Metabolome of Mammary Tumors Differs from Normal Mammary Glands But Is Not Altered by Time-restricted Feeding Under Obesogenic Conditions

Yan, Lin; Sundaram, Shanthy; Rust, Bret; Picklo, Matthew J.; Bukowski, Michael R.

doi:10.21873/anticanres.14358

Cited by 4 publications

(4 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cholesterol is essential for membrane biogenesis and a precursor for steroid hormone synthesis. Cholesterol is elevated in mammary tumors in MMTV-PyMT mice fed an HFD ( 55 ). Hypercholesterolemia promotes mammary tumorigenesis in MMTV-PyMT mice ( 56 , 57 ).…”

Section: Discussionmentioning

confidence: 99%

Mammary Tumorigenesis and Metabolome in Male Adipose Specific Monocyte Chemotactic Protein-1 Deficient MMTV-PyMT Mice Fed a High-Fat Diet

et al. 2021

Self Cite

View full text Add to dashboard Cite

Male breast cancer, while uncommon, is a highly malignant disease. Monocyte chemotactic protein-1 (MCP-1) is an adipokine; its concentration in adipose tissue is elevated in obesity. This study tested the hypothesis that adipose-derived MCP-1 contributes to male breast cancer. In a 2x2 design, male MMTV-PyMT mice with or without adipose-specific Mcp-1 knockout [designated as Mcp-1-/- or wild-type (WT)] were fed the AIN93G standard diet or a high-fat diet (HFD) for 25 weeks. Mcp-1-/- mice had lower adipose Mcp-1 expression than WT mice. Adipose Mcp-1 deficiency reduced plasma concentrations of MCP-1 in mice fed the HFD compared to their WT counterparts. Mcp-1-/- mice had a longer tumor latency (25.2 weeks vs. 18.0 weeks) and lower tumor incidence (19% vs. 56%), tumor progression (2317% vs. 4792%), and tumor weight (0.23 g vs. 0.64 g) than WT mice. Plasma metabolomics analysis identified 56 metabolites that differed among the four dietary groups, including 22 differed between Mcp-1-/- and WT mice. Pathway and network analyses along with discriminant analysis showed that pathways of amino acid and carbohydrate metabolisms are the most disturbed in MMTV-PyMT mice. In conclusion, adipose-derived MCP-1 contributes to mammary tumorigenesis in male MMTV-PyMT. The potential involvement of adipose-derived MCP-1 in metabolomics warrants further investigation on its role in causal relationships between cancer metabolism and mammary tumorigenesis in this male MMTV-PyMT model.

show abstract

Section: Discussionmentioning

confidence: 99%

Mammary Tumorigenesis and Metabolome in Male Adipose Specific Monocyte Chemotactic Protein-1 Deficient MMTV-PyMT Mice Fed a High-Fat Diet

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Laboratory rodents are nocturnal animals. They are active in the dark phase of the day (ZT [12][13][14][15][16][17][18][19][20][21][22][23][24]. In a recent transcriptome analysis of liver samples from these pubertal mice, we found that the HFD-fed mice, compared to the AIN93G-fed controls, had greater numbers of differentially expressed genes and KEGG metabolic signaling pathways in the dark phase (particularly ZT [16][17][18][19][20] and fewer numbers in the light phase.…”

Section: Discussionmentioning

confidence: 93%

“…Feeding weanling mice a high-fat diet increases body fat mass, 13 , 14 disrupts the pattern of metabolism (eg, respiratory exchange ratio), 14 and changes plasma concentrations of cytokines that are involved in metabolism (eg, leptin and adiponectin). 13 , 14 Furthermore, the high-fat diet alters metabolic profile of mammary tumors 15 and enhances mammary tumorigenesis 16 in MMTV-PyMT mice. However, there is a lack of knowledge of the impact of dietary fat on pubertal mammary metabolism.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Alterations in Mammary Glands from Pubertal Mice Fed a High-Fat Diet

