Metabolomic Analysis of Cold Acclimation of Arctic Mesorhizobium sp. Strain N33

Ghobakhlou, Abdollah; Laberge, Serge; Antoun, Hani; Wishart, David S.; Xia, Jianguo; Krishnamurthy, R.; Mandal, Rupasri

doi:10.1371/journal.pone.0084801

Cited by 14 publications

(11 citation statements)

References 55 publications

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“…Mesorhizobium sp. strain N 33 was shown to overexpress myristoleic acid (14:1 ω 11 ) after 1 h of exposure to 4 °C, whereas the strains analyzed in the present study did not synthesize this fatty acid at any of the temperatures applied .…”

Section: Discussioncontrasting

confidence: 54%

Low temperature adaptation and the effects of cryoprotectants on mesorhizobia strains

Wdowiak-Wróbel

Małek

Palusińska-Szysz

2016

J. Basic Microbiol.

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In this study, the tolerance of Mesorhizobium sp. ACMP18, Mesorhizobium sp. USDA3350, and Mesorhizobium temperatum LMG23931 strains, to cold and freezing were investigated. The ability to withstand freezing at -20 °C and -70 °C for 24 months was different among the studied strains and depended on the cryoprotectant used. The survivability of mesorhizobial strains at -20 °C and -70 °C was significantly improved by some cryoprotectans (glycerol and sucrose/peptone). It is worth noting that the greatest resistance to freezing was detected when stress treatments were performed in glycerol as a cryoprotectant. Using PCR analysis, cspA genes were identified in the studied strains. Their nucleotide sequences were most similar to the sequences of the corresponding genes of the Mesorhizobium species. The expression of the cspA gene in the studied bacteria was analyzed using the RT-PCR technique. The fatty acid composition of the mesorhizobia was determined at 5, 10, 15, and 28 °C. It was noticed that growth temperature significantly affected the fatty acid composition and the amounts of unsaturated fatty acids, especially that of cis-vaccenic acid (18:1ɷ(11)), increased markedly in bacterial cells cultivated at 5, 10, and 15 °C.

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Section: Discussioncontrasting

confidence: 54%

Low temperature adaptation and the effects of cryoprotectants on mesorhizobia strains

Wdowiak-Wróbel

Małek

Palusińska-Szysz

2016

J. Basic Microbiol.

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“…Ventricular samples from week 5 of tamoxifen treatment were analyzed using two metabolomic platforms [GC-TOF MS and hydrophilic interaction chromatography (HILIC)-quad-time of flight (QTOF)/MS] for untargeted metabolomic screening, which was performed at the NIH West Coast Metabolomics Center at the University of California, Davis, as described previously (48,49). The PCA, heat maps, and pathway impact analysis of the metabolomic data were carried out using MetaboAnalyst 3.0 software (50)(51)(52). Differences in the abundance of individual metabolites were determined using a Student's t test (two-tailed, unpaired comparison).…”

Section: Methodsmentioning

confidence: 99%

Histone methyltransferase Smyd1 regulates mitochondrial energetics in the heart

Warren

Tracy

Miller

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Smyd1, a muscle-specific histone methyltransferase, has established roles in skeletal and cardiac muscle development, but its role in the adult heart remains poorly understood. Our prior work demonstrated that cardiac-specific deletion of Smyd1 in adult mice (Smyd1-KO) leads to hypertrophy and heart failure. Here we show that down-regulation of mitochondrial energetics is an early event in these Smyd1-KO mice preceding the onset of structural abnormalities. This early impairment of mitochondrial energetics in Smyd1-KO mice is associated with a significant reduction in gene and protein expression of PGC-1α, PPARα, and RXRα, the master regulators of cardiac energetics. The effect of Smyd1 on PGC-1α was recapitulated in primary cultured rat ventricular myocytes, in which acute siRNA-mediated silencing of Smyd1 resulted in a greater than twofold decrease in PGC-1α expression without affecting that of PPARα or RXRα. In addition, enrichment of histone H3 lysine 4 trimethylation (a mark of gene activation) at the PGC-1α locus was markedly reduced in Smyd1-KO mice, and Smyd1-induced transcriptional activation of PGC-1α was confirmed by luciferase reporter assays. Functional confirmation of Smyd1's involvement showed an increase in mitochondrial respiration capacity induced by overexpression of Smyd1, which was abolished by siRNA-mediated PGC-1α knockdown. Conversely, overexpression of PGC-1α rescued transcript expression and mitochondrial respiration caused by silencing Smyd1 in cardiomyocytes. These findings provide functional evidence for a role of Smyd1, or any member of the Smyd family, in regulating cardiac energetics in the adult heart, which is mediated, at least in part, via modulating PGC-1α.

