Metabolomic and physico-chemical approach unravel dynamic regulation of calcium in sweet cherry fruit physiology

Michailidis, Michail; Karagiannis, Evangelos; Tanou, Georgia; Karamanoli, Katerina; Lazaridou, Athina; Matsi, Theodora; Molassiotis, Athanassios

doi:10.1016/j.plaphy.2017.05.005

Cited by 83 publications

(51 citation statements)

References 38 publications

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“…6b). A clear connection among xylose, arabinose and cracking expression patterns in sweet cherry has been recently established [13], further supporting the role of these metabolites in fruit cracking. Our data also demonstrated that several soluble alcohols such as glycerol, mannitol and inositol, were negatively correlated, independently of the applied method, with cracking rate (Fig.…”

Section: Physiological Traits and Skin Metabolites In Relations To Crmentioning

confidence: 73%

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Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Michailidis

Karagiannis

Tanou³

et al. 2019

Preprint

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Background: Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivo cracking assay, the ‘Christensen method’ (cracking evaluation following fruit immersion in water) is commonly used; however, this test has been questioned. Herein, we have designed and evaluated a cracking protocol, named ‘Waterfall method’, in which fruits are continuously wetted under controlled conditions. Results: The application of this method alone, or in combination with ‘Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC-MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index–metabolite correlations were identified. Conclusions: We have established an efficient cracking protocolwhich may facilitate breeding for new sweet cherry cultivars with high resistance tocracking.

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Section: Physiological Traits and Skin Metabolites In Relations To Crmentioning

confidence: 73%

Section: Textural Properties Of Skin Tissuementioning

confidence: 99%

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Michailidis

Karagiannis

Tanou³

et al. 2019

Preprint

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“…It is widely accepted that foliar sprays of calcium solutions have a positive effect in preventing fruit cracking in sweet cherry (Erogul, 2014;Kafle et al, 2016;Michailidis et al, 2017), pomegranate (Bakeer, 2016;Sheikh & Manjula, 2012) and navel orange (Chen et al, 2014;Wen & Shi, 2012). The present study showed that dipping grape berries in calcium solution also significantly reduced fruit cracking and the effect was superior to that of foliar sprays (Table S1).…”

Section: Dipping Fruit With 5 G L −1 Cacl 2 Is Effective To Reduce Frmentioning

confidence: 99%

“…Therefore, to prevent localized calcium deficiency and promote fruit quality, it is necessary to supply calcium during the ripening stage. Preharvest applications of calcium-containing solutions decrease the incidence of fruit cracking in sweet cherry (Erogul, 2014;Michailidis et al, 2017), pomegranate (Davarpanah et al, 2018, and navel orange (Chen et al, 2014;Wen & Shi, 2012). However, the effect of calcium on grape berry cracking and its physiological and molecular mechanisms remain largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

Effect of calcium on relieving berry cracking in grape (Vitis viniferaL.) ‘Xiangfei’

Zhu

Miao

et al. 2020

PeerJ

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Fruit cracking is a physiological disorder in many plant species that leads to severe economic losses. The aim of this study was to investigate the effect of calcium on fruit cracking and explore the underlying mechanisms. We studied the effect of exogenous calcium on grape berry cracking, calcium absorbance and distribution, and cell wall metabolism in the cracking-susceptible cultivar ‘Xiangfei’. Calcium significantly reduced the frequency of fruit cracking, increased the break force of the berry skin, and stimulated storage of calcium. In addition, calcium increased the content of protopectin and inhibited the increase in content of water-soluble pectin, by regulating the transcription and activities of enzymes associated with cell wall metabolism. Taken together, the results indicated that dipping grape berries in calcium solution is effective in preventing fruit cracking by stimulating calcium uptake, inhibiting cell wall disassembly, and promoting cell wall strengthening.

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“…Texture Analyzer (Stable Microsystems, Godalming, Surrey, UK) as described in detail [13] following a four sides skin penetration protocol (upper side, suture, apical and near stem) (Additional file 2: Supplementary Table 2). Total skin penetration was calculated as the average of four sides measurements (Additional file 2: Supplementary Table 2).…”

Section: Textural Properties Of Skin Tissuementioning

confidence: 99%

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Michailidis

Karagiannis

Tanou³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivocracking assay, the 'Christensen method' (cracking evaluation following fruit immersion in water) is commonly used; however, this test does not adequately simulate environmental conditions.Herein, we have designed and evaluated a cracking protocol, named 'Waterfall method', in which fruits are continuously wetted under controlled conditions. Results: The application of this method alone, or in combination with 'Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC-MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index-metabolite correlations were identified. Conclusions:We have established an alternative/complementary method of cherry cracking characterization alongside to Christiansen assay.Additional file 1: Supplementary Table S1. Physiological traits of seventeen cherry cultivars at harvest and MANOVA output. Supplementary Table S2. Texture properties, main cracking of cultivars at harvest and MANOVA output. Additional file 2:Additional file 3: Supplementary Table S3. Quantitative results of skin primary metabolite analysis and MANOVA output. Supplementary Table S4. Quantitative results of skin secondary metabolite analysis and MANOVA output. Additional file 4:

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Metabolomic and physico-chemical approach unravel dynamic regulation of calcium in sweet cherry fruit physiology

Cited by 83 publications

References 38 publications

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Effect of calcium on relieving berry cracking in grape (Vitis viniferaL.) ‘Xiangfei’

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

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