts: peach, blueberry, apple, kiwi, and citrus, respectively. [60][61][62][63][64] 3 Postharvest diseases in citrus and metabolomics approaches…”

Section: Metabolomics Approaches To Understand Host-pathogen Interact...mentioning

confidence: 99%

Metabolomics approach to understand molecular mechanisms involved in fungal pathogen–citrus pathosystems

Silva,

Dantas,

Barbosa

et al. 2024

Mol. Omics

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Metabolome and transcriptome analysis of postharvest peach fruit in response to fungal pathogen Monilinia fructicola infection

Cited by 14 publications

References 58 publications

Global Transcriptome Analysis of the Peach (Prunus persica) in the Interaction System of Fruit–Chitosan–Monilinia fructicola

Global Transcriptome Analysis of the Peach (Prunus persica) in the Interaction System of Fruit–Chitosan–Monilinia fructicola

Integrating transcriptomics and metabolomics to analyze the defense response of Morus notabilis to mulberry ring rot disease

Metabolomics approach to understand molecular mechanisms involved in fungal pathogen–citrus pathosystems

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