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“…1B ). Likewise, a marked effect of temperature on the composition of fatty acids and water-soluble metabolites in N33 was observed in our metabolomics analysis when cells were grown under constant cold conditions at 4 °C or 10 °C [ 24 ]. We found that only a few genes were differentially up-regulated during the transient cold treatments (60 min or less; T 1 , T 2 , T 3 , and T 4 ), except for condition T 5 (4 h) which showed an intermediate response (Fig.…”

Section: Resultsmentioning

confidence: 73%

“…Cryo-protectants (metabolites and proteins) stabilize cytoplasmic macromolecules and phospholipid bilayers, prevent or reduce ice-crystal formation and freezing damage in bacterial cells at low temperatures [ 41 , 42 ]. Metabolomics analysis of Mesorhizobium strain N33 has confirmed that mannitol accumulates at constant cold growth conditions (GT 4 and GT 10 ), as do other compatible solutes (isobutyrate, sarcosine, threonine and valine) [ 24 ]. A reverse genetics experiment [ 43 ] could elucidate whether mannitol is assimilated from the culture media or synthesized de novo.…”

Section: Resultsmentioning

confidence: 99%

“…It has been shown that transcript stability (half-life) is a gene regulation strategy in response to cold shock in bacteria [ 23 ]. Our metabolomics study on Arctic Mesorhizobium strain N33 was able to identify 110 compounds involved in central carbon metabolism, essential biosynthetic pathways, secondary metabolism, and lipids under different low temperature treatments [ 24 ]. Arctic Mesorhizobium sp.…”

Section: Introductionmentioning

confidence: 99%

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Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies

et al. 2015

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BackgroundArctic Mesorhizobium strain N33 was isolated from nodules of the legume Oxytropis arctobia in Canada’s eastern Arctic. This symbiotic bacterium can grow at temperatures ranging from 0 to 30 °C, fix nitrogen at 10 °C, and is one of the best known cold-adapted rhizobia. Despite the economic potential of this bacterium for northern regions, the key molecular mechanisms of its cold adaptation remain poorly understood.ResultsUsing a microarray printed with 5760 Arctic Mesorhizobium genomic clones, we performed a partial transcriptome analysis of strain N33 grown under eight different temperature conditions, including both sustained and transient cold treatments, compared with cells grown at room temperature. Cells treated under constant (4 and 10 °C) low temperatures expressed a prominent number of induced genes distinct from cells treated to short-term cold-exposure (<60 min), but exhibited an intermediate expression profile when exposed to a prolonged cold exposure (240 min). The most prominent up-regulated genes encode proteins involved in metabolite transport, transcription regulation, protein turnover, oxidoreductase activity, cryoprotection (mannitol, polyamines), fatty acid metabolism, and membrane fluidity. The main categories of genes affected in N33 during cold treatment are sugar transport and protein translocation, lipid biosynthesis, and NADH oxidoreductase (quinone) activity. Some genes were significantly down-regulated and classified in secretion, energy production and conversion, amino acid transport, cell motility, cell envelope and outer membrane biogenesis functions. This might suggest growth cessation or reduction, which is an important strategy to adjust cellular function and save energy under cold stress conditions.ConclusionOur analysis revealed a complex series of changes associated with cold exposure adaptation and constant growth at low temperatures. Moreover, it highlighted some of the strategies and different physiological states that Mesorhizobium strain N33 has developed to adapt to the cold environment of the Canadian high Arctic and has revealed candidate genes potentially involved in cold adaptation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1611-4) contains supplementary material, which is available to authorized users.

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Metabolomic Analysis of Cold Acclimation of Arctic Mesorhizobium sp. Strain N33

Cited by 14 publications

References 55 publications

Low temperature adaptation and the effects of cryoprotectants on mesorhizobia strains

Low temperature adaptation and the effects of cryoprotectants on mesorhizobia strains

Histone methyltransferase Smyd1 regulates mitochondrial energetics in the heart

Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies

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