Metabolomic analysis of biochemical changes in the plasma and urine of collagen-induced arthritis in rats after treatment with Huang-Lian-Jie-Du-Tang

Zhang, Huawei; Fu, Ping; Ke, Beilei; Wang, Shuping; Li, Min; Lin, Han; Peng, Chengcheng; Zhang, Guoqing; Liu, Runhui

doi:10.1016/j.jep.2014.03.007

Cited by 51 publications

(29 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, higher levels of phenaceturic acid and hippuric acid were found in the urine of rats with AA, consistent with results from the literature (Zhang et al, 2014a), and further implying an association between lipid metabolism dysfunction and RA. In disease states associated with RA, large amounts of oxygen free radicals are produced in the mitochondria, which accelerate fat mobilization, eventually leading to increases in the levels of associated secondary metabolites.…”

Section: Discussionsupporting

confidence: 92%

Urinary metabolite profiling provides potential differentiation to explore the mechanisms of adjuvant‐induced arthritis in rats

Jiang

Liu

Wang

et al. 2016

Biomedical Chromatography

View full text Add to dashboard Cite

To explore the pathogenesis of rheumatoid arthritis (RA) from the perspective of metabolomics, gas chromatography time-of-flight mass spectrometry (GC-TOF/MS) technology was used to observe changes in the metabolic profiles of urine output from rats with adjuvant-induced arthritis (AA). Sprague-Dawley rats were randomly divided into a control group and an experimental group, with eight in each. Rats in the experimental group were induced by intracutaneous innoculation of 0.1 mL Freund's complete adjuvant to right paws. On day 20 after immunization, the metabolic profiles between rat control and experimental groups were compared by combining GC-TOF/MS technology with multivariate statistical approaches, including principal component analysis, partial least squares discriminant analysis and orthogonal projections to latent structures-discriminant analysis. Nine potential biomarkers were identified, including 2,2-dimethylsuccinic acid, tartronic acid, dehydroshikimic acid, hippuric acid, adenine, phenaceturic acid, l-dopa, 1,4-dihydroxy-2-naphthoic acid and melibiose. The findings indicate that the rats with AA are disturbed in metabolism of purine, amino acid, fat and energy. This study also demonstrates that the dysfunction in a range of biosynthetic and catabolic pathways, which leads to increased oxygen free radicals and inflammation, could cause underlying pathogenesis of RA. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Section: Discussionsupporting

confidence: 92%

Urinary metabolite profiling provides potential differentiation to explore the mechanisms of adjuvant‐induced arthritis in rats

Jiang

Liu

Wang

et al. 2016

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…It has previously been shown that lung infections with different microorganisms in mouse models induce unique metabolite patterns in urine and plasma (Lu et al, 2012;Slupsky et al, 2009a;Slupsky et al, 2009b). In recent years, an increasing number of studies have investigated the holistic therapeutic effects of TCM scientifically, via metabolomics (Dai et al, 2010;Jiang et al, 2011;Zhang et al, 2014;Zhao et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

A metabolomics approach to studying the effects of Jinxin oral liquid on RSV-infected mice using UPLC/LTQ-Orbitrap mass spectrometry

Xie

et al. 2015

Journal of Ethnopharmacology

View full text Add to dashboard Cite

“…For unique differential metabolites of cold pattern compared with healthy controls, up-regulated urea, l -proline and citric acid represented three characteristic pathways of cold pattern. Citric acid disturbance can be found in animal models of RA [26], and up-regulated l -proline and urea indicate more protein and collagen breakdown in cold pattern. Other work [27] also shows that the cold pattern patients experience low level of energy metabolism, which leptin was confirmed as a biomarker in cold pattern.…”

Section: Discussionmentioning

confidence: 99%

Differential Amino Acid, Carbohydrate and Lipid Metabolism Perpetuations Involved in a Subtype of Rheumatoid Arthritis with Chinese Medicine Cold Pattern

Guo

Niu

et al. 2016

IJMS

View full text Add to dashboard Cite

Pattern classification is a key approach in Traditional Chinese Medicine (TCM), and it is used to classify the patients for intervention selection accordingly. TCM cold and heat patterns, two main patterns of rheumatoid arthritis (RA) had been explored with systems biology approaches. Different regulations of apoptosis were found to be involved in cold and heat classification in our previous works. For this study, the metabolic profiling of plasma was explored in RA patients with typical TCM cold or heat patterns by integrating liquid chromatography/mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC/MS) platforms in conjunction with the Ingenuity Pathway Analysis (IPA) software. Three main processes of metabolism, including amino acid, carbohydrate and lipid were focused on for function analysis. The results showed that 29 and 19 differential metabolites were found in cold and heat patterns respectively, compared with healthy controls. The perturbation of amino acid metabolism (increased essential amino acids), carbohydrate metabolism (galactose metabolism) and lipid metabolism, were found to be involved in both cold and heat pattern RA. In particular, more metabolic perturbations in protein and collagen breakdown, decreased glycolytic activity and aerobic oxidation, and increased energy utilization associated with RA cold pattern patients. These findings may be useful for obtaining a better understanding of RA pathogenesis and for achieving a better efficacy in RA clinical practice.

show abstract

Metabolomic analysis of biochemical changes in the plasma and urine of collagen-induced arthritis in rats after treatment with Huang-Lian-Jie-Du-Tang

Cited by 51 publications

References 28 publications

Urinary metabolite profiling provides potential differentiation to explore the mechanisms of adjuvant‐induced arthritis in rats

Urinary metabolite profiling provides potential differentiation to explore the mechanisms of adjuvant‐induced arthritis in rats

A metabolomics approach to studying the effects of Jinxin oral liquid on RSV-infected mice using UPLC/LTQ-Orbitrap mass spectrometry

Differential Amino Acid, Carbohydrate and Lipid Metabolism Perpetuations Involved in a Subtype of Rheumatoid Arthritis with Chinese Medicine Cold Pattern

Contact Info

Product

Resources

About