Metabolomic Analysis of Ethyl Acetate and Methanol Extracts of Blueberry

Abstract

Metabolite profiling of blueberry (cultivar "Spartan") was performed by extraction using different solvents, methanol and ethyl acetate, through metabolomic analysis using LC-MS/MS. Unsupervised classification method (PCA) and supervised prediction model (OPLS-DA) provided good categorization of metabolites according to the extraction solvents. Metabolites of the anthocyanin family, including delphinidin hexoside, delphinidin, 5-O-feruloylquinic acid, malvidin hexoside, malvidin-3-arabinoside, petunidin-3-arabinoside, and petunidin hexoside, were mainly detected in methanol fractions, whereas those of the flavonoid family, including chlorogenic acid, chlorogenic acid dimer, 6,8-di-C-arabinopyranosyl-luteolin, and luteolin were successfully prepared in the ethyl acetate fraction. Thus, metabolomic analysis of blueberry extracts allows for the simple profiling of whole and distinctive metabolites for future applications.

Untargeted Metabolomics of Alternaria solani-Challenged Wild Tomato Species Solanum cheesmaniae Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways

“…Leaf tissues frozen with liquid nitrogen were pulverized with an ice-cold mortar and pestle. Five grams of leaves from pathogen-challenged and unchallenged plants were extracted using ethyl acetate (100%, 20 mL) overnight to capture nonpolar to moderately polar metabolites [ 18 ]. The supernatant was separated using centrifugation (6000× g , 4 °C, 15 min) and dried on a water bath set at 55 °C to yield 1 mL of ethyl acetate extract.…”

Compositions and Contents Anthocyanins in Blueberry (Vaccinium corymbosum L.) Varieties

“…국내에서는 블루베리에 대한 과실의 수 량과 품질 등의 형태적 특성과 품종 및 가공 처리에 따라 조 추출물의 형태로 항산화 효과를 측정하는 연구가 많은 비중 을 차지하고 있다 (Jeong et al, 2012, Kim et al, 2015. 몇몇 연구에서 대사체학을 기반으로 블루베리 안토시아닌 및 페놀 화합물을 포함하는 대사체 분석 하였으나 국내산 블루베리 안토시아닌 개별 성분에 관한 연구는 미흡한 실정이다 (Jo et al, 2014 온 패턴을 나타내었으며 (Fig. 3), 동일한 단편 이온 패턴을 갖 는 성분은 타 연구 결과를 바탕으로 안토시아닌 개별 성분을 확인하였다 (Cardenosa et al, 2016;Nakajima et al, 2004).…”