Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Abstract

Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

High Intensity Pulsed Electric Field Procesiing of Foods - A Review

“…Moderate-intensity pulsed electric fields (MIPEF) permeabilise tissue structures, thus improving intracellular metabolite extraction (SolivaFortuny et al, 2009) and enhancing drying efficiency (De Vito et al, 2008). Metabolic responses of plant cells (Cai et al, 2011) and tissues (Galindo et al, 2009) upon the application of MIPEF have been studied. The analysis of potato demonstrated that, 24 h after the application of MIPEF, tissue metabolism showed MIPEF-specific stress responses, characterised by changes in the hexose pool that may involve starch and ascorbic acid degradation (Galindo et al, 2009).…”

“…Metabolic responses of plant cells (Cai et al, 2011) and tissues (Galindo et al, 2009) upon the application of MIPEF have been studied. The analysis of potato demonstrated that, 24 h after the application of MIPEF, tissue metabolism showed MIPEF-specific stress responses, characterised by changes in the hexose pool that may involve starch and ascorbic acid degradation (Galindo et al, 2009). MIPEF-induced stresses could include a burst of ROS, which are endogenous signal components required for synthesis of secondary metabolites, such as polyphenols or carotenoids, which are known to be part of the defence response of plants to stress (Shohael et al, 2006).…”

“…Moderate-intensity pulsed electric fields (MIPEF) have been studied as possible treatments to enhance the generation of secondary plant metabolites by inducing stress reactions. It has been described that MIPEF-induced stress affects metabolism with the consequent generation of reactive oxygen species (ROS) (Galindo et al, 2009 MIPEF PROCESSING OF TOMATOES MIPEF treatments were conducted in batch mode using anequipment manufactured by Physics International (San Leandro, CA, USA), which can deliver pulses from a capacitor of 0.1 lF with an exponential decaying waveform. A stainless steel parallel plate treatment chamber was used.…”

Research and Therapeutic Innovation: Tissue Resonance InterferoMeter Probe in Early Detection-Screening for Rectal Cancer

“…It is a research tool that can detect and monitor unidentified compounds as well as identified metabolites that play important roles in metabolism and physiology (Kaplan et al, 2004). For example metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress (Galindo et al, 2009). …”

Metabolomics - Useful Tool for Study of Plant Responses to Abiotic Stresses

“…Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nuclei. Metabolic profiling of data obtained through GC-(TOF)-MS and UPLC-(TOF)-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of pulsed electric fields potato (PEF)-specific responses in the potato metabolism were characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation [89].…”