Metabolomic analyses reveal the antibacterial properties of a novel antimicrobial peptide MOp3 from Moringa oleifera seeds against Staphylococcus aureus and its application in the infecting pasteurized milk

Sun, Aidi; Huang, Zhiyuan; He, Li; Dong, Wenming; Yang, Tian; Huang, Aixiang; Wang, Xuefeng

doi:10.1016/j.foodcont.2023.109779

Cited by 13 publications

(6 citation statements)

References 61 publications

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Section: Resultsmentioning

confidence: 99%

“…These findings confirmed that KTA and KTR had excellent bacteriostatic effect against S. aureus as well as MRSA in pasteurized milk stored at room temperature, suggesting the potential application prospect of these AMPs in the field of food preservation. 38 cells treated with a 4 × MIC of AMPs for 1 h show significant changes. For instance, cellular morphological abnormalities, such as rough and wrinkled surfaces, are manifested in S. aureus cells.…”

Section: Antibacterial Efficiency Of Ampsmentioning

confidence: 99%

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Chickpea-Derived Modified Antimicrobial Peptides KTA and KTR Inactivate Staphylococcus aureus via Disrupting Cell Membrane and Interfering with Peptidoglycan Synthesis

Zhang,

Ma,

Ismail

et al. 2024

J. Agric. Food Chem.

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The widespread bacterial contamination caused by foodborne pathogens has continuously driven the development of advanced and potent food antimicrobial agents. In this study, two novel antimicrobial peptides (AMPs) named KTA and KTR were obtained by modifying a natural AMP, Leg2, from chickpea storage protein legumin hydrolysates. They were further predicted to be stable hydrophobic cationic AMPs of α-helical structure with no hemolytic toxicity by several online servers. Moreover, the AMPs exerted superior antibacterial activity against two representative Staphylococcus aureus strains thanks to the increased hydrophobicity and positive charge, with minimum inhibition concentration value (4.74−7.41 μM) significantly lower than that of Leg2 (>1158.70 μM). Further, this study sought to elucidate the specific antimicrobial mechanism against Gram-positive bacteria. It was found that the electrostatic interactions of the AMPs with peptidoglycan were vital for peptide activity in combating Gram-positive bacteria. Subsequently, the cell membrane of S. aureus cells was irreversibly disrupted by increasing permeability and impairing membrane components, which led to the massive release of intracellular substances and eventual cell death. Overall, this work demonstrated that KTA and KTR were active against Gram-positive bacteria via peptidoglycan targeting and membrane-disruptive mechanisms and paved the way for expanding their application potential to alleviate food contamination.

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Section: Resultsmentioning

confidence: 99%

Section: Antibacterial Efficiency Of Ampsmentioning

confidence: 99%

Chickpea-Derived Modified Antimicrobial Peptides KTA and KTR Inactivate Staphylococcus aureus via Disrupting Cell Membrane and Interfering with Peptidoglycan Synthesis

Zhang,

Ma,

Ismail

et al. 2024

J. Agric. Food Chem.

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“…Therefore, this likely impairs the repair of membrane damage induced by synthetic cruzioseptin. In fact, other researchers have also shown that cationic peptides induce variations in the expression of intracellular molecules involved in membrane structure (Bo et al, 2014;Sun et al, 2023). On the other hand, the TCA cycle is a central metabolic pathway which functions as a point of convergence in the catabolism of sugar, lipids, or amino acids in all aerobic microorganisms (Li et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…In line with this, modern technologies, such as the metabolomics constitute valuable analytical tools to build a comprehensive understanding of biochemical alterations in pathogens after AMP exposure. However, there are few examples of integrative efforts combining different strategies to explore this mechanistic scenario ( Bo et al, 2014 ; Sun et al, 2023 ). Based on this, the aim of this work was to expand the current understanding of the potential applications of CZS-9, CZS12, [K4K15]CZS-1, by examining the activities against S. Typhimurium and RAW 264.7 murine macrophage cells.…”

Section: Introductionmentioning

confidence: 99%

Dual antibacterial mechanism of [K4K15]CZS-1 against Salmonella Typhimurium: a membrane active and intracellular-targeting antimicrobial peptide

Bermúdez-Puga,

Dias,

Freire de Oliveira

et al. 2023

Front. Microbiol.