Yan

Rust

Sundaram

et al. 2023

Nutr Metab Insights

Self Cite

View full text Add to dashboard Cite

Dietary malpractice is a risk factor for obesity. This study tested the hypothesis that consumption of a high-fat diet alters mammary metabolome in pubertal mice. We performed untargeted metabolomic analysis of primary metabolism on mammary glands from pubertal mice fed the AIN93G standard diet or a high-fat diet (HFD) for 3 weeks. We identified 97 metabolites for statistical comparisons. The HFD altered the amino acid metabolism considerably. This included elevated expression of branched-chain amino acids, non-essential amino acids (aspartic acid and glutamic acid), and methionine sulfoxide (oxidized methionine) and an alteration in the aminoacyl-tRNA biosynthesis pathway. Furthermore, elevations of fumaric acid and malic acid (both are citrate cycle intermediates) and glyceric acid (its phosphate derivatives are intermediates of glycolysis) in HFD-fed mice suggest an acceleration of both citrate cycle and glycolysis. Lower expression of glycerol, oleic acid, and palmitoleic acid, as well as decreased mammary expression of genes encoding lipid metabolism ( Acaca, Fads1, Fasn, Scd1, and Srebf1) in HFD-fed mice indicate an attenuated lipid metabolism in the presence of adequate dietary fat. In conclusion, consumption of the HFD for 3 weeks alters metabolic profile of pubertal mammary glands. This alteration may affect mammary development and growth in pubertal mice.

show abstract

“…They also observed that TRF decreased the phosphorylation of AKT (also known as PKB, protein kinase B) in tumor tissues, supporting the idea that TRF may be related to autophagy. Yan et al [49–51] also demonstrated that TRF could attenuate the spontaneous metastasis of Lewis lung cancer and reduce the expression of oncogenic genes through a series of animal experiments.…”

Section: Potential Antitumor Effects Of Trementioning

confidence: 99%

Potential preventive effects of time-restricted eating on cancer

Fang,

Yan,

Liao

2023

J Nutr Oncol

View full text Add to dashboard Cite

Intermittent fasting is a novel dietary intervention that has been demonstrated to improve the efficacy of cancer therapy and reduce complications. Time-restricted eating is a specific form of intermittent fasting. It usually restricts only the daily eating window and not the amount of calories consumed. In addition to providing a means for weight management and improving metabolism like other intermittent fasting methods, time-restricted eating also synchronizes circadian rhythms and affects autophagy levels, which has potential antiaging effects. Some studies have shown that time-restricted eating can prevent tumorigenesis and slow tumor progression. Moreover, because of its simplicity and mild adverse effects, time-restricted eating is safe and has a high rate of compliance. It may be used an adjuvant therapy and may be part of a healthy lifestyle suitable for long term by cancer patients or those at high risk of developing specific cancers. This article expounds on the potential impact of time-restricted eating on the prevention and treatment of cancer, as well as the potential mechanism(s) of action. There are 3 main aspects considered to be impacted by time-restricted eating: improving obesity and metabolic disorders, synchronizing the circadian rhythm, and upregulating autophagy. We herein present the current research progress related to the effects of time-restricted eating on malignant tumors. The purpose of this review is to provide a theoretical basis and direction for future research to explore the potential of time-restricted eating as a nutritional intervention to prevent the occurrence of cancer and as a treatment or adjuvant therapy for cancer. We believe that this approach may be conducive to improving the nutritional status of cancer patients and improving their prognosis and quality of life.

show abstract

Metabolome of Mammary Tumors Differs from Normal Mammary Glands But Is Not Altered by Time-restricted Feeding Under Obesogenic Conditions

Cited by 4 publications

References 40 publications

Mammary Tumorigenesis and Metabolome in Male Adipose Specific Monocyte Chemotactic Protein-1 Deficient MMTV-PyMT Mice Fed a High-Fat Diet

Mammary Tumorigenesis and Metabolome in Male Adipose Specific Monocyte Chemotactic Protein-1 Deficient MMTV-PyMT Mice Fed a High-Fat Diet

Metabolomic Alterations in Mammary Glands from Pubertal Mice Fed a High-Fat Diet

Potential preventive effects of time-restricted eating on cancer

Contact Info

Product

Resources

About