Metabolomic Profiling of Human Spermatozoa in Idiopathic Asthenozoospermia Patients Using Gas Chromatography-Mass Spectrometry

Zhao, Kai; Zhang, Jianzhong; Xu, Zhen; Xu, Yue; Xu, Aiming; Chen, Wei; Miao, Chenkui; Liu, Shouyong; Wang, Zengjun; Jia, Ru

doi:10.1155/2018/8327506

Cited by 35 publications

(39 citation statements)

References 21 publications

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“…To the best of our knowledge, only a few studies have been performed with the metabolomics approach [30] on a cohort of patients affected by oligozoospermia and, for this reason, it is not simple to explain the biological meaning of the metabolites by comparing our study with others. Zhao et al applied an untargeted GC-MS analysis on patients affected by idiopathic asthenozoospermia, and they found a different panel of altered metabolites compared with our study based on the different analytical technique used [31]. Moreover, Jayaraman et al performed an NMR analysis of seminal fluids of patients with different forms of male infertility, highlighting the presence of a difference in biochemical profiles, with a specific emphasis on idiopathic infertility where the level of lysine could be of diagnostic use [17].…”

Section: Discussionmentioning

confidence: 49%

Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans

et al. 2020

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Infertility affects 12–15% of couples worldwide, and male factors are the cause of nearly half of all cases. Studying seminal fluid composition could lead to additional diagnostic accuracy and a better understanding of the pathophysiology of male factor infertility. Metabolomics offers a new opportunity to evaluate biomarkers and better understand pathological mechanisms. The aim of the study was to identify new markers or therapeutic targets to improve outcomes in male factor or idiopathic infertility patients. Semen samples were obtained from 29 men with a normal spermogram test, and from 18 oligozoospermic men. Samples were processed and analyzed by Nuclear Magnetic Resonance spectroscopy and, subsequently, multivariate and univariate statistical analyses. Receiving Operator Curves (ROC) and Spearman correlations were also performed. An Orthogonal Partial Least Square Discriminant Analysis supervised multivariate model was devised to compare the groups. The levels of fructose, myo-inositol, aspartate and choline were altered. Moreover, Spearman Correlation associated fructose, aspartate and myo-inositol with the total amount of spermatozoa, total motile spermatozoa, % of immotility and % of “in situ” spermatozoic motility respectively. NMR-based metabolomics allowed the identification of a specific metabolic fingerprint of the seminal fluids of patients affected by oligozoospermia.

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Section: Discussionmentioning

confidence: 49%

Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans

et al. 2020

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“…Amino acids like lysine and valine were also decreased with patients with OAT compared to men with normozoospermia. In agreement with this study, previous studies also have reported decreased valine levels in patients with idiopathic asthenozoospermia (Qiao et al ., ; Zhao et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics analysis of seminal plasma in patients with idiopathic Oligoasthenoteratozoospermia using high‐resolution NMR spectroscopy

et al. 2019

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Background Male infertility is a global health issue caused by a combination of different factors. Specialists generally rely on semen analysis to diagnose male infertility. However, it is known that diagnostic semen analysis fails to identify about 50% of male infertility disorders. Recently, metabolomics has been proven to be a powerful technique for the diagnosis of different diseases. Objective To determine whether metabolites could be used as potential biomarkers for the diagnosis of male factor infertility through comparing seminal plasma samples from infertile men with oligoasthenoteratozospermia (OAT) and samples from normozoospermic controls. Materials and methods This study utilized high‐resolution 1H NMR spectroscopy to reveal whether the metabolomic changes of seminal plasma obtained from 31 patients with oligoasthenoteratozospermia (OAT) are different from the ones obtained from 28 normozoospermic controls. Results Multivariate statistical analysis of NMR data concluded that the metabolomic profile of samples from patients with OAT exhibits statistically significant differences when compared to the controls. The differences were based on the metabolites lactate, citrate, lysine, arginine, valine, glutamine, creatinine, α‐ketoglutaric acid, spermine, putrescine, and tyrosine. Except the tyrosine, levels of the above metabolites were significantly decreased in patients with OAT compared to the controls. The levels of citrate, choline, spermine, putrescine, α‐ketoglutaric acid, valine, and tyrosine were significantly different (p < 5 × 10−4) between two groups. On the other hand, levels of lactate, creatinine, lysine, arginine, and glutamine were also statistically significant (0.001 < p < 0.05). However, considering the p‐values, the physiological relevance of these metabolites may be lower when compared to the others. A PLS‐DA model built on the NMR data achieved 89.29% sensitivity and 93.55% specificity results in a leave‐one‐out cross‐validation process. Discussion and conclusion 1H NMR spectroscopy‐based metabolomic analysis could be used as a diagnostic tool for the diagnosis of oligoasthenoteratozospermia.

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“…Sperm utilizes two metabolic pathways for producing energy, namely glycolysis and oxidative phosphorylation [13]. The sperm's preference for energy metabolic pathways is believed to be species-specific.…”

Section: Introductionmentioning

confidence: 99%

miR-26a is Involved in Glycometabolism and Affects Boar Sperm Viability by Targeting PDHX

Wang

Liang

Chang

et al. 2020

Cells

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miR-26a is associated with sperm metabolism and can affect sperm motility and apoptosis. However, how miR-26a affects sperm motility remains largely unknown. Our previous study indicated that the PDHX gene is predicted to be a potential target of miR-26a, which is responsible for pyruvate oxidative decarboxylation which is considered as a key step for connecting glycolysis with oxidative phosphorylation. In this study, we first reported a potential relationship between miR-26a and PDHX and their expressions in fresh, frozen-thawed, and epididymal boar sperm. Then, sperm viability and survival were determined after transfection of miR-26a. mRNA and protein expression level of PDHX in the liquid-preserved boar sperm after transfection were also determined by RT-qPCR and Western Blot (WB). Our results showed that expression level of PDHX was significantly increased during sperm transit from epididymal caput to corpus and cauda. Similarly, expression of PDHX was significantly higher (P < 0.05) in fresh sperm as compared to epididymal cauda and frozen-thawed sperm. However, the expression of miR-26a in epididymal corpus sperm was significantly higher (P < 0.05) than that of caput and cauda sperm. Furthermore, after transfection of boar sperm with miR-26a mimic and inhibitor under liquid storage, the lowest and highest sperm viability was observed in miR-26a mimic and inhibitor treatment (P < 0.05), respectively. The protein levels of PDHX, after 24 and 48 h of transfection of miR-26a mimics and inhibitor, were notably decreased and increased (P < 0.05), respectively, as compared to negative control (NC) group. In conclusion, the novel and enticing findings of our study provide a reasonable evidence that miR-26a via PDHX, a link between glycolysis and oxidative phosphorylation, could regulate the glycometabolic pathway which eventually affect boar sperm viability and survival.

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Metabolomic Profiling of Human Spermatozoa in Idiopathic Asthenozoospermia Patients Using Gas Chromatography-Mass Spectrometry

Cited by 35 publications

References 21 publications

Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans

Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans

Metabolomics analysis of seminal plasma in patients with idiopathic Oligoasthenoteratozoospermia using high‐resolution NMR spectroscopy

miR-26a is Involved in Glycometabolism and Affects Boar Sperm Viability by Targeting PDHX

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