Metabolomic analysis provides new insights into the heat-hardening response of Manila clam (Ruditapes philippinarum) to high temperature stress

Zhang, Yanming; Nie, Hongtao; Yan, Xiwu

doi:10.1016/j.scitotenv.2022.159430

Cited by 24 publications

(8 citation statements)

References 81 publications

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“…A reduction in amino acids in bivalve mollusks suggests physiological changes in responses to several stresses. The contents of various amino acids decreased to different degrees under environmental stressors, such as high temperatures (Zhang et al, 2023), high temperatures and hypoxia (Hu et al, 2022), and air exposure (Nguyen et al, 2020). These results were consistent with our findings, which indicated that clams had substantial variations in metabolome profiles after air exposure.…”

Section: Discussionsupporting

confidence: 90%

“…Different metabolites in response to acute hypoxia in clams have been investigated with the goal of revealing the adaptive mechanism of acute hypoxia tolerance at the metabolic level (Sun et al, 2021). Metabolic profiles revealed that high temperatures caused changes in fatty acid composition, energy metabolism, antioxidant metabolites, hydroxyl compounds, and amino acids in heat-hardened clams compared to non-hardened clams (Zhang et al, 2023). Targeted metabolomics technology revealed the adaptation mechanism to high temperature, low oxygen, and compound stress at the metabolic level in the hard clam (Mercenaria mercenaria) (Hu et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic adaptation of the clam Ruditapes philippinarum during air exposure and the positive effects of sodium nitroprusside pretreatment

Zheng,

Huo,

Huang

et al. 2023

Front. Physiol.

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The Manila clam (Ruditapes philippinarum), as one of the shellfish living in the intertidal zone, is known for its strong ability to withstand air exposure. Sodium nitroprusside (SNP), a donor of nitric oxide (NO), has been shown to be useful for antioxidant and immune regulation in aquatic animals. In this study, an untargeted metabolomics (LC–MS/MS) technique was employed for the first time in Manila clam to analyze the metabolic and histological impacts after air exposure and the positive effects of SNP pretreatment. During air exposure, a significant increase in taurine, L-glutamate, and several polyunsaturated fatty acids in clams was detected, which indicates that clams may experience inflammatory reactions, oxidative stress, and an increase in blood ammonia content. When clams were exposed to SNP for 6 h, arginine, spermine, L-glutamic acid, and glutathione content were all upregulated, indicating that the SNP exposure induced NO production and improved antioxidant capacity in clams. When the clams were exposed to air after SNP pretreatment, there were no significant differences in the levels of taurine, L-glutamate, or aliphatic acids between the experimental and control groups. Gill tissue was more severely damaged in clams directly exposed to air than in those that experienced air exposure after SNP pretreatment, especially in clams exposed to air for a long time (72 h). Both metabolomics and tissue section structure indicated that SNP pretreatment decreased the stress responses caused by air exposure in R. philippinarum. These findings provided fresh insights and a theoretical foundation for understanding the tolerance to air exposure and physiological functions of SNP (or NO) in R. philippinarum.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Metabolic adaptation of the clam Ruditapes philippinarum during air exposure and the positive effects of sodium nitroprusside pretreatment

Zheng,

Huo,

Huang

et al. 2023

Front. Physiol.

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“…Therefore, the GS and enolase were up‐regulated that indicated the southern population of R. philippinarum may be inclined to provide the energy and balance required during stress, overwintering by mobilizing pathways related to molecular stress response, and amino acid synthesis or metabolism. Both temperature acclimatization and adaptation involve adjustments through gene expression at the level of energy metabolism capacity [17, 76, 77]. In addition, lipid radical generation, as suggested by Camus et al.…”

Section: Discussionmentioning

confidence: 99%

“…Both temperature acclimatization and adaptation involve adjustments through gene expression at the level of energy metabolism capacity [17,76,77]. In addition, lipid radical generation, as suggested by TA B L E 4 List of the proteins in common between the transcriptome and proteome in response to low temperature.…”

Section: Daps Involved In Oxidative Stressmentioning

confidence: 99%

iTRAQ‐based proteomic analysis reveals proteins associated with cold stress response in two different populations of Manila clam (Ruditapes philippinarum)

