Metabolomic profiling and antioxidant potential of three fungal endophytes derived from <i>Artemisia annua</i> and <i>Medicago sativa</i>

Sayed, Ahmed M.; Sherif, Noheir H; El-Gendy, Ahmed O.; Shamikh, Yara I.; Ali, Asmaa T.; Attia, Eman Zekry; El-Katatny, Mo'men H.; Khalifa, Basma Ali; Hassan, Hossam M.; Abdelmohsen, Usama Ramadan

doi:10.1080/14786419.2020.1831495

Cited by 14 publications

(6 citation statements)

References 8 publications

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“…da Silva et al [ 219 ] reported that, the extract of endophytic A. nidulans isolated from Passiflora Incarnate has potential antioxidant activity. Moreover, A. oryzae and A. terreus have antioxidant activity [ 221 ]. Likewise, antioxidant activity of endophytic penicillium spp.…”

Section: Biomedical Applications Of Fungal Endophytesmentioning

confidence: 99%

Bioactive compounds and biomedical applications of endophytic fungi: a recent review

Hashem,

Attia,

Kandil

et al. 2023

Microb Cell Fact

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Human life has been significantly impacted by the creation and spread of novel species of antibiotic-resistant bacteria and virus strains that are difficult to manage. Scientists and researchers have recently been motivated to seek out alternatives and other sources of safe and ecologically friendly active chemicals that have a powerful and effective effect against a wide variety of pathogenic bacteria as a result of all these hazards and problems. In this review, endophytic fungi and their bioactive compounds and biomedical applications were discussed. Endophytes, a new category of microbial source that can produce a variety of biological components, have major values for study and broad prospects for development. Recently, endophytic fungi have received much attention as a source for new bioactive compounds. In addition, the variety of natural active compounds generated by endophytes is due to the close biological relationship between endophytes and their host plants. The bioactive compounds separated from endophytes are usually classified as steroids, xanthones, terpenoids, isocoumarins, phenols, tetralones, benzopyranones and enniatines. Moreover, this review discusses enhancement methods of secondary metabolites production by fungal endophytes which include optimization methods, co-culture method, chemical epigenetic modification and molecular-based approaches. Furthermore, this review deals with different medical applications of bioactive compounds such as antimicrobial, antiviral, antioxidant and anticancer activities in the last 3 years.

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Section: Biomedical Applications Of Fungal Endophytesmentioning

confidence: 99%

Bioactive compounds and biomedical applications of endophytic fungi: a recent review

Hashem,

Attia,

Kandil

et al. 2023

Microb Cell Fact

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“…Molecular identification of the most active fungal isolated strains recovered from Nigella sativa seeds was achieved by sequence analysis of the fungal internal transcribed spacer (ITS) region including ITS1, the 5.8 S rRNA gene, and ITS2 sequences according to [21]. In brief, DNA was extracted from fungal biomass using the MasterPure Yeast DNA extraction kit (epientre, Madison, Wisconsin).…”

Section: Molecular Identification and Phylogenetic Analysismentioning

confidence: 99%

Phytochemical analysis and anti-infective potential of fungal endophytes isolated from Nigella sativa seeds

Shady,

Sobhy,

Mostafa

et al. 2023

BMC Microbiol

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Endophytic fungi, particularly from higher plants have proven to be a rich source of antimicrobial secondary metabolites. The purpose of this study is to examine the antimicrobial potential of three endophytic fungi Aspergillus sp. SA1, Aspergillus sp. SA2, and Aspergillus sp. SA3, cultivated from Nigella sativa seeds against Staphylococcus aureus (ATCC 9144), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 13883), MRSA (ATCC 33591), and human pathogen Candida albicans (ATCC 10231). Furthermore, the most active cultivated endophytic fungi were molecularly identified via internal transcribed spacer (ITS) sequencing. HR-ESIMS guided approach has been used successfully in chemical profiling of 26 known bioactive secondary metabolites (1–26), which belongs to different classes of natural compounds such as polyketides, benzenoids, quinones, alcohols, phenols or alkaloids. Finally, in-silico interactions within active site of fungal Cyp51 and bacterial DNA gyrase revealed possibility of being a hit-target for such metabolites as antimicrobials.

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“…Statistical analyses were carried out by using MetaboAnalyst software on the different variables (Sayed et al, 2020). Principal Component Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA) were performed to reveal the differences in the metabolic composition of the examined samples.…”

Section: Statistical and Multivariate Data Analysesmentioning

confidence: 99%

Cytotoxic potential ofNephtheasp.-derived actinomycetes supported by metabolomics analysis

Abdelhafez

Fahim

Rateb

et al. 2022

Natural Product Research

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Soft corals and associated microorganisms are known to produce leads for anticancer drugs. Keeping this in mind, Nephthea sp.; a Red Sea soft coral was investigated for the first time using the OSMAC approach. Two isolates, Streptomyces sp. UR63 and Micrococcus sp. UR67 were identified. Their extracts revealed the presence of alkaloids, macrolides, quinones, fatty acids, and terpenoids. Further comparison through a set of multivariate data analyses revealed their unique chemical profiles. The extracts displayed inhibitory potencies against HepG-2, Caco-2, and MCF-7 tumor cell lines with IC50 values ranging from 11.4 to 38.7 μg/mL when compared with the positive control, doxorubicin.The study not only highlights the cytotoxic potential of soft coral-associated actinomycetes but also shows the advantage of using the OSMAC approach in this regard.

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Metabolomic profiling and antioxidant potential of three fungal endophytes derived from Artemisia annua and Medicago sativa

Cited by 14 publications

References 8 publications

Bioactive compounds and biomedical applications of endophytic fungi: a recent review

Bioactive compounds and biomedical applications of endophytic fungi: a recent review

Phytochemical analysis and anti-infective potential of fungal endophytes isolated from Nigella sativa seeds

Cytotoxic potential ofNephtheasp.-derived actinomycetes supported by metabolomics analysis

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