Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock

Chen, Qing; Min, Ailing; Luo, Sheng; He, Jinwei; Wu, Runqin; Lin, Ximeng; Wang, Yan; He, Wen; Zhang, Yunting; Lin, Yuanxiu; Li, Mengyao; Zhang, Yong; Luo, Yunbo; Tang, Haoru; Wang, Xiaorong

doi:10.3390/ijms23169242

“…The most discerning diagnostic markers, identified via partial least squares discriminant analysis (PLSDA) and Random Forest, were found to be various flavonoids. This implicates the flavonoid pathway as the main metabolomic "barometer" of infection 11,[20][21][22]23,[24][25][26] .…”

Section: Resultsmentioning

confidence: 99%

Spatial chemistry of citrus reveals molecules bactericidal toCandidatusLiberibacter asiaticus

Aksenov,

Blacutt,

Ginnan

et al. 2024

Preprint

0

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Huanglongbing (HLB), associated with the psyllid-vectored phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), is a disease threat to all citrus production worldwide. Currently, there are no sustainable curative or prophylactic treatments available. In this study, we utilized mass spectrometry (MS)-based metabolomics in combination with 3D molecular mapping to visualize complex chemistries within plant tissues to explore how these chemistries change in vivo in HLB-impacted trees. We demonstrate how spatial information from molecular maps of branches and single leaves yields insight into the biology not accessible otherwise. In particular, we found evidence that flavonoid biosynthesis is disrupted in HLB-impacted trees, and an increase in the polyamine, feruloylputrescine, is highly correlated with an increase in disease severity. Based on mechanistic details revealed by these molecular maps, followed by metabolic modeling, we formulated and tested the hypothesis that CLas infection either directly or indirectly converts the precursor compound, ferulic acid, to feruloylputrescine to suppress the antimicrobial effects of ferulic acid and biosynthetically downstream flavonoids. Using in vitro bioassays, we demonstrated that ferulic acid and bioflavonoids are indeed highly bactericidal to CLas, with the activity on par with a reference antibiotic, oxytetracycline, recently approved for HLB management. We propose these compounds should be evaluated as therapeutics alternatives to the antibiotics for HLB treatment. Overall, the utilized 3D metabolic mapping approach provides a promising methodological framework to identify pathogen-specific inhibitory compounds in planta for potential prophylactic or therapeutic applications.

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“…The most discerning diagnostic markers, identi ed via partial least squares discriminant analysis (PLSDA) and Random Forest, were found to be various avonoids. This implicates the avonoid pathway as the main metabolomic "barometer" of infection 11,[20][21][22]23,[24][25][26] . 3D Metabolome Mapping.…”

Section: Resultsmentioning

confidence: 99%

Spatial chemistry of citrus reveals molecules bactericidal to Candidatus Liberibacter asiaticus

Aksenov,

Blacutt,

Ginnan

et al. 2024

Preprint

0

View full text Add to dashboard Cite

Huanglongbing (HLB), associated with the psyllid-vectored phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), is a disease threat to all citrus production worldwide. Currently, there are no sustainable curative or prophylactic treatments available. In this study, we utilized mass spectrometry (MS)-based metabolomics in combination with 3D molecular mapping to visualize complex chemistries within plant tissues to explore how these chemistries change in vivo in HLB-impacted trees. We demonstrate how spatial information from molecular maps of branches and single leaves yields insight into the biology not accessible otherwise. In particular, we found evidence that flavonoid biosynthesis is disrupted in HLB-impacted trees, and an increase in the polyamine, feruloylputrescine, is highly correlated with an increase in disease severity. Based on mechanistic details revealed by these molecular maps, followed by metabolic modeling, we formulated and tested the hypothesis that CLas infection either directly or indirectly converts the precursor compound, ferulic acid, to feruloylputrescine to suppress the antimicrobial effects of ferulic acid and biosynthetically downstream flavonoids. Using in vitro bioassays, we demonstrated that ferulic acid and bioflavonoids are indeed highly bactericidal to CLas, with the activity on par with a reference antibiotic, oxytetracycline, recently approved for HLB management. We propose these compounds should be evaluated as therapeutics alternatives to the antibiotics for HLB treatment. Overall, the utilized 3D metabolic mapping approach provides a promising methodological framework to identify pathogen-specific inhibitory compounds in planta for potential prophylactic or therapeutic applications.

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“…Shuzhen No. 1 (CjSz), a new citrus rootstock, has proven reliable against various abiotic and biotic stresses [ 26 , 27 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

The Effects of a New Citrus Rootstock Citrus junos cv. Shuzhen No. 1 on Performances of Ten Hybrid Citrus Cultivars

He,

Chai,

Xie

et al. 2024

Plants

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The importance of rootstock in citrus production lies in its crucial role in determining tree growth, environmental stress tolerance, and fruit quality. Citrus junos Siebold ex Tanaka cv. Shuzhen No. 1, a recently developed rootstock, demonstrates excellent graft compatibility and abiotic stress tolerance. The objective of this study was to assess ten hybrid citrus cultivars grafted onto two C. junos rootstock selections, with the aim of determining the potential for industrial utilization of the new citrus rootstock. All graft junctions are mature and well established. Vigorous growth characterized all ten citrus cultivars on Shuzhen No. 1, with the largest tree’s height reaching 280.33 cm (Wogan scion) and the widest scion’s diameter being 67.52 cm (Chunjian scion). However, the scion-to-rootstock diameter ratio was the lowest at 0.62 (Chunxiang scion). C. junos rootstock selections significantly affected fruit weight (five of ten scions) and fruit color (seven of ten scions) but had negligible impact on peel thickness (nine of ten scions). Furthermore, rootstock type had a significant influence on fruit quality. In conclusion, our findings indicate strong graft compatibility between all scions and C. junos rootstocks, which can impact overall size and fruit quality. Based on these results, Shuzhen No. 1 is recommended as a valuable citrus rootstock.

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Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock

Cited by 10 publications

References 70 publications

Spatial chemistry of citrus reveals molecules bactericidal toCandidatusLiberibacter asiaticus

Spatial chemistry of citrus reveals molecules bactericidal toCandidatusLiberibacter asiaticus

Spatial chemistry of citrus reveals molecules bactericidal to Candidatus Liberibacter asiaticus

The Effects of a New Citrus Rootstock Citrus junos cv. Shuzhen No. 1 on Performances of Ten Hybrid Citrus Cultivars

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