Nie

Dong

et al. 2023

Proteomics

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Water temperature is one of the key environmental factors for marine ectotherms and a change in temperature beyond and organism's capacity limits can cause a series of changes to physiological state and damage to the organism. Understanding how organisms adapt to complex environments is a central goal of evolutionary biology and ecology. Ruditapes philippinarum is an ecologically and scientifically important marine bivalve species. To uncover the molecular mechanisms of acclimation of R. philippinarum to low-temperature stress, iTRAQ-based quantitative proteomics was conducted to compare the proteomes of the north and south populations of R. philippinarum under low-temperature stress. The results showed a total of 6355 and 6352 proteins were identified in two populations, respectively. Among these, 94 and 83 were differentially abundant proteins (DAPs), and most of DAPs were related to oxidationprocess, protein binding, or an integral component of membrane. According to the results of KEGG pathway enrichment analysis, most of DAPs in both populations are involved in immune-related pathways, while other population-specific significant abundance proteins of south population and north population were enriched in biosynthesis of amino acids (Enolase, Glutamine synthetase) and unsaturated fatty acids pathways (3-ketoacyl-CoA thiolase, Stearoyl-CoA desaturase), respectively, indicating that two population of clams may have different cold-stress regulation mechanisms.Our study provides new insights into different cold stress tolerance mechanisms in northern and southern populations of R. philippinarum using iTRAQ-based proteomics. This work contributes to a better understanding of molecular basis on cold stress response and adaptations, which shed lights on evolutionary biology and general ecophysiology of R. philippinarum.

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“…At the same time, HSPs can also improve the resistance to stress in the organism. Previous studies have indicated that the energy metabolism and antioxidant capacity of Ruditapes philippinarum can be improved after a brief high-temperature treatment ( Zhang et al, 2023 ). In the present study, HSP90AA1, HSPA8 [Heat shock protein family A (HSP70) member 8], DNAJC10 [DNAJ heat shock protein family (HSP40) member C10], and DNAJA1 [DNAJ heat shock protein family (HSP40) member A1] in the HSPs family had strong interactions with other proteins, and were significantly upregulated in 4 h after high-temperature stress.…”

Section: Discussionmentioning

confidence: 99%

Exploration of anti-stress mechanisms in high temperature exposed juvenile golden cuttlefish (Sepia esculenta) based on transcriptome profiling

Wang

Bao

et al. 2023

Front. Physiol.

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Sepia esculenta is a cephalopod widely distributed in the Western Pacific Ocean, and there has been growing research interest due to its high economic and nutritional value. The limited anti-stress capacity of larvae renders challenges for their adaptation to high ambient temperatures. Exposure to high temperatures produces intense stress responses, thereby affecting survival, metabolism, immunity, and other life activities. Notably, the molecular mechanisms by which larval cuttlefish cope with high temperatures are not well understood. As such, in the present study, transcriptome sequencing of S. esculenta larvae was performed and 1,927 differentially expressed genes (DEGs) were identified. DEGs were subjected to functional enrichment analyses using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The top 20 terms of biological processes in GO and 20 high-temperature stress-related pathways in KEGG functional enrichment analysis were identified. A protein-protein interaction network was constructed to investigate the interaction between temperature stress-related genes. A total of 30 key genes with a high degree of participation in KEGG signaling pathways or protein-protein interactions were identified and subsequently validated using quantitative RT-PCR. Through a comprehensive analysis of the protein-protein interaction network and KEGG signaling pathway, the functions of three hub genes (HSP90AA1, PSMD6, and PSMA5), which belong to the heat shock protein family and proteasome, were explored. The present results can facilitate further understanding of the mechanism of high temperature resistance in invertebrates and provide a reference for the S. esculenta industry in the context of global warming.

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Metabolomic analysis provides new insights into the heat-hardening response of Manila clam (Ruditapes philippinarum) to high temperature stress

Cited by 24 publications

References 81 publications

Metabolic adaptation of the clam Ruditapes philippinarum during air exposure and the positive effects of sodium nitroprusside pretreatment

Metabolic adaptation of the clam Ruditapes philippinarum during air exposure and the positive effects of sodium nitroprusside pretreatment

iTRAQ‐based proteomic analysis reveals proteins associated with cold stress response in two different populations of Manila clam (Ruditapes philippinarum)

Exploration of anti-stress mechanisms in high temperature exposed juvenile golden cuttlefish (Sepia esculenta) based on transcriptome profiling

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