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Salmonella genus is a leading cause of food-borne infections with strong public health impact and economic ramifications. The development of antimicrobial resistance added complexity to this scenario and turned the antibiotic drug discovery into a highly important challenge. The screening of peptides has served as a successful discovery platform to design new antibiotic candidates. Motivated by this, the antimicrobial and cytotoxic properties of three cruzioseptins against Salmonella Typhimurium and RAW 264.7 murine macrophage cells, respectively, were investigated. [K4K15]CZS-1 was the most potent antimicrobial peptide identified in the screening step with a minimum inhibitory concentration (MIC) of 16 μg/mL (7.26 μM) and moderate cytotoxicity. From a structural point of view, in vitro and in silico techniques evidenced that [K4K15]CZS-1 is a α-helical cationic antimicrobial peptide. In order to capture mechanistic details and fully decipher their antibacterial action, we adopted a multidimensional approach, including spectroscopy, electron microscopy and omics analysis. In general lines, [K4K15]CZS-1 caused membrane damage, intracellular alterations in Salmonella and modulated metabolic pathways, such as the tricarboxylic acid (TCA) cycle, fatty acid biosynthesis, and lipid metabolism. Overall, these findings provide deeper insights into the antibacterial properties and multidimensional mode of action of [K4K15]CZS-1 against Salmonella Typhimurium. In summary, this study represents a first step toward the screening of membrane-acting and intracellular-targeting peptides as potential bio-preservatives to prevent foodborne outbreaks caused by Salmonella.

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“…Besides, while this extract exhibits weaker effectiveness against Pseudomonas aeruginosa and E. coli compared to Bacillus cereus, it demonstrates strong inhibitory effects against Bacillus cereus, Staphylococcus aureus, Mucor species, and Aspergillus species, suggesting potential for treating the related infections caused by these organisms. It also shows inhibitory effects against bacteria such as Staphylococcus aureus [13]and Vibrio cholera [12,14]. The antimicrobial effect of Moringa oleifera seeds is primarily attributed to its active components (to moringine, pterygospermine, and 4-(alpha-L-rhamanosyloxy) benzyl isothiocyanates [15]).…”

Section: Antimicrobial Seeds Activitiesmentioning

confidence: 99%

Natural Medicine: In-Depth Exploration of Moringa oleifera’s Bioactive Compounds and Antimicrobial Effects

Moummou,

Meftah

2024

The Global Burden of Disease and Risk Factors - Understanding and Management

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The antimicrobial capabilities of Moringa oleifera have garnered significant scientific attention due to its rich array of bioactive compounds. This chapter provides a comprehensive examination of the antimicrobial activities exhibited by various components of the Moringa oleifera plant, including seeds, leaves, roots, fruits, and flowers. Notably, Moringa seeds, containing potent 4-(alpha-L-rhamanosyloxy) benzyl isothiocyanates, demonstrate strong antimicrobial effects against a broad spectrum of bacterial strains, including Bacillus cereus and Staphylococcus aureus, as well as fungi. Furthermore, lectins within Moringa seeds interact with bacterial membranes, impeding growth and viability. Moringa leaves exhibit pronounced antimicrobial actions against both Gram-positive and Gram-negative bacteria, facilitated by phenolic compounds that disrupt essential bacterial functions. Similarly, Moringa roots demonstrate antibacterial and antifungal properties, attributed to compounds like N-benzylethyl thioformate, presenting promising alternatives to conventional antibiotics. Additionally, Moringa fruits and flowers display significant antimicrobial efficacy, with bioactive compounds such as phenols and flavonoids demonstrating activity against common pathogens like Candida albicans and Escherichia coli. This in-depth analysis underscores the multifaceted antimicrobial potential of Moringa oleifera, highlighting pathways for further research and the development of novel antimicrobial agents and nutraceuticals.

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Metabolomic analyses reveal the antibacterial properties of a novel antimicrobial peptide MOp3 from Moringa oleifera seeds against Staphylococcus aureus and its application in the infecting pasteurized milk

Cited by 13 publications

References 61 publications

Chickpea-Derived Modified Antimicrobial Peptides KTA and KTR Inactivate Staphylococcus aureus via Disrupting Cell Membrane and Interfering with Peptidoglycan Synthesis

Chickpea-Derived Modified Antimicrobial Peptides KTA and KTR Inactivate Staphylococcus aureus via Disrupting Cell Membrane and Interfering with Peptidoglycan Synthesis

Dual antibacterial mechanism of [K4K15]CZS-1 against Salmonella Typhimurium: a membrane active and intracellular-targeting antimicrobial peptide

Natural Medicine: In-Depth Exploration of Moringa oleifera’s Bioactive Compounds and Antimicrobial Effects

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