Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models

Abstract

Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p < 0.001) intestinal segments of rat pre-contracted with acetylcholine (ACh) (10−5 M). Pre-incubation of intestinal segments with 100 µg/mL MIE significantly reduced (p < 0.001) the contraction to H2O2. Similar effects were observed with mangiferin and quercetin (10−5 M; p < 0.05) but not for gallic acid. Chronic treatment of rats with MIE (50 mg/kg) for 28 days significantly reduced (p < 0.001) the H2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.

Column adsorption of biological oxygen demand, chemical oxygen demand and total organic carbon from wastewater by magnetite nanoparticles-zeolite A composite

“…To confirm and authenticate the fabrication and stability of NPs, UV–Vis absorption spectroscopy is predominantly utilized due to the sensitivity of SPR absorbance wavelength and shape towards the particle size, morphology, agglomeration, metallic dielectric functions and the environment [ 66 ]. The MIL extract shows a specific peak at 280 nm which relates to the presence of polyphenolic compounds [ 68 ]. After the synthesis period, the maximum absorption peak monitored by UV–Vis spectroscopy was reduced to 259 nm as shown in Fig.…”

Metabolic Profiling of Vasorelaxant Extract from Malvaviscus arboreus by LC/QTOF‐MS

“…Commonly, bioactive compounds isolated from medicinal plants are identified, elucidated, and standardized by spectroscopic and spectrometric techniques. Other organic extracts with phenolic compounds and pure phenolic compounds have been reported with interesting vasorelaxant bioactivity in rat aorta preparations [12,13] . In this sense, pentacyclic triterpenes, such as betulinic acid, are considered interesting compounds due to their vasorelaxant activity [14] .…”

Antispasmodic Effect of Valeriana pilosa Root Essential Oil and Potential Mechanisms of Action: Ex Vivo and In Silico Studies

“…Animals were sacrificed by cervical dislocation. A portion of the ileum (2.5 cm), without considering the 10 cm nearest to the ileocecal valve, was removed and placed into a petri dish containing Tyrode’s solution (concentrations in mM): NaCl 136.9; KCl 2.68; CaCl 2 1.8; MgCl 2 1.05; NaHCO 3 11.9; NaH 2 PO 4 0.42; and D-glucose 5.55 [ 22 ]. Ileum fragments were placed into an isolated organ chamber filled with 25 mL of Tyrode´s solution.…”

“…The contractility of the rat ileum in response to VPEO was assessed through cumulative dose–response experiments. A series of seven distinct concentrations of VPEO (1, 10, 100, 250, 500, 750, and 1000 μg/mL) were administered in 5 min intervals [ 22 ].…”