Metabolomic approaches for the identification of flavonoids associated with weed suppression in selected Hardseeded annual pasture legumes

Latif, Sajid; Gurusinghe, Saliya; Weston, Paul A.; Quinn, Jane C.; Piltz, John; Weston, Leslie A.

doi:10.1007/s11104-019-04225-4

Cited by 19 publications

(11 citation statements)

References 62 publications

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“…Monocultures of selected pasture legumes listed in Table 4 were established in 2016 and 2017 (Experiment 1) at the Charles Sturt University research farm in Wagga Wagga, NSW, Australia (35.04° S, 147.36° E) on a red sodosol soil [ 75 ]. Each planting was arranged as a randomized complete block design with five replications.…”

Section: Methodsmentioning

confidence: 99%

“…Each planting was arranged as a randomized complete block design with five replications. Individual pasture legumes were established in plots by direct-seeding with a drill adapted for small-seeded legumes in late May to early June of each year, with plots measuring approximately 4 × 20 m. Following on from experimentation to assess legume establishment and performance [ 75 ], above-ground plant tissues (leaf, stem, and flower) were collected from established plots in the third week of October in 2016 and 2017 after >50% flowering was achieved in each species. This corresponded to the growth stage in which peak concentrations of phytoestrogen have been reported in vegetative tissue [ 76 ].…”

Section: Methodsmentioning

confidence: 99%

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Metabolic Profiling Provides Unique Insights to Accumulation and Biosynthesis of Key Secondary Metabolites in Annual Pasture Legumes of Mediterranean Origin

et al. 2020

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Annual legumes from the Mediterranean region are receiving attention in Australia as alternatives to traditional pasture species. The current study employed novel metabolic profiling approaches to quantify key secondary metabolites including phytoestrogens to better understand their biosynthetic regulation in a range of field-grown annual pasture legumes. In addition, total polyphenol and proanthocyanidins were quantified using Folin–Ciocalteu and vanillin assays, respectively. Metabolic profiling coupled with biochemical assay results demonstrated marked differences in the abundance of coumestans, flavonoids, polyphenols, and proanthocyanidins in annual pasture legume species. Genetically related pasture legumes segregated similarly from a chemotaxonomic perspective. A strong and positive association was observed between the concentration of phytoestrogens and upregulation of the flavonoid biosynthetic pathway in annual pasture legumes. Our findings suggest that evolutionary differences in metabolic dynamics and biosynthetic regulation of secondary metabolites have logically occurred over time in various species of annual pasture legumes resulting in enhanced plant defense.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Metabolic Profiling Provides Unique Insights to Accumulation and Biosynthesis of Key Secondary Metabolites in Annual Pasture Legumes of Mediterranean Origin

et al. 2020

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“…The seedlings with a radicle length of >1 mm were considered as having germinated. The percentage inhibition of germination and the length of radicles and hypocotyls relative to the control was calculated [ 52 ]. The pure parthenin compound (University of Hohenheim, Germany) was also evaluated against the garden cress at a range of concentrations (200 to 1000 μM).…”

Section: Methodsmentioning

confidence: 99%

Toxic Potential and Metabolic Profiling of Two Australian Biotypes of the Invasive Plant Parthenium Weed (Parthenium hysterophorus L.)

Bajwa

Weston

Gurusinghe

et al. 2020

Toxins

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Parthenium weed (Parthenium hysterophorus L.) is an invasive plant species in around 50 countries and a ‘Weed of National Significance’ in Australia. This study investigated the relative toxicity of the leaf, shoot and root extracts of two geographically separate and morphologically distinct biotypes of parthenium weed in Queensland, Australia. Parthenium weed exhibited higher phytotoxic, cytotoxic and photocytotoxic activity in leaf tissue extracts in contrast to shoot and root. The germination and seedling growth of a dicot species (garden cress) were inhibited more than those of a monocot species (annual ryegrass) using a phytotoxicity bioassay. The cytotoxicity of leaf extracts was assessed in a mouse fibroblast cell suspension assay and increased under high ultraviolet A(UV-A) radiation. A major secondary metabolite, parthenin, was found in abundance in leaf extracts and was positively correlated with cytotoxicity but not with photocytotoxicity or phytotoxicity. Ambrosin and chlorogenic acid were also detected and were positively correlated with germination inhibition and the inhibition of radicle elongation, respectively. In addition, other currently unidentified compounds in the leaf extracts were positively correlated with phytotoxicity, cytotoxicity and photocytotoxicity with two to three molecules strongly correlated in each case. Both parthenium weed biotypes investigated did not differ with respect to their relative toxicity, despite their reported differences in invasive potential in the field. This suggests that secondary chemistry plays a limited role in their invasion success.

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“…Similarly, metabolic profiling was carried out to decipher the weed suppressive metabolites in different annual legumes. The UHPLC QTOF-MS-based technique was executed and found an abundance of flavonoids that can be used to suppress different weeds [192].…”

Section: Elucidation Of Biotic Stress Resistance In Plantsmentioning

confidence: 99%

Metabolomics: A Way Forward for Crop Improvement

et al. 2019

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Metabolomics is an emerging branch of “omics” and it involves identification and quantification of metabolites and chemical footprints of cellular regulatory processes in different biological species. The metabolome is the total metabolite pool in an organism, which can be measured to characterize genetic or environmental variations. Metabolomics plays a significant role in exploring environment–gene interactions, mutant characterization, phenotyping, identification of biomarkers, and drug discovery. Metabolomics is a promising approach to decipher various metabolic networks that are linked with biotic and abiotic stress tolerance in plants. In this context, metabolomics-assisted breeding enables efficient screening for yield and stress tolerance of crops at the metabolic level. Advanced metabolomics analytical tools, like non-destructive nuclear magnetic resonance spectroscopy (NMR), liquid chromatography mass-spectroscopy (LC-MS), gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), and direct flow injection (DFI) mass spectrometry, have sped up metabolic profiling. Presently, integrating metabolomics with post-genomics tools has enabled efficient dissection of genetic and phenotypic association in crop plants. This review provides insight into the state-of-the-art plant metabolomics tools for crop improvement. Here, we describe the workflow of plant metabolomics research focusing on the elucidation of biotic and abiotic stress tolerance mechanisms in plants. Furthermore, the potential of metabolomics-assisted breeding for crop improvement and its future applications in speed breeding are also discussed. Mention has also been made of possible bottlenecks and future prospects of plant metabolomics.

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Metabolomic approaches for the identification of flavonoids associated with weed suppression in selected Hardseeded annual pasture legumes

Cited by 19 publications

References 62 publications

Metabolic Profiling Provides Unique Insights to Accumulation and Biosynthesis of Key Secondary Metabolites in Annual Pasture Legumes of Mediterranean Origin

Metabolic Profiling Provides Unique Insights to Accumulation and Biosynthesis of Key Secondary Metabolites in Annual Pasture Legumes of Mediterranean Origin

Toxic Potential and Metabolic Profiling of Two Australian Biotypes of the Invasive Plant Parthenium Weed (Parthenium hysterophorus L.)

Metabolomics: A Way Forward for Crop Improvement

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